Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,757,721 C→T *335* (TAG→TAA ldtE ← murein L,D‑transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,757,7210CT100.0% 151.7 / NA 47*335* (TAG→TAAldtEmurein L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/22);  total (25/22)

ATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGC  >  NC_000913/1757599‑1757855
                                                                                                                          |                                                                                                                                      
aTGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTa                                                                                                                        <  1:940984/139‑1 (MQ=255)
                                  caTGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgact                                                                                      >  2:979033‑M1/1‑120 (MQ=255)
                                       ctactaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTa                                                                                                                        <  2:269877/100‑1 (MQ=255)
                                       ctactaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTa                                                                                                                        >  1:269877/1‑100 (MQ=255)
                                               tACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgc                                                                         >  2:63562‑M1/1‑107 (MQ=255)
                                                    cAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGt                                                                                                                         >  2:877633/1‑86 (MQ=255)
                                                    cAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGt                                                                                                                         <  1:877633/86‑1 (MQ=255)
                                                    cAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgc                                                                    >  1:663520‑M1/1‑102 (MQ=255)
                                                       gTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg                                                                                                                          >  1:12082/1‑82 (MQ=255)
                                                       gTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg                                                                                                                          <  2:12082/82‑1 (MQ=255)
                                                            aGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTc                                                                                                                               <  2:205930/72‑1 (MQ=255)
                                                            aGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTc                                                                                                                               >  1:205930/1‑72 (MQ=255)
                                                              tACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctg                                                              <  1:856916‑M1/135‑44 (MQ=255)
                                                              tACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATGCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctg                                                              >  2:856916‑M1/1‑92 (MQ=255)
                                                               aCCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcggg                                                          <  1:656320‑M1/138‑48 (MQ=255)
                                                               aCCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcggg                                                          >  2:656320‑M1/1‑91 (MQ=255)
                                                                ccTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggag                                                        <  1:63562‑M1/139‑50 (MQ=255)
                                                                ccTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgact                                                                                      <  2:507645‑M1/109‑20 (MQ=255)
                                                                ccTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgact                                                                                      >  1:507645‑M1/1‑90 (MQ=255)
                                                                 cTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagt                                                       >  1:1031872‑M1/1‑89 (MQ=255)
                                                                 cTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                                          >  1:1091944‑M1/1‑89 (MQ=255)
                                                                 cTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                                          <  2:1091944‑M1/104‑16 (MQ=255)
                                                                   gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgc                                                                                                                           <  2:1260341/69‑1 (MQ=255)
                                                                   gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgc                                                                                                                           >  1:1260341/1‑69 (MQ=255)
                                                                   gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTcc                                                                                                               >  2:219970/1‑81 (MQ=255)
                                                                   gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTcc                                                                                                               <  1:219970/81‑1 (MQ=255)
                                                                   gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                                          <  1:200977‑M1/102‑16 (MQ=255)
                                                                   gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                                          >  2:200977‑M1/1‑87 (MQ=255)
                                                                     gaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTg                                                                                                           >  1:1598294/1‑83 (MQ=255)
                                                                     gaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTg                                                                                                           <  2:1598294/83‑1 (MQ=255)
                                                                        gtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctcca                                                >  1:1116601‑M1/1‑82 (MQ=255)
                                                                                   cgcACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccga                                     <  1:263582‑M1/139‑69 (MQ=255)
                                                                                   cgcACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactg                                                                                <  2:1014861‑M1/96‑26 (MQ=255)
                                                                                   cgcACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactg                                                                                >  1:1014861‑M1/1‑71 (MQ=255)
                                                                                     cacaTTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGt                                                                                                                         <  2:1147958/53‑1 (MQ=255)
                                                                                     cacaTTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGt                                                                                                                         >  1:1147958/1‑53 (MQ=255)
                                                                                            tgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggata                            >  1:1462208‑M1/1‑62 (MQ=255)
                                                                                            tgCGCCATTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggatac                           >  2:1377481‑M1/1‑61 (MQ=255)
                                                                                              cgcCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctgg                                                                 >  1:211486‑M1/1‑60 (MQ=255)
                                                                                              cgcCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctgg                                                                 <  2:211486‑M1/100‑41 (MQ=255)
                                                                                              cgcCATTTTTTTTGTCTGCCGTTTACCGTTACCGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggag                                                        >  2:723993‑M1/1‑60 (MQ=255)
                                                                                              cgcCATTTTTTTTGTCTGCCGTTTACCGTTACCGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggag                                                        <  1:723993‑M1/109‑50 (MQ=255)
                                                                                                  aTTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcg                                                                                            <  2:1088863‑M1/69‑14 (MQ=255)
                                                                                                  aTTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcg                                                                                            >  1:1088863‑M1/1‑56 (MQ=255)
                                                                                                    tttttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgccc                    >  1:1219538‑M1/1‑54 (MQ=255)
                                                                                                                      aCCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgcccgacgatacaacgctttat  <  2:1462208‑M1/139‑104 (MQ=255)
                                                                                                                      aCCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgcccgacgatacaacgctttat  <  1:1377481‑M1/139‑104 (MQ=255)
                                                                                                                          |                                                                                                                                      
ATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGC  >  NC_000913/1757599‑1757855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: