Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,651,537 G→A pseudogene (693/693 nt) insD1 → IS2 transposase TnpB,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,651,5370GA100.0% 137.2 / NA 43pseudogene (693/693 nt)insD1IS2 transposase TnpB,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/21);  total (22/21)

GCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAAC  >  NC_000913/1651426‑1651666
                                                                                                               |                                                                                                                                 
gCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGctc                                                                                                        <  2:1319842/139‑1 (MQ=255)
    tataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCctct                                                                                                    >  2:1573515/1‑139 (MQ=255)
             tGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTAc                                                                                           <  2:1457329/139‑1 (MQ=255)
             tGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTAc                                                                                           >  1:1457329/1‑139 (MQ=255)
              ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACg                                                                                                           <  2:1530095/122‑1 (MQ=255)
              ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACg                                                                                                           >  1:1530095/1‑122 (MQ=255)
              ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACg                                                                                          >  1:1411742/1‑139 (MQ=255)
                cATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCa                                                                                        >  2:752226/1‑139 (MQ=255)
                                              ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAATTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTg                                                                              <  1:364405/119‑1 (MQ=255)
                                              ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTg                                                                              >  2:364405/1‑119 (MQ=255)
                                              ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTg                                                                              >  2:1410497/1‑119 (MQ=255)
                                              ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTg                                                                              <  1:1410497/119‑1 (MQ=255)
                                                            gCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCctcctc                                            >  1:174846/1‑139 (MQ=255)
                                                              ggCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGTAAATCCACAGGTGAACTACg                                                                                                           <  1:945945/74‑1 (MQ=255)
                                                              ggCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACg                                                                                                           >  2:945945/1‑74 (MQ=255)
                                                               gCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACg                                         <  2:1411742/139‑1 (MQ=255)
                                                               gCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACg                                         <  1:1342398/139‑1 (MQ=255)
                                                                   cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGa                                                                    <  2:777015/108‑1 (MQ=255)
                                                                   cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGa                                                                    >  1:777015/1‑108 (MQ=255)
                                                                                ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTc                                                   >  1:1465274/1‑112 (MQ=255)
                                                                                ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTc                                                   <  2:1465274/112‑1 (MQ=255)
                                                                                 gTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCaa                                                            <  2:694141/102‑1 (MQ=255)
                                                                                 gTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCaa                                                            >  1:694141/1‑102 (MQ=255)
                                                                                     aGTGATAACGGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCcctc                                               >  2:1082328/1‑111 (MQ=255)
                                                                                     aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTg                                                                              <  1:1214482/80‑1 (MQ=255)
                                                                                     aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTg                                                                              >  2:1214482/1‑80 (MQ=255)
                                                                                     aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGtt                                                     <  1:329172/105‑1 (MQ=255)
                                                                                     aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGtt                                                     >  2:329172/1‑105 (MQ=255)
                                                                                     aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCcctc                                               <  1:1082328/111‑1 (MQ=255)
                                                                                          tAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGct                                                                                                         >  1:18528/1‑48 (MQ=255)
                                                                                          tAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGcc                                                                                                         <  2:18528/48‑2 (MQ=18)
                                                                                          tAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTAc                                                                                           <  1:98016/62‑1 (MQ=255)
                                                                                          tAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTAc                                                                                           >  2:98016/1‑62 (MQ=255)
                                                                                          tAACAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCg              >  1:227744/1‑139 (MQ=255)
                                                                                           aaCAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGt                      <  2:472401/130‑1 (MQ=255)
                                                                                           aaCAGATGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGt                      >  1:472401/1‑130 (MQ=255)
                                                                                                 tGTCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGAt       >  2:21843/1‑139 (MQ=255)
                                                                                                   tCTGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGAttt     <  2:781051/139‑1 (MQ=255)
                                                                                                     tGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGa                    >  2:535414/1‑122 (MQ=255)
                                                                                                     tGGAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGa                    <  1:535414/122‑1 (MQ=255)
                                                                                                      ggAAATATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAAc  <  1:21843/139‑1 (MQ=255)
                                                                                                        aaaTATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGa             <  1:218117/126‑1 (MQ=255)
                                                                                                        aaaTATAAGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGa             >  2:218117/1‑126 (MQ=255)
                                                                                                               |                                                                                                                                 
GCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAAC  >  NC_000913/1651426‑1651666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: