Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,792,267	C→T	*155* (TAG→TAA)	nlpC ←	putative C40 clan peptidase lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,792,267	0	C	T	100.0%	93.0 / NA	30	*155* (TAG→TAA)	nlpC	putative C40 clan peptidase lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

TCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGT  >  NC_000913/1792158‑1792367                                                                                                              |                                                                                                     tCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAAttttt                                                                         >  2:877579/1‑139 (MQ=255)  ccTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTc                                                                        >  1:610170/1‑139 (MQ=255)   cTGATTATAAACCAGTAATCTATTTACGGAATTTTTGTTTGGAAAATACTACTGTATTCAGGAGTAAATGGATGGCGATGGAACATCGATGTCAATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCt                                                                       <  1:1063143/139‑1 (MQ=255)           aaaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAg                                                               <  1:877579/139‑1 (MQ=255)                aataatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACAtt                                                          <  2:610170/139‑1 (MQ=255)                          ttACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAg                                                >  1:707322/1‑139 (MQ=255)                                  tttttGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCAt                                        <  2:707322/139‑1 (MQ=255)                                                         tATTCAGGAGTAAATGGACGGCGATGTTTCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAg                                                >  2:181449/1‑108 (MQ=255)                                                         tATTCAGGAGTAAATGGACGGCGATGTTTCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAg                                                <  1:181449/108‑1 (MQ=255)                                                         tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGc                                                                     <  1:1341063/87‑1 (MQ=255)                                                         tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGc                                                                     >  2:1341063/1‑87 (MQ=255)                                                         tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACAtt                                                          >  2:1018555/1‑98 (MQ=255)                                                         tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACAtt                                                          <  1:1018555/98‑1 (MQ=255)                                                         tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGt                 >  2:910399/1‑139 (MQ=255)                                                              aGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCt                                                                       >  1:1253221/1‑80 (MQ=255)                                                              aGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCt                                                                       <  2:1253221/80‑1 (MQ=255)                                                                   tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAAc                                            <  1:282587/102‑1 (MQ=255)                                                                   tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAAc                                            >  2:282587/1‑102 (MQ=255)                                                                     aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAg                                                    <  2:28114/92‑1 (MQ=255)                                                                     aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAg                                                    >  1:28114/1‑92 (MQ=255)                                                                     aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACt                                    >  2:248853/1‑108 (MQ=255)                                                                     aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACt                                    <  1:248853/108‑1 (MQ=255)                                                                        ggACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGa                                                                                                  <  2:1494394/43‑1 (MQ=255)                                                                        ggACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGa                                                                                                  >  1:1494394/1‑43 (MQ=255)                                                                                           ccGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGTCAATAg                                                               >  2:152637/1‑59 (MQ=255)                                                                                           ccGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGTCAATAg                                                               <  1:152637/59‑1 (MQ=255)                                                                                           ccGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgttgt  <  2:581556/120‑1 (MQ=255)                                                                                           ccGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgttgt  >  1:581556/1‑120 (MQ=255)                                                                                             gTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTg       >  2:173379/1‑113 (MQ=255)                                                                                             gTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTg       <  1:173379/113‑1 (MQ=255)                                                                                                              |                                                                                                     TCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGT  >  NC_000913/1792158‑1792367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑