| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,068,952 | C→T | *302* (TAG→TAA) | insD1 ← | IS2 transposase TnpB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,068,952 | 0 | C | T | 98.2% | 177.6 / ‑4.6 | 57 | *302* (TAG→TAA) | insD1 | IS2 transposase TnpB |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (28/28); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ATTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCT > NC_000913/2068818‑2069075 | aTTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTtata > 2:217682/1‑139 (MQ=255) tGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAtt > 1:990623/1‑139 (MQ=255) tGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAtt > 2:1592702/1‑139 (MQ=255) aacaaacaTTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAg < 1:1592702/139‑1 (MQ=255) tGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGtt > 2:248566/1‑139 (MQ=255) gCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTa > 1:148814/1‑139 (MQ=255) gCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTa > 1:231312/1‑139 (MQ=255) caccaATGCCTCCATAGCCGATAATTTGCAGGACAACCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAc > 1:19858/1‑139 (MQ=255) gCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc < 1:418663/133‑1 (MQ=255) gCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc > 2:418663/1‑133 (MQ=255) gCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTAc > 2:1563904/1‑139 (MQ=255) tAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg < 2:243927/139‑1 (MQ=255) gATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc < 1:1563904/139‑1 (MQ=255) aTAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg > 1:1207970/1‑139 (MQ=255) aTAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATCTCCAGACAt < 2:726490/104‑1 (MQ=255) aTAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATCTCCAGACAt > 1:726490/1‑104 (MQ=255) tAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGACTGGATTTGCCCTTATATTTCCAGACATCTGTTATCGCTTAACCCATTACAAGCCCGCTGCCGc > 1:1468410/1‑139 (MQ=255) tCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTgg < 2:1207970/139‑1 (MQ=255) ccccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGa < 1:234474/85‑1 (MQ=255) ccccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGa > 2:234474/1‑85 (MQ=255) cccGGAGTAGAGATAACTGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATCTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgct < 2:990623/139‑2 (MQ=255) gAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt > 1:477839/1‑83 (MQ=255) gAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt < 2:477839/83‑1 (MQ=255) gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt > 1:849314/1‑78 (MQ=255) gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt < 2:849314/78‑1 (MQ=255) gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg < 1:875716/113‑1 (MQ=255) gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg > 2:875716/1‑113 (MQ=255) aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg > 1:922010/1‑139 (MQ=255) aGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt > 2:286744/1‑81 (MQ=255) aGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt < 1:286744/81‑1 (MQ=255) aGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCa < 2:148814/139‑1 (MQ=255) aGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCa < 2:231312/139‑1 (MQ=255) aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTtat < 1:441516/56‑1 (MQ=255) aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTtat > 2:441516/1‑56 (MQ=255) aCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc < 1:1448767/99‑1 (MQ=255) aCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc > 2:1448767/1‑99 (MQ=255) cTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGa < 1:248566/139‑1 (MQ=255) ttGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACtt < 2:933829/72‑1 (MQ=255) ttGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACtt > 1:933829/1‑72 (MQ=255) gCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc < 2:19858/139‑1 (MQ=255) ggCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAtt < 1:336162/139‑1 (MQ=255) gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg > 1:1446707/1‑120 (MQ=255) gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg < 2:1446707/120‑1 (MQ=255) cGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa < 1:597712/46‑1 (MQ=255) cGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa > 2:597712/1‑46 (MQ=255) tAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATg > 1:1181773/1‑129 (MQ=255) tAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATg < 2:1181773/129‑1 (MQ=255) aTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGtt < 1:887277/47‑1 (MQ=255) aTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGtt > 2:887277/1‑47 (MQ=255) aTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTa > 1:1531318/1‑48 (MQ=255) aTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTa < 2:1531318/48‑1 (MQ=255) tCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTc > 2:453184/1‑139 (MQ=255) gCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGaa < 2:922010/139‑1 (MQ=38) gCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGaa < 1:217682/139‑1 (MQ=255) tCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCa < 2:1497581/117‑1 (MQ=25) tCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCa > 1:1497581/1‑117 (MQ=25) tGGCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCctct > 2:1343610/1‑139 (MQ=32) | ATTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCT > NC_000913/2068818‑2069075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |