Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,195,459	T→C	V387A (GTT→GCT)	metG →	methionyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,195,459	0	T	C	100.0%	184.0 / NA	51	V387A (GTT→GCT)	metG	methionyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/26);  total (25/26)

AAGCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTGA  >  NC_000913/2195327‑2195591                                                                                                                                     |                                                                                                                                     aaGCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACaa                                                                                                                                <  1:662573/139‑1 (MQ=255)   gCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAg                                                                                                                              >  2:223492/1‑139 (MQ=255)              gCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTg                                                                                                                   <  2:1301711/139‑1 (MQ=255)                      aTTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCg                                                                                                           <  1:223492/139‑1 (MQ=255)                             cGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCg                                                                                                    <  1:1610364/139‑1 (MQ=255)                                    aGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATg                                                                                                      >  2:527509/1‑130 (MQ=255)                                    aGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATg                                                                                                      <  1:527509/130‑1 (MQ=255)                                      ccTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATc                                                                                           >  2:137762/1‑139 (MQ=255)                                                    acacTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCg                                                                                                           <  1:1243174/109‑1 (MQ=255)                                                    acacTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCg                                                                                                           >  2:1243174/1‑109 (MQ=255)                                                       cTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACgg                                                                          >  1:31156/1‑139 (MQ=255)                                                        tGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCACTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTa                                                                                             <  1:1453319/119‑1 (MQ=255)                                                        tGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCACTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTa                                                                                             >  2:1453319/1‑119 (MQ=255)                                                         gCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCg                                                                        >  2:1107087/1‑139 (MQ=255)                                                          cGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGt                                                                       >  1:449648/1‑139 (MQ=255)                                                          cGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGt                                                                       >  1:1155216/1‑139 (MQ=255)                                                           gAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTg                                                                      <  1:137762/139‑1 (MQ=255)                                                                  tctTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCaa                                                               >  2:113148/1‑139 (MQ=255)                                                                      cgcgCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGa                                                           <  2:568101/139‑1 (MQ=255)                                                                                   tatCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCg                                              <  2:449648/139‑1 (MQ=255)                                                                                   tatCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCg                                              <  2:1155216/139‑1 (MQ=255)                                                                                    atCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGc                                             <  1:266943/139‑1 (MQ=255)                                                                                        aTCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACaaa                                                                                                                               <  1:390200/53‑1 (MQ=255)                                                                                        aTCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACaaa                                                                                                                               >  2:390200/1‑53 (MQ=255)                                                                                        aTCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTg                                                                                                                   <  1:962922/65‑1 (MQ=255)                                                                                        aTCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTg                                                                                                                   >  2:962922/1‑65 (MQ=255)                                                                                         tctcAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAGCAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATcaaca                                                                                       <  1:1141398/92‑1 (MQ=255)                                                                                         tctcAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 <  2:938736/66‑1 (MQ=255)                                                                                         tctcAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 >  1:938736/1‑66 (MQ=255)                                                                                         tctcAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATcaaca                                                                                       >  2:1141398/1‑92 (MQ=255)                                                                                             aaCCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGtt                                                                                 <  1:174972/94‑1 (MQ=255)                                                                                             aaCCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGtt                                                                                 >  2:174972/1‑94 (MQ=255)                                                                                             aaCCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGt                                          >  1:1071187/1‑133 (MQ=255)                                                                                             aaCCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGt                                          <  2:1071187/133‑1 (MQ=255)                                                                                             aaCCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACa                                    >  1:184843/1‑139 (MQ=255)                                                                                              aCCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACaa                                   <  2:184843/139‑1 (MQ=255)                                                                                              aCCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACaa                                   >  1:927702/1‑139 (MQ=255)                                                                                                   gAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCt                              >  1:42144/1‑139 (MQ=255)                                                                                                   gAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCt                              >  1:1201178/1‑139 (MQ=255)                                                                                                    aaGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAAc                                                                                                                      <  1:727639/50‑1 (MQ=255)                                                                                                    aaGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAAc                                                                                                                      >  2:727639/1‑50 (MQ=255)                                                                                                      gATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCa                           <  2:42144/139‑1 (MQ=255)                                                                                                       aTTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCg                                              >  2:1541041/1‑119 (MQ=255)                                                                                                       aTTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCg                                              <  1:1541041/119‑1 (MQ=255)                                                                                                       aTTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCAc                          >  2:239387/1‑139 (MQ=255)                                                                                                         ttCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTg                        >  1:578971/1‑139 (MQ=255)                                                                                                               cAGCGTGTGCATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCg                  <  1:1107087/139‑1 (MQ=255)                                                                                                                  cGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCg                                              <  2:679251/108‑1 (MQ=255)                                                                                                                  cGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCg                                              >  1:679251/1‑108 (MQ=255)                                                                                                                             cGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGt    <  2:1201178/139‑1 (MQ=255)                                                                                                                               atatCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTGa  <  2:1150612/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     AAGCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTGA  >  NC_000913/2195327‑2195591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑