Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,111,150	G→A	A276V (GCG→GTG)	rfbD ←	dTDP‑L‑rhamnose synthase, NAD(P)‑dependent dTDP‑4‑dehydrorhamnose reductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,111,150	0	G	A	100.0%	216.6 / NA	67	A276V (GCG→GTG)	rfbD	dTDP‑L‑rhamnose synthase, NAD(P)‑dependent dTDP‑4‑dehydrorhamnose reductase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/34);  total (33/34)

TTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGC  >  NC_000913/2111014‑2111277                                                                                                                                         |                                                                                                                                tttcatttcaTCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGcaca                                                                                                                               >  2:1326214/1‑139 (MQ=255) tttcatttcaTCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGcaca                                                                                                                               <  2:1486502/139‑1 (MQ=255) tttcatttcaTCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGcaca                                                                                                                               >  1:381927/1‑139 (MQ=255)                    ttAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAAttttt                                                                                                            <  2:1572960/139‑1 (MQ=255)                    ttAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAAttttt                                                                                                            >  2:1627/1‑139 (MQ=255)                           aTCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTAtt                                                                                                     >  1:287102/1‑139 (MQ=255)                           aTCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTAtt                                                                                                     <  1:611645/139‑1 (MQ=255)                                gCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGc                                                                                                <  2:697876/139‑1 (MQ=255)                                 cTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCg                                                                                               >  1:731919/1‑139 (MQ=255)                                         tCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAt                                                                                       >  1:445728/1‑139 (MQ=255)                                              aaCAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAAttttt                                                                                                            >  1:150265/1‑113 (MQ=255)                                              aaCAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAAttttt                                                                                                            <  2:150265/113‑1 (MQ=255)                                                 aaGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGaa                                                                                                                  >  1:1287787/1‑104 (MQ=255)                                                 aaGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGaa                                                                                                                  <  2:1287787/104‑1 (MQ=255)                                                 aaGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacga                                                                               <  2:745865/139‑1 (MQ=255)                                                     tGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAg                                                                           <  2:731919/139‑1 (MQ=255)                                                      gCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGc                                                                          <  2:1111006/139‑1 (MQ=255)                                                         aaaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTgg                                                                       <  1:1627/139‑1 (MQ=255)                                                               aTTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAgg                                                                 >  2:98978/1‑139 (MQ=255)                                                                ttAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAg                                                                           <  1:591727/128‑1 (MQ=255)                                                                ttAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAg                                                                           >  2:591727/1‑128 (MQ=255)                                                                ttAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGa                                                                <  2:381927/139‑1 (MQ=255)                                                                ttAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGAAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGa                                                                <  2:287102/139‑1 (MQ=255)                                                                      tGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCt                                                          <  2:267613/139‑1 (MQ=255)                                                                        cTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgt                                                        >  1:608701/1‑139 (MQ=255)                                                                        cTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCt                                                          >  2:1121381/1‑137 (MQ=255)                                                                        cTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCt                                                          <  1:1121381/137‑1 (MQ=255)                                                                             gTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGc                                                                                                <  1:619611/94‑1 (MQ=255)                                                                             gTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGc                                                                                                >  2:619611/1‑94 (MQ=255)                                                                             gTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAgg                                                            >  2:1093853/1‑130 (MQ=255)                                                                             ctCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAgg                                                            <  1:1093853/129‑1 (MQ=255)                                                                                gTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTa                                                <  1:205425/139‑1 (MQ=255)                                                                                  aaaTAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAgg                                                            >  1:1185007/1‑125 (MQ=255)                                                                                  aaaTAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAgg                                                            <  2:1185007/125‑1 (MQ=255)                                                                                   aataatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTg                                             >  2:8708/1‑139 (MQ=255)                                                                                     taatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCg                                           >  2:474927/1‑139 (MQ=255)                                                                                        ttCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACCAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACAAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGtag                                        >  1:292706/1‑137 (MQ=255)                                                                                        ttCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTa                                                <  2:246791/131‑1 (MQ=255)                                                                                        ttCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTa                                                >  1:246791/1‑131 (MQ=255)                                                                                        ttCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTg                                        <  1:15806/139‑1 (MQ=255)                                                                                             tGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCtt                                   >  2:139819/1‑139 (MQ=255)                                                                                             tGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCtt                                   >  1:1262328/1‑139 (MQ=255)                                                                                               aGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCttgt                                 >  2:1611226/1‑139 (MQ=255)                                                                                                gCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCttgtt                                <  2:1260711/139‑1 (MQ=255)                                                                                                 cATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAAttttt                                                                                                            <  2:846428/62‑1 (MQ=255)                                                                                                 cATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAAttttt                                                                                                            >  1:846428/1‑62 (MQ=255)                                                                                                 cATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGgt                                                                      >  2:1126837/1‑100 (MQ=255)                                                                                                 cATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGgt                                                                      <  1:1126837/100‑1 (MQ=255)                                                                                                   ttCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAg                             >  2:445550/1‑139 (MQ=255)                                                                                                    tCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGc                                                                          <  1:1234468/93‑1 (MQ=255)                                                                                                    tCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGc                                                                          >  2:1234468/1‑93 (MQ=255)                                                                                                         tCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacga                                                                               >  1:67897/1‑83 (MQ=255)                                                                                                         tCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacga                                                                               <  2:67897/83‑1 (MQ=255)                                                                                                           aCGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAggg                     <  1:680426/139‑1 (MQ=255)                                                                                                                aaCCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATg                >  1:613275/1‑139 (MQ=255)                                                                                                                  ccTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacga                                                                               <  2:106393/74‑1 (MQ=255)                                                                                                                  ccTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacga                                                                               >  1:106393/1‑74 (MQ=255)                                                                                                                  ccTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAg                             <  1:184353/124‑1 (MQ=255)                                                                                                                  ccTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAg                             >  2:184353/1‑124 (MQ=255)                                                                                                                       cAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGacgacg                                                                             <  2:714062/71‑1 (MQ=255)                                                                                                                       cAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGacgacg                                                                             >  1:714062/1‑71 (MQ=255)                                                                                                                              gCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTAtt                                                                                                     <  1:1225455/40‑1 (MQ=255)                                                                                                                              gCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTAtt                                                                                                     >  2:1225455/1‑40 (MQ=255)                                                                                                                              gCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGa                                                                >  2:1245110/1‑77 (MQ=255)                                                                                                                              gCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGa                                                                <  1:1245110/77‑1 (MQ=255)                                                                                                                              gCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTgcgcgc  <  2:613275/139‑1 (MQ=255)                                                                                                                                 aGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGaa                  <  2:1502722/120‑1 (MQ=255)                                                                                                                                 aGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGaa                  >  1:1502722/1‑120 (MQ=255)                                                                                                                                   aCAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCga                                                                                              >  1:948348/1‑42 (MQ=255)                                                                                                                                   aCAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCga                                                                                              <  2:948348/42‑1 (MQ=255)                                                                                                                                         |                                                                                                                                TTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGC  >  NC_000913/2111014‑2111277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑