Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,016,095 G→A L76L (CTG→CTA)  fliH → negative regulator of FliI ATPase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,016,0950GA100.0% 181.2 / NA 56L76L (CTG→CTA) fliHnegative regulator of FliI ATPase activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/27);  total (29/27)

GAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAGCGTCGCGCCTGAT  >  NC_000913/2015967‑2016229
                                                                                                                                |                                                                                                                                      
gAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGc                                                                                                                              <  1:1405394/139‑1 (MQ=255)
  agagGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTg                                                                                                                            <  2:1467361/139‑1 (MQ=255)
   gagGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATCGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTgg                                                                                                                           >  1:1022233/1‑139 (MQ=255)
      gCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCTCATGAGCAAGGTTATGAGGCGGGTATTGCCGAAGGTCGCAAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGc                                                                                                                        >  2:1027104/1‑139 (MQ=255)
      gCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGc                                                                                                                        >  1:295285/1‑139 (MQ=255)
      gCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGc                                                                                                                        <  1:1469487/139‑1 (MQ=255)
       cTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATAAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCa                                                                                                                       <  2:1022233/139‑1 (MQ=255)
                       gcagcaACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCa                                                                                                                       <  1:1412773/123‑1 (MQ=255)
                       gcagcaACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCa                                                                                                                       >  2:1412773/1‑123 (MQ=255)
                           caACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGt                                                                                                   >  1:1334954/1‑139 (MQ=255)
                             aCTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGtct                                                                                                 <  1:433814/139‑1 (MQ=255)
                                      aCTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGc                                                                                                                              >  1:730686/1‑101 (MQ=255)
                                      aCTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGc                                                                                                                              <  2:730686/101‑1 (MQ=255)
                                       cTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGc                                                                                                        >  2:382054/1‑122 (MQ=255)
                                       cTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGc                                                                                                        <  1:382054/122‑1 (MQ=255)
                                                    cccATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCg                                                                          >  2:544463/1‑139 (MQ=255)
                                                    cccATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCg                                                                          <  2:295285/139‑1 (MQ=255)
                                                        tGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATg                                                                      >  2:1581061/1‑139 (MQ=255)
                                                              aGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCaa                                                                <  2:1334954/139‑1 (MQ=255)
                                                               ggTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAAc                                                               >  1:172631/1‑139 (MQ=255)
                                                                    tCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTc                                                          >  2:895189/1‑139 (MQ=255)
                                                                     cAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCa                                                         >  2:793887/1‑139 (MQ=255)
                                                                          gggTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCa                                                                                                                                   <  1:128704/60‑1 (MQ=255)
                                                                          gggTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCa                                                                                                                                   >  2:128704/1‑60 (MQ=255)
                                                                          gggTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGaa                                                    >  2:219208/1‑139 (MQ=255)
                                                                                  ccGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAAc                                            <  1:1581061/139‑1 (MQ=255)
                                                                                   cGAAGGTCGCCAGCAAGGTCATAAGCGGGGCTATCAGCAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACt                                           <  1:1027104/139‑1 (MQ=255)
                                                                                       ggTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTAccc                                       >  1:182087/1‑139 (MQ=255)
                                                                                          cGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTg                                    >  2:751382/1‑139 (MQ=255)
                                                                                             cAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATg                                 <  1:793887/139‑1 (MQ=255)
                                                                                              aGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGc                                <  2:920235/139‑1 (MQ=255)
                                                                                              aGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGc                                <  1:544463/139‑1 (MQ=255)
                                                                                                  aGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGACTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACtt                            >  1:396362/1‑139 (MQ=255)
                                                                                                    gTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGa                          <  2:182087/139‑1 (MQ=255)
                                                                                                       aTAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGc                                                                                                                              >  2:368673/1‑36 (MQ=255)
                                                                                                       aTAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGc                                                                                                                              <  1:368673/36‑1 (MQ=255)
                                                                                                       aTAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGc                                                                                                        <  2:947699/58‑1 (MQ=255)
                                                                                                       aTAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGc                                                                             >  1:484244/1‑85 (MQ=255)
                                                                                                       aTAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGc                                                                             <  2:484244/85‑1 (MQ=255)
                                                                                                       aTAAGCAGGGCTATCAGGAACGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGc                                                                                                        >  1:947699/1‑58 (MQ=255)
                                                                                                             aGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGAt                 >  1:597119/1‑139 (MQ=255)
                                                                                                               ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCg                                                                                                       >  1:507570/1‑51 (MQ=255)
                                                                                                               ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCg                                                                                                       >  1:681863/1‑51 (MQ=255)
                                                                                                               ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCg                                                                                                       <  2:681863/51‑1 (MQ=255)
                                                                                                               ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCg                                                                                                       <  2:507570/51‑1 (MQ=255)
                                                                                                               ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGTCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTa                                          >  1:1156324/1‑112 (MQ=255)
                                                                                                               ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGaa                                                    <  2:388420/102‑1 (MQ=255)
                                                                                                               ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGaa                                                    >  1:388420/1‑102 (MQ=255)
                                                                                                               ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTa                                          <  2:1156324/112‑1 (MQ=255)
                                                                                                               ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGa                          <  1:1405413/128‑1 (MQ=255)
                                                                                                               ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGa                          >  2:1405413/1‑128 (MQ=255)
                                                                                                               ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAg               >  2:668850/1‑139 (MQ=255)
                                                                                                               ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAg               >  2:1096872/1‑139 (MQ=255)
                                                                                                                 cTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAGCg             >  1:253377/1‑139 (MQ=255)
                                                                                                                   aTCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAGCGTc           <  2:253377/139‑1 (MQ=255)
                                                                                                                            gaCTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAGCGTCGCGCCTGAt  <  2:597119/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                      
GAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAGCGTCGCGCCTGAT  >  NC_000913/2015967‑2016229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑