Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,575	G→A	intergenic (+14/+26)	cfa → / ← ribC	cyclopropane fatty acyl phospholipid synthase, SAM‑dependent/riboflavin synthase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,575	0	G	A	100.0%	180.2 / NA	57	intergenic (+14/+26)	cfa/ribC	cyclopropane fatty acyl phospholipid synthase, SAM‑dependent/riboflavin synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/29);  total (28/29)

GAATGTTTACCTATTATCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGAC  >  NC_000913/1742446‑1742705                                                                                                                                  |                                                                                                                                   gAATGTTTACCTATTATCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCtctc                                                                                                                           <  1:679079/139‑1 (MQ=255)             attatCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGAttt                                                                                                               <  1:1549121/139‑1 (MQ=255)              ttatCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTc                                                                                                              >  1:263715/1‑139 (MQ=255)                     aaTGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAg                                                                                                       >  2:225105/1‑139 (MQ=255)                     aaTGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAg                                                                                                       >  2:816396/1‑139 (MQ=255)                      aTGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCATGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCAcc                                                                                                                                  <  2:160316/111‑1 (MQ=255)                      aTGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCATGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCAcc                                                                                                                                  >  1:160316/1‑111 (MQ=255)                         ccTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCtt                                                                                                   <  1:37281/139‑1 (MQ=255)                         ccTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCtt                                                                                                   <  2:1246451/139‑1 (MQ=255)                           tgtgCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCCCGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCt                                                                                                 >  2:631468/1‑139 (MQ=255)                           tgtgCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCtt                                                                                                   >  1:161943/1‑137 (MQ=255)                           tgtgCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCtt                                                                                                   >  1:1516175/1‑137 (MQ=255)                           tgtgCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCtt                                                                                                   <  2:1516175/137‑1 (MQ=255)                           tgtgCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCtt                                                                                                   <  2:161943/137‑1 (MQ=255)                             tgCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCtgt                                                                                               >  2:840565/1‑139 (MQ=255)                               cAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGc                                                                                             <  2:875742/139‑1 (MQ=255)                                  gTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCAcccc                                                                                                                                <  1:250855/101‑1 (MQ=255)                                  gTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCAcccc                                                                                                                                >  2:250855/1‑101 (MQ=255)                                     cTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGtt                                                                                       >  2:473221/1‑139 (MQ=255)                                          cgcgCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTc                                                                                  <  1:840565/139‑1 (MQ=255)                                                cGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCa                                                                            >  1:1526658/1‑139 (MQ=255)                                                cGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCa                                                                            >  2:648905/1‑139 (MQ=255)                                                  tGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCAtt                                                                          >  1:495998/1‑139 (MQ=255)                                                            ctctGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCAcccc                                                                                                                                <  1:703691/75‑1 (MQ=255)                                                            ctctGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCAcccc                                                                                                                                >  2:703691/1‑75 (MQ=255)                                                            ctctGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgcc                                                                <  1:648905/139‑1 (MQ=255)                                                               tGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgccgcc                                                             >  2:560187/1‑139 (MQ=255)                                                                  cAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGc                                                          <  2:263715/139‑1 (MQ=255)                                                                   aGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGca                                                         <  1:360788/139‑1 (MQ=255)                                                                     gTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGt                                                                                        <  1:833851/106‑1 (MQ=255)                                                                     gTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGt                                                                                        >  2:833851/1‑106 (MQ=255)                                                                         tgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGtt                                                   <  1:473221/139‑1 (MQ=255)                                                                            tctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTa                                                <  2:495998/139‑1 (MQ=255)                                                                            tctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTa                                                <  1:225105/139‑1 (MQ=255)                                                                              tcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                              <  2:1526658/139‑1 (MQ=255)                                                                                aCGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCa                                                                                                        >  2:1243098/1‑79 (MQ=255)                                                                                aCGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCa                                                                                                        <  1:1243098/79‑1 (MQ=255)                                                                                aCGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCtt                                                                                                   >  2:1227941/1‑84 (MQ=255)                                                                                aCGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCtt                                                                                                   <  1:1227941/84‑1 (MQ=255)                                                                                     gtgtTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgccg                                                               >  1:297820/1‑115 (MQ=255)                                                                                     gtgtTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgccg                                                               <  2:297820/115‑1 (MQ=255)                                                                                     gtgtTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTa                                       >  1:39702/1‑139 (MQ=255)                                                                                        ttGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTTGTGCCGCCAGCACACGTTCTACCGTATCTACCa                                    >  2:925327/1‑139 (MQ=255)                                                                                             aaCGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGcc                               <  1:560187/139‑1 (MQ=255)                                                                                                ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCt                                                                                                 <  1:245811/70‑1 (MQ=255)                                                                                                ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCt                                                                                                 >  2:245811/1‑70 (MQ=255)                                                                                                ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGa                            >  1:669739/1‑139 (MQ=255)                                                                                                ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGa                            >  1:930549/1‑139 (MQ=255)                                                                                                          tGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGAt                  <  2:930549/139‑1 (MQ=255)                                                                                                            gCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCa                                                                            >  2:1058864/1‑79 (MQ=255)                                                                                                            gCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCa                                                                            <  1:1058864/79‑1 (MQ=255)                                                                                                                  tAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCa          <  1:1450125/139‑1 (MQ=255)                                                                                                                          aTTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGAc  >  2:72282/1‑139 (MQ=255)                                                                                                                            tCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGcac                                                        >  2:1094185/1‑83 (MQ=255)                                                                                                                            tCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGcac                                                        <  1:1094185/83‑1 (MQ=255)                                                                                                                            tCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGtt                                                   <  2:716465/88‑1 (MQ=255)                                                                                                                            tCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGtt                                                   >  1:716465/1‑88 (MQ=255)                                                                                                                                  |                                                                                                                                   GAATGTTTACCTATTATCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGAC  >  NC_000913/1742446‑1742705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑