Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,892,402	A→G	S278S (AGT→AGC)	yoaA ←	putative ATP‑dependent helicase, DinG family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,892,402	0	A	G	100.0%	124.0 / NA	35	S278S (AGT→AGC)	yoaA	putative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/19);  total (16/19)

GTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCA  >  NC_000913/1892266‑1892537                                                                                                                                         |                                                                                                                                        gTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGct                                                                                                                                       <  1:433503/139‑1 (MQ=255)       ccACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGc                                                                                                                                 <  2:84393/139‑1 (MQ=255)                     aGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                                   >  1:1021176/1‑139 (MQ=255)                     aGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                                   <  2:756010/139‑1 (MQ=255)                       ttCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                                                    <  1:124545/136‑1 (MQ=255)                       ttCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                                                    >  2:124545/1‑136 (MQ=255)                             ggTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGt                                                                                                           >  2:1479091/1‑139 (MQ=255)                                 tCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAAct                                                                                                       >  2:1597049/1‑139 (MQ=255)                                       aGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTggg                                                                                                 <  2:169959/139‑1 (MQ=255)                                        gCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgt                                                                                                <  1:1479091/139‑1 (MQ=255)                                        gCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgt                                                                                                <  2:1323669/139‑1 (MQ=255)                                         cAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgtg                                                                                               >  2:318833/1‑139 (MQ=255)                                                      cGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCgg                                                                                  >  1:1020894/1‑139 (MQ=255)                                                        cTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTg                                                                                <  2:1020894/139‑1 (MQ=255)                                                             tttGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTa                                                                           <  1:1597049/139‑1 (MQ=255)                                                                        gCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGa                                                                <  2:719873/139‑1 (MQ=255)                                                                            aCAGCTCACGCAGGTTACCGCGATAACCTGGCTCACGGAGTTGCAGACGACAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTc                                                            <  2:1021176/139‑1 (MQ=255)                                                                                  cACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCGCTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCg                                                                       >  1:69725/1‑122 (MQ=255)                                                                                  cACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCg                                                                       <  2:69725/122‑1 (MQ=255)                                                                                   aCGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGcc                                                     <  2:519441/139‑1 (MQ=255)                                                                                         gTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCg                                               >  1:1326123/1‑139 (MQ=255)                                                                                         gTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCg                                               <  1:318833/139‑1 (MQ=255)                                                                                           tACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAg                                             <  2:1326123/139‑1 (MQ=255)                                                                                               gcgATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                                   <  1:812292/65‑1 (MQ=255)                                                                                               gcgATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                                   >  2:812292/1‑65 (MQ=255)                                                                                                   tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTc                                     >  2:1168504/1‑139 (MQ=255)                                                                                                   tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTc                                     >  2:1104145/1‑139 (MQ=255)                                                                                                   tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTc                                     >  1:210934/1‑139 (MQ=255)                                                                                                           cTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGt                                                                                                           >  2:310290/1‑61 (MQ=255)                                                                                                           cTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGt                                                                                                           <  1:310290/61‑1 (MQ=255)                                                                                                               ccGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGa                                   >  1:73393/1‑129 (MQ=255)                                                                                                               ccGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGa                                   <  2:73393/129‑1 (MQ=255)                                                                                                               ccGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtg                         >  1:849787/1‑139 (MQ=255)                                                                                                                  aGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtgact                      <  1:535592/139‑1 (MQ=255)                                                                                                                    ttGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGGGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtgactga                    >  1:1052707/1‑139 (MQ=255)                                                                                                                                      cgcgCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCa  <  1:1168504/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCA  >  NC_000913/1892266‑1892537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑