Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,809,787 T→C D136D (GAT→GAC)  yniC → 2‑deoxyglucose‑6‑P phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,809,7870TC100.0% 189.4 / NA 53D136D (GAT→GAC) yniC2‑deoxyglucose‑6‑P phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/26);  total (27/26)

CGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGATGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCATGATC  >  NC_000913/1809667‑1809916
                                                                                                                        |                                                                                                                                 
cGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGaa                                                                                                                 >  2:124607/1‑139 (MQ=255)
  tGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGaaaa                                                                                                               >  1:659563/1‑139 (MQ=255)
   gcgcGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACGACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCACGCCTCGGCCGaaaaa                                                                                                              <  2:1196041/139‑1 (MQ=255)
                     aTGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCg                                                                                            >  1:1293744/1‑139 (MQ=255)
                           aGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGaaa                                                                                                                <  1:1235207/113‑1 (MQ=255)
                           aGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGaaa                                                                                                                >  2:1235207/1‑113 (MQ=255)
                           aGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCg                                                                                      <  2:659563/139‑1 (MQ=255)
                               cAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAg                                                                                  <  2:1564264/139‑1 (MQ=255)
                                aaGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGt                                                                                 >  2:374486/1‑139 (MQ=255)
                                          ggtggGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCg                                                                                      <  2:1336286/124‑1 (MQ=255)
                                          ggtggGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCg                                                                                      >  1:1336286/1‑124 (MQ=255)
                                            tggGACTGGCCTCCGCGTCACCATTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTg                                                                     <  2:674327/139‑1 (MQ=255)
                                             gggACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGc                                                                                           <  2:1463460/116‑1 (MQ=255)
                                             gggACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGc                                                                                           >  1:1463460/1‑116 (MQ=255)
                                              ggACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTgcg                                                                   >  1:1188932/1‑139 (MQ=255)
                                                   ggCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCgcagca                                                              >  2:474287/1‑139 (MQ=255)
                                                         cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcc                                                                                                                          <  1:84208/73‑1 (MQ=255)
                                                         cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcc                                                                                                                          >  1:1357027/1‑73 (MQ=255)
                                                         cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcc                                                                                                                          <  2:1357027/73‑1 (MQ=255)
                                                         cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcc                                                                                                                          >  2:84208/1‑73 (MQ=255)
                                                         cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATcc                                                                                       <  1:637554/108‑1 (MQ=255)
                                                         cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATcc                                                                                       >  2:637554/1‑108 (MQ=255)
                                                           cgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTggg                                                      <  1:474287/139‑1 (MQ=255)
                                                              caccacTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGt                                                   >  2:125311/1‑139 (MQ=255)
                                                              caccacTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGt                                                   >  1:805537/1‑139 (MQ=255)
                                                                   cTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcct                                                                                                                         <  1:1228729/64‑1 (MQ=255)
                                                                   cTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcct                                                                                                                         >  2:1228729/1‑64 (MQ=255)
                                                                       atatGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCctct                                          <  1:124607/139‑1 (MQ=255)
                                                                          tGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGAc                                       <  1:374486/139‑1 (MQ=255)
                                                                           gCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCg                                                                                            <  1:735478/85‑1 (MQ=255)
                                                                           gCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCg                                                                                            >  2:735478/1‑85 (MQ=255)
                                                                           gCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGAcc                                      >  1:1292989/1‑139 (MQ=255)
                                                                           gCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGAc                                       >  2:1305618/1‑138 (MQ=255)
                                                                           gCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGAc                                       <  1:1305618/138‑1 (MQ=255)
                                                                              ggAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcctc                                                                                                                        >  2:696501/1‑54 (MQ=255)
                                                                              ggAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcctc                                                                                                                        <  1:696501/54‑1 (MQ=255)
                                                                              ggAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGc                                   >  1:84012/1‑139 (MQ=255)
                                                                                     gtgtTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTg                                                 <  2:1276413/118‑1 (MQ=255)
                                                                                     gtgtTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTg                                                 >  1:1276413/1‑118 (MQ=255)
                                                                                                gTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAg                                                                                  <  2:1186994/74‑1 (MQ=255)
                                                                                                gTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAg                                                                                  >  1:1186994/1‑74 (MQ=255)
                                                                                                     aCTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCgcagca                                                              >  1:1475978/1‑89 (MQ=255)
                                                                                                     aCTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCgcagca                                                              <  2:1475978/89‑1 (MQ=255)
                                                                                                          cgcgATAGTTTCGACGCCCTCGCCTCGGCCGaaaa                                                                                                               <  1:930796/35‑1 (MQ=255)
                                                                                                          cgcgACAGTTTCGACGCCCTCGCCTCGGCCGaaaa                                                                                                               >  2:930796/1‑35 (MQ=255)
                                                                                                          cgcgACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATcc                                                                                       <  2:1214442/59‑1 (MQ=255)
                                                                                                          cgcgACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATcc                                                                                       >  1:1214442/1‑59 (MQ=255)
                                                                                                          cgcgACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCa       >  1:1455228/1‑139 (MQ=255)
                                                                                                               cAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCATGATc  <  2:1292989/139‑1 (MQ=255)
                                                                                                               cAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCATGATc  <  2:1188932/139‑1 (MQ=255)
                                                                                                               cAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCATGATc  <  2:84012/139‑1 (MQ=255)
                                                                                                                    tCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAg                                                                                  <  2:1126554/54‑1 (MQ=255)
                                                                                                                    tCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAg                                                                                  >  1:1126554/1‑54 (MQ=255)
                                                                                                                        |                                                                                                                                 
CGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGATGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCATGATC  >  NC_000913/1809667‑1809916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑