Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,559,680	G→A	G77G (GGC→GGT)	ddpC ←	D,D‑dipeptide ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,559,680	0	G	A	100.0%	151.6 / NA	47	G77G (GGC→GGT)	ddpC	D,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/26);  total (21/26)

AAGCACACCGGATAGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCT  >  NC_000913/1559548‑1559805                                                                                                                                     |                                                                                                                              aaGCACACCGGATAGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAAc                                                                                                                         <  2:1277542/139‑1 (MQ=255)              aGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAg                                                                                                            >  1:1293849/1‑139 (MQ=255)                aCATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGaa                                                                                                          >  2:101361/1‑139 (MQ=255)                  aTCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAgg                                                                                                        <  2:1535840/139‑1 (MQ=255)                      gAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAgg                                                                                                        >  1:1069819/1‑135 (MQ=255)                      gAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAgg                                                                                                        <  2:1069819/135‑1 (MQ=255)                            cGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAgg                                                                                              >  2:496301/1‑139 (MQ=255)                            cGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAgg                                                                                              >  2:736090/1‑139 (MQ=255)                              aaCCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcg                                                                                            >  2:872006/1‑139 (MQ=255)                                ccAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcggg                                                                                          <  1:496301/139‑1 (MQ=255)                                         cccGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAAt                                                                                 >  2:1572681/1‑139 (MQ=255)                                            gCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATTGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCaa                                                                              <  2:1117738/139‑1 (MQ=255)                                            gCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCaa                                                                              <  2:858345/139‑1 (MQ=255)                                                    aaCCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTg                                                                      >  1:1580259/1‑139 (MQ=255)                                                           aCTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCAt                                                               >  2:303669/1‑139 (MQ=255)                                                            cTAATCCGGCGAGAATTGATTGCTGACTGCTGACCAGTACGCGGCTAAACAGATCGCGTCCCACTGCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATg                                                              <  1:1554929/139‑1 (MQ=255)                                                            cTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATg                                                              <  2:1580259/139‑1 (MQ=255)                                                              aaTCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCg                                                            <  1:736090/139‑1 (MQ=255)                                                                    gCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCgcca                                                      >  1:1519853/1‑139 (MQ=255)                                                                       agaATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCgccagcc                                                   >  2:871525/1‑139 (MQ=255)                                                                         aaTTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCAc                                                 <  2:1293849/139‑1 (MQ=255)                                                                          attgattgCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACg                                                <  1:871525/139‑1 (MQ=255)                                                                                  cTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGaaaaa                                        >  1:1495852/1‑139 (MQ=255)                                                                                        gCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAatcatc                                  <  1:268935/139‑1 (MQ=255)                                                                                              cAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAAt                            <  1:101361/139‑1 (MQ=255)                                                                                              cAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAAt                            <  2:892111/139‑1 (MQ=255)                                                                                              cAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAAt                            <  1:792806/139‑1 (MQ=255)                                                                                                 tACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGc                         >  1:1132802/1‑139 (MQ=255)                                                                                                   cgcgGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCAt                       <  2:1132802/139‑1 (MQ=255)                                                                                                   cgcgGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCAt                       <  1:1572681/139‑1 (MQ=255)                                                                                                   cgcgGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCAt                       <  1:303669/139‑1 (MQ=255)                                                                                                      ggCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCgccagcca                                                  <  2:1304912/109‑1 (MQ=255)                                                                                                      ggCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCgccagcca                                                  >  1:1304912/1‑109 (MQ=255)                                                                                                       gCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAAt                   <  2:1273328/139‑1 (MQ=255)                                                                                                        cTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAAt                                                                             <  1:1419644/80‑1 (MQ=255)                                                                                                        cTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAAt                                                                             >  2:1419644/1‑80 (MQ=255)                                                                                                           aaCAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGt                                                                                      <  2:552920/68‑1 (MQ=255)                                                                                                           aaCAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGt                                                                                      >  1:552920/1‑68 (MQ=255)                                                                                                           aaCAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACaa               >  1:139145/1‑139 (MQ=255)                                                                                                                  cgcgTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGaaaaaa                                       >  2:1195899/1‑108 (MQ=255)                                                                                                                  cgcgTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGaaaaaa                                       <  1:1195899/108‑1 (MQ=255)                                                                                                                     gTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTgc     >  2:456235/1‑139 (MQ=255)                                                                                                                      tCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTgcg    <  2:1519853/139‑1 (MQ=255)                                                                                                                       cccACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTgcgc   <  1:872006/139‑1 (MQ=255)                                                                                                                        ccACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCt  >  1:837616/1‑139 (MQ=255)                                                                                                                              cATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCAt                       >  2:1413550/1‑112 (MQ=255)                                                                                                                              cATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCAt                       <  1:1413550/112‑1 (MQ=255)                                                                                                                                     |                                                                                                                              AAGCACACCGGATAGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCT  >  NC_000913/1559548‑1559805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑