Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,276,298	G→A	*342* (TAG→TAA)	yejE →	microcin C ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,276,298	0	G	A	100.0%	226.6 / NA	70	*342* (TAG→TAA)	yejE	microcin C ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/36);  total (34/36)

AGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAG  >  NC_000913/2276169‑2276432                                                                                                                                  |                                                                                                                                       aGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCa                                                                                                                               <  1:454011/139‑1 (MQ=255) aGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCa                                                                                                                               >  2:78580/1‑139 (MQ=255)   ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAGGGCGGTGTAACATGACGCaaa                                                                                                                             >  2:948119/1‑139 (MQ=255)   ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCaaa                                                                                                                             <  2:1189840/139‑1 (MQ=255)   ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCaaa                                                                                                                             >  1:1457715/1‑139 (MQ=255)             ccTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGc                                                                                                                   >  1:897993/1‑139 (MQ=255)              cTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCa                                                                                                                               <  2:1551978/126‑1 (MQ=255)              cTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCa                                                                                                                               >  1:1551978/1‑126 (MQ=255)                   ggCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGa                                                                                                             <  1:1526480/139‑1 (MQ=255)                    gCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGaa                                                                                                            >  1:1008644/1‑139 (MQ=255)                              ttGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAg                                                                                                                    >  2:1570821/1‑121 (MQ=255)                              ttGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAg                                                                                                                    <  1:1570821/121‑1 (MQ=255)                                gggATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTg                                                                                                >  1:1510111/1‑139 (MQ=255)                                  gATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTggg                                                                                              <  1:835736/139‑1 (MQ=255)                                                  tCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                              <  2:1457715/139‑1 (MQ=255)                                                    ggTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc                                                                            <  2:1510111/139‑1 (MQ=255)                                                       ggCGGTATTATTGTCATAGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAGGGCGGTGTAACATGACGAAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                         <  1:78580/139‑1 (MQ=255)                                                              ttattGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa                                                                  <  2:897993/139‑1 (MQ=255)                                                                      tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAAt                                                                                                         <  2:1590827/92‑1 (MQ=255)                                                                      tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAAt                                                                                                         >  1:1590827/1‑92 (MQ=255)                                                                      tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGGAAGTACag                                                                 >  1:748115/1‑132 (MQ=255)                                                                      tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACAGTACGTACAGTAGTCaa                                                          <  2:1008644/139‑1 (MQ=255)                                                                      tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACGCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACag                                                                 <  2:748115/132‑1 (MQ=255)                                                                        tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGc                                                                                >  2:843558/1‑115 (MQ=255)                                                                        tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGc                                                                                <  1:843558/115‑1 (MQ=255)                                                                        tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatg                                                        >  2:142346/1‑139 (MQ=255)                                                                        tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatg                                                        >  2:1460949/1‑139 (MQ=255)                                                                        tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatg                                                        >  1:1599486/1‑139 (MQ=255)                                                                         gCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       <  1:142346/139‑1 (MQ=255)                                                                         gCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       <  2:1418688/139‑1 (MQ=255)                                                                         gCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       <  2:1187654/139‑1 (MQ=255)                                                                             aTCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGtt                                                   <  1:1460949/139‑1 (MQ=255)                                                                                  tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCaaa                                                                                                                             <  1:1453366/60‑1 (MQ=255)                                                                                  tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCaaa                                                                                                                             >  2:1453366/1‑60 (MQ=255)                                                                                  tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                         <  2:1107601/112‑1 (MQ=255)                                                                                  tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                         >  1:1107601/1‑112 (MQ=255)                                                                                  tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACt                                              >  2:848823/1‑139 (MQ=255)                                                                                  tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACt                                              <  2:215262/139‑1 (MQ=255)                                                                                   aTTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTa                                             <  2:1599486/139‑1 (MQ=255)                                                                                       gTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGc                                                                                       >  2:599997/1‑93 (MQ=255)                                                                                       gTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGc                                                                                       <  1:599997/93‑1 (MQ=255)                                                                                         gAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCa                                                                                                                               >  1:1327933/1‑51 (MQ=255)                                                                                         gAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCa                                                                                                                               <  2:1327933/51‑1 (MQ=255)                                                                                         gAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACag                                                                 <  2:1064507/113‑1 (MQ=255)                                                                                         gAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACag                                                                 >  1:1064507/1‑113 (MQ=255)                                                                                         gAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGAtt                                       >  2:1130007/1‑139 (MQ=255)                                                                                          aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAAct                                                                                                                           >  2:1436927/1‑54 (MQ=255)                                                                                          aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAAct                                                                                                                           <  1:1436927/54‑1 (MQ=255)                                                                                          aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGcc                                                                                      <  2:324028/91‑1 (MQ=255)                                                                                          aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGcc                                                                                      >  1:324028/1‑91 (MQ=255)                                                                                          aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       <  2:919761/122‑1 (MQ=255)                                                                                          aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       >  1:919761/1‑122 (MQ=255)                                                                                            gCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAcc                                                                           <  2:1347877/100‑1 (MQ=255)                                                                                            gCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAcc                                                                           >  1:1347877/1‑100 (MQ=255)                                                                                                 ccGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       >  1:812756/1‑115 (MQ=255)                                                                                                 ccGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       <  2:812756/115‑1 (MQ=255)                                                                                                          aTTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgct                      >  1:881412/1‑139 (MQ=255)                                                                                                                    aataaGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa                                                                  >  2:1377606/1‑85 (MQ=255)                                                                                                                    aataaGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa                                                                  <  1:1377606/85‑1 (MQ=255)                                                                                                                      taaGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtggg          <  2:881412/139‑1 (MQ=255)                                                                                                                      taaGGCGGTGCAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGc                                  >  2:114489/1‑115 (MQ=255)                                                                                                                      taaGGCGGTGCAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGc                                  <  1:114489/115‑1 (MQ=255)                                                                                                                          gCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta                                                                    >  2:221210/1‑77 (MQ=255)                                                                                                                          gCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta                                                                    <  1:221210/77‑1 (MQ=255)                                                                                                                            ggTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                              <  2:654867/65‑1 (MQ=255)                                                                                                                            ggTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                              >  1:654867/1‑65 (MQ=255)                                                                                                                              tgtAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGt                                                    >  2:377417/1‑89 (MQ=255)                                                                                                                              tgtAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGt                                                    <  1:377417/89‑1 (MQ=255)                                                                                                                              tgtAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAg  >  1:1343898/1‑139 (MQ=255)                                                                                                                               gtAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatgatg                                                     >  1:144086/1‑87 (MQ=255)                                                                                                                                  |                                                                                                                                       AGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAG  >  NC_000913/2276169‑2276432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑