Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,115,907 C→T *407* (TAG→TAA wcaL ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,115,9070CT100.0% 92.6 / NA 30*407* (TAG→TAAwcaLputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/14);  total (16/14)

TGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCA  >  NC_000913/2115771‑2116037
                                                                                                                                           |                                                                                                                                  
tgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCTTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTta                                                                                                                                  >  2:1022850/1‑139 (MQ=255)
tgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTta                                                                                                                                  >  1:670619/1‑139 (MQ=255)
              tAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTgcagca                                                                                                                    >  2:269385/1‑139 (MQ=255)
                   ttAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAg                                                                                                                  <  1:270006/139‑1 (MQ=255)
                       cGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTgcagc                                                                                                                     >  2:969645/1‑129 (MQ=255)
                       cGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTgcagc                                                                                                                     <  1:969645/129‑1 (MQ=255)
                        gCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAg                                                                                                          <  1:1022850/139‑1 (MQ=255)
                           ttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGc                                                                                                                            <  1:1040626/118‑1 (MQ=255)
                           ttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGc                                                                                                                            >  2:1040626/1‑118 (MQ=255)
                           ttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                             >  1:534484/1‑133 (MQ=255)
                           ttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                             <  2:534484/133‑1 (MQ=255)
                            tGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtct                                                                                                      >  1:426731/1‑139 (MQ=255)
                                                           tAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGtt                                                                       >  2:85915/1‑139 (MQ=255)
                                                                        ggCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGa                                                                                                   <  1:1583019/101‑1 (MQ=255)
                                                                        ggCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGa                                                                                                   >  2:1583019/1‑101 (MQ=255)
                                                                        ggCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTc                                                             >  2:215215/1‑139 (MQ=255)
                                                                             gcgcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCt                                                                                                                          <  2:1563837/73‑1 (MQ=255)
                                                                             gcgcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCt                                                                                                                          >  1:1563837/1‑73 (MQ=255)
                                                                             gcgcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcg                                                        <  1:269385/139‑1 (MQ=255)
                                                                                              aGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                        <  1:374618/90‑1 (MQ=255)
                                                                                              aGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                        >  2:374618/1‑90 (MQ=255)
                                                                                                       cGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATtcg                              >  2:1574194/1‑139 (MQ=255)
                                                                                                        gTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATtcgt                             <  1:85915/139‑1 (MQ=255)
                                                                                                                     tttAAATGGCATGCGTTCTCCTTTATAAAGCCTgcag                                                                                                                      >  2:715008/1‑37 (MQ=255)
                                                                                                                     tttAAATGGCATGCGTTCTCCTTTATAAAGCCTgcag                                                                                                                      <  1:715008/37‑1 (MQ=255)
                                                                                                                      ttAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAt                                                                                                  <  1:1197408/56‑1 (MQ=255)
                                                                                                                      ttAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAt                                                                                                  >  2:1197408/1‑56 (MQ=255)
                                                                                                                            ggCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGt                  <  2:628575/130‑1 (MQ=255)
                                                                                                                            ggCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGt                  >  1:628575/1‑130 (MQ=255)
                                                                                                                                  cGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACgccgcc   <  1:1574194/139‑1 (MQ=255)
                                                                                                                                   gTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCATCTTTTTCGCGCGTGCGTTTGACCACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCa  <  1:504643/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                  
TGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAT‑‑‑GGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCA  >  NC_000913/2115771‑2116037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: