Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 200,214 C→T P763S (CCA→TCA)  bamA → BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913200,2140CT98.8% 271.4 / NA 85P763S (CCA→TCA) bamABamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (42/42);  minor base G (1/0);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCGGT  >  NC_000913/200084‑200348
                                                                                                                                  |                                                                                                                                      
caCCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAat                                                                                                                                <  1:757114/139‑1 (MQ=255)
caCCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAat                                                                                                                                <  2:290454/139‑1 (MQ=255)
    ccGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATcc                                                                                                                            >  1:1406915/1‑139 (MQ=255)
     cGACGCCGTTTATTAGCGATAAGTTTGCTAACTCGGTTCGTACTTGCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCTAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCg                                                                                                                           >  2:993649/1‑139 (MQ=255)
     cGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTCCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCg                                                                                                                           >  1:1144164/1‑139 (MQ=255)
                 ttaGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                               <  2:799230/139‑1 (MQ=255)
                   aGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGTTa                                                                                                             >  1:387284/1‑139 (MQ=255)
                     cGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATc                                                                                                           >  2:929577/1‑139 (MQ=255)
                       aTAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGc                                                                                                         <  2:1226253/139‑1 (MQ=255)
                        tAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa                                                                                                        <  2:1144164/139‑1 (MQ=255)
                         aaGTATGCTAACTCGGTTCGTACTTCCTACTTCTGTGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCAGTATGTCTGCGGGTATCGCAt                                                                                                       <  1:1493457/139‑1 (MQ=255)
                              tGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACaa                                                                                                  >  2:1101164/1‑139 (MQ=255)
                                cTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTa                                                                                                                         >  2:554762/1‑114 (MQ=255)
                                cTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTa                                                                                                                         <  1:554762/114‑1 (MQ=255)
                                   aCTCGGTTCATACTTGTTTCTTCGGCGATATTGGTACCGTTTGGGATACAAACTGGGATAGCAGCCCATACTCTGGATATCCGGACTATAGTGAGTCAAGCAATATCCGTATGTCTGCGGATATCGCATTACAATGGAt                                                                                             <  1:272287/139‑1 (MQ=255)
                                    cTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAg                                                                                                                                    >  1:1411108/1‑99 (MQ=255)
                                    cTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAg                                                                                                                                    <  2:1411108/99‑1 (MQ=255)
                                    cTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTa                                                                                                                         >  2:526594/1‑110 (MQ=255)
                                    cTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTa                                                                                                                         <  1:526594/110‑1 (MQ=255)
                                    cTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                               <  2:1203950/120‑1 (MQ=255)
                                    cTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                               >  1:1203950/1‑120 (MQ=255)
                                    cTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATg                                                                                            >  1:972271/1‑139 (MQ=255)
                                        gTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTcc                                                                                         <  1:1598922/138‑1 (MQ=255)
                                        gTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTcc                                                                                         >  2:1598922/1‑138 (MQ=255)
                                         ttCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAg                                                                                                                                    >  1:274572/1‑94 (MQ=255)
                                         ttCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAg                                                                                                                                    <  2:274572/94‑1 (MQ=255)
                                                    ttcttcTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGt                                                                            <  2:1312198/139‑1 (MQ=255)
                                                     tcttctGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGtt                                                                           >  2:1410481/1‑139 (MQ=255)
                                                      cttctGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTg                                                                          <  1:720105/139‑1 (MQ=255)
                                                      cttctGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTg                                                                          <  2:387284/139‑1 (MQ=255)
                                                          tGGGATATGGGTACCGTTTGGGATACAAACTGGGATACCAGCCAATACTCTGGATATCCGGACAATAGTGATTCAAGCAATATCCGTCTGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgtgt                                                                      >  2:1027783/1‑139 (MQ=255)
                                                           gggATATGGGTACCGTTTGGGATACAAACTGGTATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGtt                                                                     <  1:142292/139‑1 (MQ=255)
                                                            ggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACa                                                                                                   >  2:1028333/1‑108 (MQ=255)
                                                            ggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACa                                                                                                   <  1:1028333/108‑1 (MQ=255)
                                                            ggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGAt                                                                                             >  2:410672/1‑114 (MQ=255)
                                                            ggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGAt                                                                                             <  1:410672/114‑1 (MQ=255)
                                                            ggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTtc                                                                    >  1:1211726/1‑139 (MQ=255)
                                                            ggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTtc                                                                    >  2:1244920/1‑139 (MQ=255)
                                                                atGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCt                                                                >  1:399940/1‑139 (MQ=255)
                                                                 tGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTa                                                               >  1:790959/1‑139 (MQ=255)
                                                                   ggTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa                                                                                                                                  >  1:1256641/1‑70 (MQ=255)
                                                                   ggTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa                                                                                                                                  <  2:1256641/70‑1 (MQ=255)
                                                                   ggTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATc                                                                                                           <  1:1103528/93‑1 (MQ=255)
                                                                   ggTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATc                                                                                                           >  2:1103528/1‑93 (MQ=255)
                                                                   ggTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGtt                                                                     >  2:501238/1‑131 (MQ=255)
                                                                   ggTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGtt                                                                     <  1:501238/131‑1 (MQ=255)
                                                                       ccGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAatat                                                                                                                              >  2:1407702/1‑70 (MQ=255)
                                                                       ccGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAatat                                                                                                                              <  1:1407702/70‑1 (MQ=255)
                                                                            tGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAat                                                                                                                                >  2:1124136/1‑63 (MQ=255)
                                                                            tGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAat                                                                                                                                <  1:1124136/63‑1 (MQ=255)
                                                                            tGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTa                                                                                                             >  1:74491/1‑82 (MQ=255)
                                                                            tGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTa                                                                                                             <  2:74491/82‑1 (MQ=255)
                                                                                    aaaCTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                               >  2:1130306/1‑72 (MQ=255)
                                                                                    aaaCTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                               <  1:1130306/72‑1 (MQ=255)
                                                                                    aaaCTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCACCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAg                                            >  2:1584888/1‑139 (MQ=255)
                                                                                        tGGGATTCCAGCCAATATTCTGGATATTCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCAGTGGGGCCGTTGGTGTACTCCTACGCCCAGCCGTTCAAAAAGTACg                                        <  1:929577/139‑1 (MQ=255)
                                                                                        tGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACg                                        <  2:1406915/139‑1 (MQ=255)
                                                                                         gggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGa                                       >  2:548009/1‑139 (MQ=255)
                                                                                          ggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGAt                                      >  1:704794/1‑139 (MQ=255)
                                                                                          ggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGAt                                      >  2:1459720/1‑139 (MQ=255)
                                                                                               ccAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGgaga                                 <  1:1584888/139‑1 (MQ=255)
                                                                                               ccAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGgaga                                 <  2:1211726/139‑1 (MQ=255)
                                                                                               ccAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGgaga                                 <  2:399940/139‑1 (MQ=255)
                                                                                               ccAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGgaga                                 <  2:394762/139‑1 (MQ=255)
                                                                                                  gCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAatat                                                                                                                              <  1:1603582/43‑1 (MQ=255)
                                                                                                  gCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAatat                                                                                                                              >  2:1603582/1‑43 (MQ=255)
                                                                                                             ggATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                               >  2:644205/1‑47 (MQ=255)
                                                                                                             ggATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                               <  1:644205/47‑1 (MQ=255)
                                                                                                                 atCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCg                                                                                                          >  1:1296686/1‑48 (MQ=255)
                                                                                                                 atCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCg                                                                                                          <  2:1296686/48‑1 (MQ=255)
                                                                                                                 atCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGt                                           >  1:604041/1‑111 (MQ=255)
                                                                                                                 atCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGt                                           <  2:604041/111‑1 (MQ=255)
                                                                                                                 atCCGGACTATAGTGATGCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCa               >  2:1007978/1‑139 (MQ=255)
                                                                                                                  tCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGtt                                                                     >  2:1068971/1‑84 (MQ=255)
                                                                                                                  tCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGtt                                                                     <  1:1068971/84‑1 (MQ=255)
                                                                                                                   ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGt             >  2:32889/1‑139 (MQ=255)
                                                                                                                   ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGt             >  2:727647/1‑139 (MQ=255)
                                                                                                                        cTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTaat        <  1:1007978/139‑2 (MQ=255)
                                                                                                                           taGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCAt                                                                                                       <  1:608473/41‑1 (MQ=255)
                                                                                                                           taGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCAt                                                                                                       >  2:608473/1‑41 (MQ=255)
                                                                                                                            aGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACACTGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCt                                                                >  1:1143836/1‑79 (MQ=255)
                                                                                                                            aGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGt                                                                            >  1:1202202/1‑67 (MQ=255)
                                                                                                                            aGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGt                                                                            <  2:1202202/67‑1 (MQ=255)
                                                                                                                            aGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCt                                                                <  2:1143836/79‑1 (MQ=255)
                                                                                                                              tGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCGGt  <  1:1410481/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                      
CACCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCGGT  >  NC_000913/200084‑200348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: