Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,084,773	G→A	G251G (GGC→GGT)	yeeE ←	UPF0394 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,084,773	0	G	A	100.0%	133.4 / NA	43	G251G (GGC→GGT)	yeeE	UPF0394 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/21);  total (22/21)

TGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCG  >  NC_000913/2084637‑2084905                                                                                                                                           |                                                                                                                                     tGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                    <  2:292604/139‑1 (MQ=255) tGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                    <  2:561674/139‑1 (MQ=255)     aCCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                    <  2:1521115/135‑1 (MQ=255)     aCCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                    >  1:1521115/1‑135 (MQ=255)     aCCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAg                                                                                                                                >  1:34081/1‑139 (MQ=255)      ccGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                               <  2:716041/139‑1 (MQ=255)                         caccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCAcc                                                                                                            <  2:216641/139‑1 (MQ=255)                            cTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTAt                                                                                                                       >  1:398438/1‑127 (MQ=255)                            cTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTAt                                                                                                                       <  2:398438/127‑1 (MQ=255)                              aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGa                                                                                                       >  1:531250/1‑139 (MQ=255)                              aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACACACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCAcc                                                                                                            <  1:483404/134‑1 (MQ=255)                              aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACACACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCAcc                                                                                                            >  2:483404/1‑134 (MQ=255)                                     tACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                    >  2:948609/1‑103 (MQ=255)                                     tACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                    <  1:948609/103‑1 (MQ=255)                                     tACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGaaa                                                                                                >  1:998222/1‑139 (MQ=255)                                              ttgtttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAACCACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAat                                                                                       >  2:996279/1‑139 (MQ=255)                                                 tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcccc                                                                                                                                  <  2:951370/93‑1 (MQ=255)                                                 tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcccc                                                                                                                                  >  1:951370/1‑93 (MQ=255)                                                 tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATAtt                                                                                    >  1:748631/1‑139 (MQ=255)                                                 tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATAtt                                                                                    >  1:1341262/1‑139 (MQ=255)                                                   tgtgCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGTAGAATATTgg                                                                                  >  1:990535/1‑139 (MQ=255)                                                       cATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGt                                                                              <  2:1147982/139‑1 (MQ=255)                                                                cgcgAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAACCACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGt                                                                     <  1:996279/139‑1 (MQ=255)                                                                                aCGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAAc                                                     >  1:537553/1‑139 (MQ=255)                                                                                  ggCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTg                                                                                             >  2:1151275/1‑97 (MQ=255)                                                                                  ggCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTg                                                                                             <  1:1151275/97‑1 (MQ=255)                                                                                          cTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGc                                                                                 >  2:14653/1‑103 (MQ=255)                                                                                          cTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGc                                                                                 <  1:14653/103‑1 (MQ=255)                                                                                                  gCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCtt                                     <  2:998222/139‑1 (MQ=255)                                                                                                  gCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCtt                                     <  2:531250/139‑1 (MQ=255)                                                                                                                cACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                           <  1:842777/71‑1 (MQ=255)                                                                                                                cACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                           >  2:842777/1‑71 (MQ=255)                                                                                                                cACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                          <  2:217429/136‑1 (MQ=255)                                                                                                                cACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                          >  1:217429/1‑136 (MQ=255)                                                                                                                  cGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                          >  1:470792/1‑54 (MQ=255)                                                                                                                  cGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                          <  2:470792/54‑1 (MQ=255)                                                                                                                    aaGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTa                   <  2:510201/139‑1 (MQ=255)                                                                                                                          ccTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAgcg             <  2:748631/139‑1 (MQ=255)                                                                                                                             aaCACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCaa          >  2:554922/1‑139 (MQ=255)                                                                                                                               cacCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATc        >  2:1441402/1‑139 (MQ=255)                                                                                                                                 ccAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGc                                       <  1:589422/106‑1 (MQ=255)                                                                                                                                 ccAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGc                                       >  2:589422/1‑106 (MQ=255)                                                                                                                                     gAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCg  >  1:230532/1‑139 (MQ=255)                                                                                                                                           |                                                                                                                                     TGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCT‑‑CGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCG  >  NC_000913/2084637‑2084905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑