Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	150,947	T→C	T3A (ACT→GCT)	yadC ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	150,947	0	T	C	100.0%	191.1 / NA	54	T3A (ACT→GCT)	yadC	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/27);  total (27/27)

TCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTT  >  NC_000913/150818‑151078                                                                                                                                  |                                                                                                                                    tCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAtttt                                                                                                                            >  1:1081097/1‑139 (MQ=255)        ggCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCa                                                                                                                     <  2:1081097/139‑1 (MQ=255)           aGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                                  >  1:1555522/1‑139 (MQ=255)                   ggCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                                          <  1:1161985/139‑1 (MQ=255)                    gCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTAt                                                                                                         >  1:813854/1‑139 (MQ=255)                                     cTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTcc                                                                                                                      >  2:1411492/1‑109 (MQ=255)                                     cTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTcc                                                                                                                      <  1:1411492/109‑1 (MQ=255)                                                tttCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTttgtttgt                                                                             >  1:1427579/1‑139 (MQ=255)                                                     cacaATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTc                                                                        >  1:1399569/1‑139 (MQ=255)                                                     cacaATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTc                                                                        <  2:1236067/139‑1 (MQ=255)                                                               cTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAata                                                              <  1:1354272/139‑1 (MQ=255)                                                                 tGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATc                                                            <  2:45324/139‑1 (MQ=255)                                                                  gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGtt                                                                                                     <  2:636238/97‑1 (MQ=255)                                                                  gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGtt                                                                                                     >  1:636238/1‑97 (MQ=255)                                                                            tgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                                                 <  2:813854/139‑1 (MQ=255)                                                                             gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAtt                                                                         <  1:1351445/114‑1 (MQ=255)                                                                             gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCg                                                                       >  2:411088/1‑116 (MQ=255)                                                                             gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCg                                                                       >  2:425934/1‑116 (MQ=255)                                                                             gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCg                                                                       <  1:411088/116‑1 (MQ=255)                                                                             gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCg                                                                       <  1:425934/116‑1 (MQ=255)                                                                             gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATg                                                >  1:683698/1‑139 (MQ=255)                                                                             gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTGTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAtt                                                                         >  2:1351445/1‑114 (MQ=255)                                                                              actgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                                          >  1:585024/1‑112 (MQ=255)                                                                              actgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                                          <  2:585024/112‑1 (MQ=255)                                                                                tgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                                          <  2:885309/78‑1 (MQ=255)                                                                                tgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                                          >  1:885309/1‑78 (MQ=255)                                                                                tgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAata                                                              <  2:620008/122‑1 (MQ=255)                                                                                tgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAata                                                              >  1:620008/1‑122 (MQ=255)                                                                                  tATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCa                                           >  2:1539983/1‑139 (MQ=255)                                                                                      acaacaAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAg                                       <  2:1555522/139‑1 (MQ=255)                                                                                        aacaaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGtt                                     >  2:1505765/1‑139 (MQ=255)                                                                                          caaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGa                                   <  2:1609396/139‑1 (MQ=255)                                                                                            aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 >  1:1210059/1‑139 (MQ=255)                                                                                            aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 <  1:1505765/139‑1 (MQ=255)                                                                                             gAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCa                                >  2:660960/1‑139 (MQ=255)                                                                                              aaTACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAg                               <  2:683698/139‑1 (MQ=255)                                                                                                tACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                                          <  1:329617/94‑1 (MQ=255)                                                                                                tACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                                          >  2:329617/1‑94 (MQ=255)                                                                                                     tGCTAAAAAAAGAATGTAGCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTATTATTCGTAGTAAATCTCCACTAACACATGGCTCATAAGTTGAcc                                 <  1:929487/130‑1 (MQ=255)                                                                                                     tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcg                                                                                           >  1:1378700/1‑72 (MQ=255)                                                                                                     tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcg                                                                                           <  2:1378700/72‑1 (MQ=255)                                                                                                     tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 >  2:929487/1‑130 (MQ=255)                                                                                                     tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAac                        >  2:366928/1‑139 (MQ=255)                                                                                                        tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCaa                                                                                                                    >  2:496689/1‑44 (MQ=255)                                                                                                        tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCaa                                                                                                                    <  1:496689/44‑1 (MQ=255)                                                                                                        tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACt                     <  2:1399569/139‑1 (MQ=255)                                                                                                          aaaaaaGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTtgtttgtt                                                                            >  2:161387/1‑82 (MQ=255)                                                                                                          aaaaaaGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTtgtttgtt                                                                            <  1:161387/82‑1 (MQ=255)                                                                                                                      aCCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGa       >  1:939156/1‑139 (MQ=255)                                                                                                                          gAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTTACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGAtatt   <  1:934422/139‑1 (MQ=255)                                                                                                                           aaGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGAttttt  >  2:538782/1‑139 (MQ=255)                                                                                                                             gATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                                                  <  2:571020/89‑1 (MQ=255)                                                                                                                             gATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                                                  >  1:571020/1‑89 (MQ=255)                                                                                                                               tAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAg                               <  1:617625/106‑1 (MQ=255)                                                                                                                                  |                                                                                                                                    TCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTT  >  NC_000913/150818‑151078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑