Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	155,935	T→C	K89K (AAA→AAG)	yadV ←	putative periplasmic pilin chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	155,935	0	T	C	100.0%	232.5 / NA	64	K89K (AAA→AAG)	yadV	putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/34);  total (30/34)

AGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCA  >  NC_000913/155804‑156062                                                                                                                                    |                                                                                                                                agGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCAt                                                                                                                          <  1:366722/139‑1 (MQ=255) agGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCAt                                                                                                                          <  1:191400/139‑1 (MQ=255)   gCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGACTGTTTGCCCACGCTTGGCATCa                                                                                                                        <  1:1176117/139‑1 (MQ=255)    cTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCaa                                                                                                                       <  2:1316089/139‑1 (MQ=255)       tGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAc                                                                                                                    <  2:581480/139‑1 (MQ=255)        gATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcg                                                                                                                   <  1:535052/139‑1 (MQ=255)              cgACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTTTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCATGCTTGGCATCAATACGCGATAc                                                                                                             >  1:127678/1‑139 (MQ=255)                      tCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGgcggcg                                                                                                     <  2:715730/139‑1 (MQ=255)                         gCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                  <  1:1421045/139‑1 (MQ=255)                               ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaa                                                                                            >  2:142484/1‑139 (MQ=255)                               ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaa                                                                                            >  1:912945/1‑139 (MQ=255)                                   ttGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                        <  2:1401561/139‑1 (MQ=255)                                   ttGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                        <  1:295566/139‑1 (MQ=255)                                     ggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGc                                                                                                                            >  2:1300730/1‑101 (MQ=255)                                     ggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGc                                                                                                                            <  1:1300730/101‑1 (MQ=255)                                      gtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                  <  1:872257/126‑1 (MQ=255)                                      gtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                  >  2:872257/1‑126 (MQ=255)                                        ggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTg                                                                                   <  2:455910/139‑1 (MQ=255)                                          aaCTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaaa                                                                                          >  2:51723/1‑130 (MQ=255)                                          aaCTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaaa                                                                                          <  1:51723/130‑1 (MQ=255)                                          aaCTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                           >  1:1405660/1‑129 (MQ=255)                                          aaCTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                           <  2:1405660/129‑1 (MQ=255)                                                     cGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAgg                                                                      >  2:1111813/1‑139 (MQ=255)                                                      gTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGc                                                                     >  1:1054341/1‑139 (MQ=255)                                                        tAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTc                                                                   >  1:64293/1‑139 (MQ=255)                                                        tAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTc                                                                   >  1:676738/1‑139 (MQ=255)                                                             cAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTgg                                                                                                          >  1:840494/1‑95 (MQ=255)                                                             cAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTgg                                                                                                          <  2:840494/95‑1 (MQ=255)                                                               gAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTg                                                            >  1:1119112/1‑139 (MQ=255)                                                                aaCACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                        <  2:1359601/110‑1 (MQ=255)                                                                aaCACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                        >  1:1359601/1‑110 (MQ=255)                                                                 acacGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTc                                                          >  2:37259/1‑139 (MQ=255)                                                                  cacGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcg                                                                                                                   <  2:318747/81‑1 (MQ=255)                                                                  cacGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGCACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcg                                                                                                                   >  1:318747/1‑81 (MQ=255)                                                                   acGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCAt                                                        <  2:450842/139‑1 (MQ=255)                                                                            ctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTAt                                               <  1:142484/139‑1 (MQ=255)                                                                                   tAGGCAGTGAGGTGCTGGCTGCGTACATTAATTTGATTGTTTGCCCACGCTTgg                                                                                                                             <  2:1393871/54‑1 (MQ=255)                                                                                   tAGGCAGTGAGGTGCTGGCTGCGTACATTAATTTGATTGTTTGCCCACGCTTgg                                                                                                                             >  1:1393871/1‑54 (MQ=255)                                                                                     ggCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCa                                                                                                                        >  2:611172/1‑57 (MQ=255)                                                                                     ggCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCa                                                                                                                        <  1:611172/57‑1 (MQ=255)                                                                                       cAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAggg                                                                                       <  2:496403/88‑1 (MQ=255)                                                                                       cAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAggg                                                                                       >  1:496403/1‑88 (MQ=255)                                                                                         gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTg                                  >  1:1102863/1‑139 (MQ=255)                                                                                          tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATAc                                                                                                             <  2:1388642/63‑1 (MQ=255)                                                                                          tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATAc                                                                                                             >  1:1388642/1‑63 (MQ=255)                                                                                          tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                  <  2:322028/74‑1 (MQ=255)                                                                                          tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                  >  1:322028/1‑74 (MQ=255)                                                                                           gAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGa                                >  1:1436272/1‑139 (MQ=255)                                                                                                 ctggctgTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCa                          <  2:64293/139‑1 (MQ=255)                                                                                                    gctgTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACg                       >  2:1109417/1‑139 (MQ=255)                                                                                                      tgtgTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACt                                                                                                            >  2:406220/1‑52 (MQ=255)                                                                                                      tgtgTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACt                                                                                                            <  1:406220/52‑1 (MQ=255)                                                                                                        tgtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGtt                   <  2:615095/139‑1 (MQ=255)                                                                                                        tgtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGtt                   >  1:545037/1‑139 (MQ=255)                                                                                                          tACATTAATTTGATTGTTTGCCCATGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTAt                 <  2:127678/139‑1 (MQ=255)                                                                                                           aCATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTAtt                >  2:421940/1‑139 (MQ=255)                                                                                                              ttAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGt                                                                                                    <  2:570399/52‑1 (MQ=255)                                                                                                              ttAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGt                                                                                                    >  1:570399/1‑52 (MQ=255)                                                                                                               tAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCt            >  2:1606606/1‑139 (MQ=255)                                                                                                                    tGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAAc                        >  2:1167776/1‑122 (MQ=255)                                                                                                                    tGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAAc                        <  1:1167776/122‑1 (MQ=255)                                                                                                                       ttGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTc    <  1:1606606/139‑1 (MQ=255)                                                                                                                       ttGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTc    <  2:912945/139‑1 (MQ=255)                                                                                                                         gTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCa  <  1:37259/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                AGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCA  >  NC_000913/155804‑156062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑