Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,670,581	G→A	G295R (GGA→AGA)	ynfM →	putative arabinose efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,670,581	0	G	A	100.0%	151.6 / NA	48	G295R (GGA→AGA)	ynfM	putative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/23);  total (25/23)

GTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCA  >  NC_000913/1670454‑1670709                                                                                                                                |                                                                                                                                 gTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGacc                                                                                                                       >  2:1186111/1‑139 (MQ=255)               tGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                         <  1:567365/139‑1 (MQ=255)                gTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGt                                                                                                        >  1:703092/1‑139 (MQ=255)                gTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGt                                                                                                        >  2:413596/1‑139 (MQ=255)                         cATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGaccacc                                                                                                                    <  1:1548077/118‑1 (MQ=255)                         cATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGaccacc                                                                                                                    >  2:1548077/1‑118 (MQ=255)                           tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCAt                                                                                                                           <  2:1597191/109‑1 (MQ=255)                           tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCAt                                                                                                                           >  1:1597191/1‑109 (MQ=255)                           tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTc                                                                                                    <  2:790629/132‑1 (MQ=255)                           tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTc                                                                                                    >  1:790629/1‑132 (MQ=255)                                            ctctCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACggggg                                                                            <  2:297246/139‑1 (MQ=255)                                               tcCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATGATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATCACCATCCGATATGGCCGTGGTCCAGCGATGTTGTTTTCGACGGGGGTTa                                                                         >  1:1588070/1‑139 (MQ=255)                                                    cTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTg                                                                    >  1:380990/1‑139 (MQ=255)                                                           gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                         <  2:62878/95‑1 (MQ=255)                                                           gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                         >  1:62878/1‑95 (MQ=255)                                                           gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGtt                                                             >  2:269970/1‑139 (MQ=255)                                                            tcagtcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGttt                                                            >  2:63104/1‑139 (MQ=255)                                                                 cagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                       <  1:63104/139‑1 (MQ=255)                                                                  agGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                         <  1:85004/88‑1 (MQ=255)                                                                  agGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                         >  2:85004/1‑88 (MQ=255)                                                                          gTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCCCCCACTATGGGCGTGCGCCAGTGATGTTGTTTTCGACGGGGGCTATGCTGTTTGGTTTACTGATGACCTTAt                                              >  1:186373/1‑139 (MQ=255)                                                                               cTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                         <  2:1297510/75‑1 (MQ=255)                                                                               cTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                         >  1:1297510/1‑75 (MQ=255)                                                                                 tattatCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTc                                       <  1:1186111/139‑1 (MQ=255)                                                                                    tatCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCt                                    <  1:413596/139‑1 (MQ=255)                                                                                        gCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTgg                                <  1:269970/139‑1 (MQ=255)                                                                                         cTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTTCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                       >  2:904215/1‑115 (MQ=255)                                                                                         cTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTTCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                       <  1:904215/115‑1 (MQ=255)                                                                                            gCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                       <  2:738206/112‑1 (MQ=255)                                                                                            gCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                       >  1:738206/1‑112 (MQ=255)                                                                                                      ccGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTa                                                                         >  1:412939/1‑84 (MQ=255)                                                                                                      ccGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTa                                                                         <  2:412939/84‑1 (MQ=255)                                                                                                          tACATGGAGCTCACCCAAAGCCAGAACCATGaccacc                                                                                                                    <  1:1419377/37‑1 (MQ=255)                                                                                                          tACATGGAGCTCACCCAAAGCCAGAACCATGaccacc                                                                                                                    >  2:1419377/1‑37 (MQ=255)                                                                                                            cATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGtt                                                                  >  2:423208/1‑85 (MQ=255)                                                                                                            cATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGtt                                                                  <  1:423208/85‑1 (MQ=255)                                                                                                               ggAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTAct         <  2:380990/139‑1 (MQ=255)                                                                                                                   cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                         >  1:654189/1‑39 (MQ=255)                                                                                                                   cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                         >  2:277660/1‑39 (MQ=255)                                                                                                                   cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                         <  1:277660/39‑1 (MQ=255)                                                                                                                   cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                         <  2:654189/39‑1 (MQ=255)                                                                                                                    tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGt                                                                                                <  2:207581/47‑1 (MQ=255)                                                                                                                    tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGt                                                                                                >  1:207581/1‑47 (MQ=255)                                                                                                                    tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGc                                     >  2:846776/1‑106 (MQ=255)                                                                                                                    tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGc                                     <  1:846776/106‑1 (MQ=255)                                                                                                                      aCCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTca  >  2:655828/1‑139 (MQ=255)                                                                                                                           aaGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCt                                        <  2:928391/96‑1 (MQ=255)                                                                                                                           aaGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCt                                        >  1:928391/1‑96 (MQ=255)                                                                                                                                |                                                                                                                                 GTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCA  >  NC_000913/1670454‑1670709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑