Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,458,617 A→G A118A (GCA→GCG)  paaG → 1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,458,6170AG100.0% 200.6 / NA 56A118A (GCA→GCG) paaG1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/28);  total (28/28)

AGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCACTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTG  >  NC_000913/1458485‑1458752
                                                                                                                                    |                                                                                                                                       
aGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTgggg                                                                                                                                   >  2:895328/1‑139 (MQ=255)
 ggCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTggggg                                                                                                                                  >  2:409827/1‑139 (MQ=255)
   ccccGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGc                                                                                                                                >  1:555058/1‑139 (MQ=255)
      cGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGAc                                                                                                                             >  2:1578200/1‑139 (MQ=255)
      cGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGAc                                                                                                                             >  2:1189978/1‑139 (MQ=255)
        cACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACAt                                                                                                                           >  1:1004754/1‑139 (MQ=255)
        cACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACAt                                                                                                                           >  2:791488/1‑139 (MQ=255)
        cACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACAt                                                                                                                           >  1:1312632/1‑139 (MQ=255)
                       tGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTggggg                                                                                                                                  <  2:240181/117‑1 (MQ=255)
                       tGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTggggg                                                                                                                                  >  1:240181/1‑117 (MQ=255)
                       tGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCg                                                                                                            <  1:1578200/139‑1 (MQ=255)
                        gTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGt                                                                                                           <  2:411658/139‑1 (MQ=255)
                        gTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGt                                                                                                           >  1:917306/1‑139 (MQ=255)
                        gTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGt                                                                                                           >  1:185491/1‑139 (MQ=255)
                            gTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAg                                                                                                       <  2:185491/139‑1 (MQ=255)
                                       cTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTc                                                                                            <  2:1583553/139‑1 (MQ=255)
                                       cTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGC‑AAATTCGTCa                                                                                           <  1:1189978/139‑1 (MQ=255)
                                        tataACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCa                                                                                           <  2:1414827/139‑1 (MQ=255)
                                          taACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATg                                                                                         <  2:1312632/139‑1 (MQ=255)
                                             cccACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                                      <  1:409827/139‑1 (MQ=255)
                                                aCTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCGATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGttt                                                                                   >  1:545291/1‑139 (MQ=255)
                                                 cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAAt                                                                                                 <  2:1295270/124‑1 (MQ=255)
                                                 cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAAt                                                                                                 >  1:1295270/1‑124 (MQ=255)
                                                 cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                                                                  <  1:579898/139‑1 (MQ=255)
                                                 cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                                                                  <  1:895328/139‑1 (MQ=255)
                                                  tGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAg                                                                                 >  2:370966/1‑139 (MQ=255)
                                                    gTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGAc                                                                                                                             >  1:53786/1‑93 (MQ=255)
                                                    gTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGAc                                                                                                                             <  2:53786/93‑1 (MQ=255)
                                                         tCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTa                                                                          <  2:1342266/139‑1 (MQ=255)
                                                          cGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCa                                                                                           <  1:872289/121‑1 (MQ=255)
                                                          cGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCa                                                                                           >  2:872289/1‑121 (MQ=255)
                                                           gCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAgg                                                                        <  2:555058/139‑1 (MQ=255)
                                                            ccTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTTAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTAAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGc                                                                       >  1:1534898/1‑139 (MQ=255)
                                                             cTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTTAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCt                                                                      <  2:1534898/139‑1 (MQ=255)
                                                             cTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCt                                                                      <  2:1004754/139‑1 (MQ=255)
                                                              tGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCtt                                                                     >  1:1024693/1‑139 (MQ=255)
                                                               ggCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGtt                                                                           >  2:1112642/1‑132 (MQ=255)
                                                               ggCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGtt                                                                           <  1:1112642/132‑1 (MQ=255)
                                                               ggCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGtt                                                                           >  1:186656/1‑132 (MQ=255)
                                                               ggCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGtt                                                                           <  2:186656/132‑1 (MQ=255)
                                                                              aCCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGt                                                     <  2:1024693/139‑1 (MQ=255)
                                                                                               tCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGcc                                    >  2:950566/1‑139 (MQ=255)
                                                                                                 aaTGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                                      >  2:1586328/1‑87 (MQ=255)
                                                                                                 aaTGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                                      <  1:1586328/87‑1 (MQ=255)
                                                                                                    ggcgtggcgGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGAc                                                                                                                             >  2:203966/1‑45 (MQ=255)
                                                                                                    ggcgtggcgGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGAc                                                                                                                             <  1:203966/45‑1 (MQ=255)
                                                                                                    ggcgtggcgGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGTCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGt                                                     <  2:261457/117‑1 (MQ=255)
                                                                                                    ggcgtggcgGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGTCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGt                                                     >  1:261457/1‑117 (MQ=255)
                                                                                                    ggcgtggcgGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATgg                                                                                        <  1:1232311/82‑1 (MQ=255)
                                                                                                    ggcgtggcgGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATgg                                                                                        >  2:1232311/1‑82 (MQ=255)
                                                                                                           cggcAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCgg                        >  1:807130/1‑139 (MQ=255)
                                                                                                                    caggCGCAACCCTGGCGCTTGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgcg               <  1:199907/139‑1 (MQ=255)
                                                                                                                    caggCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgcg               <  1:791488/139‑1 (MQ=255)
                                                                                                                     aggCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgcgc              >  1:860039/1‑139 (MQ=255)
                                                                                                                         gcAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCa          <  2:807130/139‑1 (MQ=255)
                                                                                                                            acacTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGCTTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATgg       >  1:1092390/1‑139 (MQ=255)
                                                                                                                                 ggcgctggGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTg  >  2:176888/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                       
AGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCACTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTG  >  NC_000913/1458485‑1458752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑