Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,781,964	C→G	G190G (GGC→GGG)	ydiS →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,781,964	0	C	G	100.0%	224.6 / NA	62	G190G (GGC→GGG)	ydiS	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (34/28);  total (34/28)

AGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCATATCC  >  NC_000913/1781828‑1782096                                                                                                                                         |                                                                                                                                       aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                                      <  2:1422998/139‑1 (MQ=255) aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                                      <  1:723291/139‑1 (MQ=255) aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                                      <  1:1518661/139‑1 (MQ=255)   gCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑ca                                                                                                                                  >  2:26783/1‑139 (MQ=255)      ggggATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑Cacac                                                                                                                               >  1:202894/1‑139 (MQ=255)        ggATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCa                                                                                                                             >  2:236946/1‑139 (MQ=255)             atatTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACa                                                                                                                        >  1:533640/1‑139 (MQ=255)             atatTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACa                                                                                                                        >  2:862986/1‑139 (MQ=255)             atatTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACa                                                                                                                        >  2:728569/1‑139 (MQ=255)                      aaGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGa                                                                                                               >  2:815290/1‑139 (MQ=255)                       aGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGAt                                                                                                              <  1:728569/139‑1 (MQ=255)                       aGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGAt                                                                                                              <  1:26783/139‑1 (MQ=255)                       aGCGAATGTGGTGATTCTGGCTCATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGAt                                                                                                              >  1:1469935/1‑139 (MQ=255)                             tgtgGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                                      >  2:650822/1‑111 (MQ=255)                             tgtgGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCttt                                                                                                        <  1:862986/139‑1 (MQ=255)                               tggtgATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGAt                                                                                                              <  2:1510221/131‑1 (MQ=255)                               tggtgATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGAt                                                                                                              >  1:1510221/1‑131 (MQ=255)                                   gATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                                      >  1:924764/1‑105 (MQ=255)                                      tCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGaa                                                                                                                          <  1:1549547/112‑1 (MQ=255)                                      tCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGaa                                                                                                                          >  2:1549547/1‑112 (MQ=255)                                       cTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACgg                                                                                              <  1:236946/139‑1 (MQ=255)                                       cTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACgg                                                                                              >  1:913979/1‑139 (MQ=255)                                        tGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACACCAGAACAGATCAACGATCGCTTTaa                                                                                                      <  1:897077/132‑1 (MQ=255)                                        tGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACACCAGAACAGATCAACGATCGCTTTaa                                                                                                      >  2:897077/1‑132 (MQ=255)                                               tGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGaa                                                                                      >  2:613354/1‑139 (MQ=255)                                                ggCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                                      >  2:1315001/1‑92 (MQ=255)                                                    ttAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGAt                                                                                                              >  1:1040533/1‑110 (MQ=255)                                                    ttAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGAt                                                                                                              <  2:1040533/110‑1 (MQ=255)                                                         tCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCt                                                                            <  1:614015/139‑1 (MQ=255)                                                           gATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTgg                                                                          <  1:613354/139‑1 (MQ=255)                                                            aTGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGc                                                                                            <  2:1370871/120‑1 (MQ=255)                                                            aTGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGc                                                                                            >  1:1370871/1‑120 (MQ=255)                                                            aTGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGc                                                                         >  1:739165/1‑139 (MQ=255)                                                                 tGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGttt                                                                    <  2:739165/139‑1 (MQ=255)                                                                   gCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGc                                                                  >  1:1389938/1‑139 (MQ=255)                                                                        tcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGtt                                                             <  2:1333271/139‑1 (MQ=255)                                                                                 aTGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTg                                                    <  2:1389938/139‑1 (MQ=255)                                                                                   ggTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGAc                                                  <  1:815290/139‑1 (MQ=255)                                                                                    gTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACg                                                 >  1:128361/1‑139 (MQ=255)                                                                                        ccGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                                      >  2:1418214/1‑52 (MQ=255)                                                                                        ccGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                                      <  1:1418214/52‑1 (MQ=255)                                                                                          gCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGa                                           <  2:202894/139‑1 (MQ=255)                                                                                               cGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGc                                      >  1:1399700/1‑139 (MQ=255)                                                                                                 aTCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCgg                                    >  1:1207356/1‑139 (MQ=255)                                                                                                     gCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGa                                <  2:1469935/139‑1 (MQ=255)                                                                                                      catcatTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                                      >  2:968275/1‑38 (MQ=255)                                                                                                      catcatTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                                      >  1:1529125/1‑38 (MQ=255)                                                                                                      catcatTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAt                               <  2:1399700/139‑1 (MQ=255)                                                                                                      catcatTACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAt                               <  2:1207356/139‑1 (MQ=255)                                                                                                            tACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTg                                                    >  2:266599/1‑112 (MQ=255)                                                                                                            tACGCTGTTGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTg                                                    <  1:266599/112‑1 (MQ=255)                                                                                                                   ttGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaa                  >  2:1188383/1‑139 (MQ=255)                                                                                                                   ttGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaa                  >  2:516626/1‑139 (MQ=255)                                                                                                                   ttGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaa                  >  1:270136/1‑139 (MQ=255)                                                                                                                    tGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTgg                                                                          >  2:563164/1‑82 (MQ=255)                                                                                                                    tGGTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTgg                                                                          <  1:563164/82‑1 (MQ=255)                                                                                                                     ggTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTa                                                                                                 >  1:1529243/1‑58 (MQ=255)                                                                                                                     ggTGTTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTa                                                                                                 <  2:1529243/58‑1 (MQ=255)                                                                                                                        gtTAAAGAGGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAgg             <  1:516626/139‑1 (MQ=255)                                                                                                                             agagGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAt                               <  1:55038/116‑1 (MQ=255)                                                                                                                             agagGTTATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAt                               >  2:55038/1‑116 (MQ=255)                                                                                                                                   tATTGGGCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCATATcc  >  1:479265/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                       AGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCT‑‑CACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCATATCC  >  NC_000913/1781828‑1782096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑