Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,286,859	T→C	intergenic (+333/+207)	narI → / ← rttR	nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,286,859	0	T	C	100.0%	70.8 / NA	25	intergenic (+333/+207)	narI/rttR	nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/13);  total (12/13)

AAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGA  >  NC_000913/1286810‑1286988                                                  |                                                                                                                                  aaGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCgg                                          <  1:1337251/139‑1 (MQ=17)   gCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGa                                                                                                       <  2:636131/76‑1 (MQ=11)   gCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGa                                                                                                       >  1:636131/1‑76 (MQ=11)    cTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCGTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCaa                                       <  1:871503/139‑1 (MQ=12)               tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCtt                                                           <  1:1448842/108‑1 (MQ=255)               tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCtt                                                           >  2:1448842/1‑108 (MQ=9)                      atGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGaaa                                                                                                           >  2:7478/2‑53 (MQ=14)                      atGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGaaa                                                                                                           <  1:7478/52‑1 (MQ=14)                         ggCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                                           >  2:723070/1‑66 (MQ=255)                         ggCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                                           <  1:723070/66‑1 (MQ=255)                          gCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtgg                                                                                                 <  2:92236/59‑1 (MQ=255)                          gCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtgg                                                                                                 >  1:92236/1‑59 (MQ=255)                               atatTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGTCGaa            >  1:760428/1‑139 (MQ=16)                                   ttGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTg                                                                                                        <  2:402171/43‑1 (MQ=255)                                   ttGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTg                                                                                                        >  1:402171/1‑43 (MQ=255)                                   ttGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtgg                                                                                                 >  2:542086/1‑50 (MQ=255)                                   ttGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtgg                                                                                                 <  1:542086/50‑1 (MQ=255)                                   ttGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg                                                                >  2:1227181/1‑83 (MQ=255)                                   ttGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg                                                                <  1:1227181/83‑1 (MQ=255)                                   ttGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCtt        >  2:1486158/1‑139 (MQ=255)                                         gCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGa  <  2:1348522/139‑1 (MQ=255)                                          cGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                                           <  1:771239/49‑1 (MQ=255)                                          cGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                                           >  2:771239/1‑49 (MQ=255)                                          cGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCtt                                                           <  2:1502975/81‑1 (MQ=255)                                          cGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCtt                                                           >  1:1502975/1‑81 (MQ=255)                                                  |                                                                                                                                  AAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGA  >  NC_000913/1286810‑1286988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑