Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,655,347 C→T *109* (TAG→TAA ynfA ← UPF0060 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,655,3470CT100.0% 110.4 / NA 35*109* (TAG→TAAynfAUPF0060 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/18);  total (17/18)

ATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACG  >  NC_000913/1655215‑1655479
                                                                                                                                    |                                                                                                                                    
aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTg                                                                                                                                <  1:862231/139‑1 (MQ=255)
     aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccc                                                                                                                           <  2:925364/139‑1 (MQ=255)
          gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                      <  1:545738/139‑5 (MQ=255)
                               ggCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGcc                                                                                                >  2:769283/1‑139 (MQ=255)
                                    atatTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCaaa                                                                                           >  1:1461504/1‑139 (MQ=255)
                                    atatTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCaaa                                                                                           >  1:308823/1‑139 (MQ=255)
                                           cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                    <  1:769283/139‑1 (MQ=255)
                                              tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                 <  2:308823/139‑1 (MQ=255)
                                              tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                 <  2:1461504/139‑1 (MQ=255)
                                                          aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                     >  1:1118643/1‑139 (MQ=255)
                                                                            tACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTg                                                                                                                                >  1:1297351/1‑63 (MQ=255)
                                                                            tACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTg                                                                                                                                <  2:1297351/63‑1 (MQ=255)
                                                                            tACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGttt                                                   >  2:208293/1‑139 (MQ=255)
                                                                               cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                      <  2:1448249/70‑5 (MQ=255)
                                                                               cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                      >  1:1448249/1‑66 (MQ=255)
                                                                                  ttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGcc                                             <  2:1118643/139‑1 (MQ=255)
                                                                                    gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAt                                           >  2:428791/1‑139 (MQ=255)
                                                                                         cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAgcgc                                                                                       <  2:602305/90‑1 (MQ=255)
                                                                                         cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAgcgc                                                                                       >  1:602305/1‑90 (MQ=255)
                                                                                         cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAgcgc                                                                                       <  2:1105680/89‑1 (MQ=255)
                                                                                         cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAgcgc                                                                                       >  1:1105680/1‑89 (MQ=255)
                                                                                            acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                      <  2:341608/57‑5 (MQ=255)
                                                                                            acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                      >  1:341608/1‑53 (MQ=255)
                                                                                                   ctctTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                       <  1:83926/96‑1 (MQ=255)
                                                                                                   ctctTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                       >  2:83926/1‑96 (MQ=255)
                                                                                                             atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAAt                                                                                                            >  1:527537/1‑49 (MQ=255)
                                                                                                             atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAAt                                                                                                            <  2:527537/49‑1 (MQ=255)
                                                                                                                   aaaaTGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGAt                                                                                                         <  2:961915/46‑1 (MQ=255)
                                                                                                                   aaaaTGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGAt                                                                                                         >  1:961915/1‑46 (MQ=255)
                                                                                                                           tttATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                 <  2:365487/126‑1 (MQ=255)
                                                                                                                           tttATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                 >  1:365487/1‑126 (MQ=255)
                                                                                                                           tttATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGCCATAAAGAGACAGTTTCACGCCATCCACAACGCGCAGCCACAACAACGCCGGGCAGCCATaaa    >  1:1524215/1‑139 (MQ=255)
                                                                                                                             tATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg  <  1:208293/139‑1 (MQ=255)
                                                                                                                               tGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                               >  2:3384/1‑76 (MQ=255)
                                                                                                                               tGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                               <  1:3384/76‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
ATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACG  >  NC_000913/1655215‑1655479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: