Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,220,195	A→G	F599L (TTC→CTC)	bglX ←	beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,220,195	0	A	G	100.0%	197.0 / NA	55	F599L (TTC→CTC)	bglX	beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/22);  total (33/22)

AACGGATACAACGCCCCGTTAGCTTCATCAAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAAGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCATCAGCCTGCTGATCTTCTTTCACCAGCGCCA  >  NC_000913/2220065‑2220326                                                                                                                                   |                                                                                                                                    aaCGGATACAACGCCCCGTTAGCTTCATCAAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTg                                                                                                                             >  2:864334/1‑139 (MQ=255) aaCGGATACAACGCCCCGTTAGCTTCATCAAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTg                                                                                                                             >  2:317496/1‑139 (MQ=255) aaCGGATACAACGCCCCGTTAGCTTCATCAAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTg                                                                                                                             >  2:1501999/1‑139 (MQ=255) aaCGGATACAACGCCCCGTTAGCTTCATCAAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTg                                                                                                                             >  1:171131/1‑139 (MQ=255)        aCAACGCCCCGTTAGCTTCATCAAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGAGATTGGCAGCtt                                                                                                                      >  2:1048490/1‑139 (MQ=255)          aaCGCCCCGTTAGCTTCATCAAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGc                                                                                                                    <  1:864334/139‑1 (MQ=255)           aCGCCCCGTTAGCTTCATCAAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGcc                                                                                                                   >  1:1284987/1‑139 (MQ=255)                      gCTTCATCAAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGt                                                                                                        <  1:1048490/139‑1 (MQ=255)                           atcaAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCg                                                                                                   >  2:676525/1‑139 (MQ=255)                           atcaAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCg                                                                                                   <  1:143849/139‑1 (MQ=255)                           atcaAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCg                                                                                                   <  1:317496/139‑1 (MQ=255)                            tcaAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGc                                                                                                  >  2:800512/1‑139 (MQ=255)                             caAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGcc                                                                                                 <  2:1329980/139‑1 (MQ=255)                              aaaaTAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCa                                                                                                <  2:99559/139‑1 (MQ=255)                                    aCGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAAt                                                                                          <  1:1501999/139‑1 (MQ=255)                                          aGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGcc                                                                                                 <  1:500938/126‑1 (MQ=255)                                          aGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGcc                                                                                                 >  2:500938/1‑126 (MQ=255)                                                    ttCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGcc                                                                                                 >  1:1097174/1‑116 (MQ=255)                                                    ttCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGcc                                                                                                 <  2:1097174/116‑1 (MQ=255)                                                      cGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTa                                                                        >  1:341994/1‑139 (MQ=255)                                                      cGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTa                                                                        >  1:605758/1‑139 (MQ=255)                                                        gCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAACCAATACATCGGCAATTGCATTAcc                                                                      <  1:1552406/139‑1 (MQ=255)                                                        gCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGc                                                                                  >  2:721875/1‑127 (MQ=255)                                                        gCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGc                                                                                  <  1:721875/127‑1 (MQ=255)                                                        gCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTAcc                                                                      >  1:884877/1‑139 (MQ=255)                                                            gTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCt                                                                  >  2:34789/1‑139 (MQ=255)                                                             tCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACAt                                                                                      <  2:1136082/118‑1 (MQ=255)                                                             tCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACAt                                                                                      >  1:1136082/1‑118 (MQ=255)                                                                 cATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGt                                                             <  1:676525/139‑1 (MQ=255)                                                                       aCGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCAtt                                                                         <  2:683310/121‑1 (MQ=255)                                                                       aCGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCAtt                                                                         >  1:683310/1‑121 (MQ=255)                                                                           gCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGc                                                                                                  >  1:1520008/1‑92 (MQ=255)                                                                           gCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGc                                                                                                  <  2:1520008/92‑1 (MQ=255)                                                                           gCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAAc                                                   >  1:470983/1‑139 (MQ=255)                                                                           gCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAAc                                                   >  1:742030/1‑139 (MQ=255)                                                                           gCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAAc                                                   >  1:652046/1‑139 (MQ=255)                                                                                             gCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGTAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGCATCGCa                                 >  1:1259421/1‑139 (MQ=255)                                                                                             gCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCa                                 >  1:971089/1‑139 (MQ=255)                                                                                             gCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCa                                 >  1:503908/1‑139 (MQ=255)                                                                                             gCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCa                                 <  2:652046/139‑1 (MQ=255)                                                                                             gCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCa                                 >  2:437980/1‑139 (MQ=255)                                                                                             gCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCa                                 <  1:800512/139‑1 (MQ=255)                                                                                                gtagtaCACCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCATCa                              <  2:605758/139‑1 (MQ=255)                                                                                                       acCGGGATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCATCAGCctgct                       >  2:1019450/1‑139 (MQ=255)                                                                                                          gggATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCATCAGCCTGCTGAt                    <  2:971089/139‑1 (MQ=255)                                                                                                           ggATCTGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCATCAGCCTGCTGAtc                   >  2:1387586/1‑139 (MQ=255)                                                                                                                tGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGc                                                                                                  <  1:1325702/55‑1 (MQ=255)                                                                                                                tGCCCGACAGAACGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGc                                                                                                  >  2:1325702/1‑55 (MQ=255)                                                                                                                          aaCGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCATCAGCCTGCTGATCTTCTTTCAccagcgc    <  2:171131/139‑1 (MQ=255)                                                                                                                          aaCGCGGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCATCAGCCTGCTGATCTTCTTTCAccagcgc    >  1:476825/1‑139 (MQ=255)                                                                                                                            cgcgGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCAt                                >  2:1067794/1‑109 (MQ=255)                                                                                                                            cgcgGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCAt                                <  1:1067794/109‑1 (MQ=255)                                                                                                                            cgcgGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCATCAGCCTGCTGATCTTCTTTCAccagcgcca  >  1:1554074/1‑139 (MQ=255)                                                                                                                            cgcgGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCATCAGCCTGCTGATCTTCTTTCAccagcgcca  >  2:685636/1‑139 (MQ=255)                                                                                                                            cgcgGGAGGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCATCAGCCTGCTGATCTTCTTTCAccagcgcca  >  1:1079634/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                    AACGGATACAACGCCCCGTTAGCTTCATCAAAATAACGCGAAGTGTATTTGTTCGGCTTGTCGGCATTATACGGGCGACCGGTATTCAGATGGCTGTAGTACACCGGGATCTGCCCGACAGAACGCGGGAAGGACATTGGCAGCTTGCCGGACGGGTTGTAATCGCCAAACAATACATCGGCAATTGCATTACCGCCTTCAGTCCCCGCAAACCAGGTTTCCAGAATCGCATCAGCCTGCTGATCTTCTTTCACCAGCGCCA  >  NC_000913/2220065‑2220326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑