Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	28,269	C→T	intergenic (+62/‑105)	rihC → / → dapB	ribonucleoside hydrolase 3/dihydrodipicolinate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	28,269	0	C	T	100.0%	152.0 / NA	48	intergenic (+62/‑105)	rihC/dapB	ribonucleoside hydrolase 3/dihydrodipicolinate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/22);  total (26/22)

TGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCCCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATCC  >  NC_000913/28140‑28392                                                                                                                                  |                                                                                                                            tGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATc                                                                                                                    >  1:782237/1‑139 (MQ=255)   gCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTAATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCAt                                                                                                                  >  1:985828/1‑139 (MQ=255)     aTTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTa                                                                                                                >  1:1099233/1‑139 (MQ=255)     aTTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTa                                                                                                                >  1:416737/1‑139 (MQ=255)       tGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAAt                                                                                                              >  1:100460/1‑139 (MQ=255)                                 cagTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggctgg                                                                                    <  2:1596257/139‑1 (MQ=255)                                 cagTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggctgg                                                                                    <  1:69197/139‑1 (MQ=255)                                           cTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCg                                                                          <  2:782237/139‑1 (MQ=255)                                               ggTGCTGGCTCTGGTGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATgc                                                                      <  2:804335/139‑1 (MQ=255)                                                gTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATgcg                                                                     >  1:831097/1‑139 (MQ=255)                                                 tGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATgcgc                                                                    >  2:392462/1‑139 (MQ=255)                                                    tGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGAC‑CATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGt                                                                <  1:726669/139‑1 (MQ=255)                                                        tctGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTTTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTAc                                                             <  1:1187554/139‑1 (MQ=255)                                                                    aCCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGgcggcg                                                                               >  1:1308975/1‑109 (MQ=255)                                                                    aCCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGgcggcg                                                                               <  2:1308975/109‑1 (MQ=255)                                                                         tCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCG‑TTTTGGCTGGCGGCGTAGCg                                                                          <  2:269367/108‑1 (MQ=255)                                                                         tCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCG‑TTTTGGCTGGCGGCGTAGCg                                                                          >  1:269367/1‑108 (MQ=255)                                                                            caTGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCaa                                         <  2:1099233/139‑1 (MQ=255)                                                                              tGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGgcggcg                                                                               >  2:1395933/1‑99 (MQ=255)                                                                              tGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGgcggcg                                                                               <  1:1395933/99‑1 (MQ=255)                                                                                     ggCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCa                                                                                                  >  2:1461534/1‑73 (MQ=255)                                                                                     ggCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCa                                                                                                  <  1:1461534/73‑1 (MQ=255)                                                                                         tGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAagag                            >  1:309848/1‑139 (MQ=255)                                                                                           cAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGt                                                                >  1:37303/1‑101 (MQ=255)                                                                                           cAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGt                                                                <  1:364569/101‑1 (MQ=255)                                                                                           cAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGt                                                                >  2:364569/1‑101 (MQ=255)                                                                                           cAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGt                                                                <  2:37303/101‑1 (MQ=255)                                                                                           cAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGaa                          >  1:668804/1‑139 (MQ=255)                                                                                               cattcatCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGc                      <  2:416737/139‑1 (MQ=255)                                                                                                   catCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATg                  >  1:888554/1‑139 (MQ=255)                                                                                                   catCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATg                  >  2:927816/1‑139 (MQ=255)                                                                                                     tCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCg                                                                          <  2:1503352/81‑1 (MQ=255)                                                                                                     tCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCg                                                                          >  1:1503352/1‑81 (MQ=255)                                                                                                      cGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGAt                                                                        >  2:777020/1‑82 (MQ=255)                                                                                                      cGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGAt                                                                        <  1:777020/82‑1 (MQ=255)                                                                                                          tcctGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGt                                                                <  2:855514/83‑1 (MQ=255)                                                                                                          tCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGt                                                                >  1:855514/1‑86 (MQ=255)                                                                                                            aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggctgg                                                                                    >  2:789948/1‑64 (MQ=255)                                                                                                            aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggctgg                                                                                    <  1:789948/64‑1 (MQ=255)                                                                                                            aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggct                                                                                      >  1:938583/1‑62 (MQ=255)                                                                                                            aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggct                                                                                      <  2:938583/62‑1 (MQ=255)                                                                                                            aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACaaaa                               >  2:824024/1‑117 (MQ=255)                                                                                                            aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACaaaa                               <  1:824024/117‑1 (MQ=255)                                                                                                            aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCa         >  1:1032407/1‑139 (MQ=255)                                                                                                                cTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACa     <  1:927816/139‑1 (MQ=255)                                                                                                                   tCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATcc  >  1:971013/1‑139 (MQ=255)                                                                                                                         atatCCCTCTCTGTTTATGATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGaaa                                                      >  1:895583/1‑81 (MQ=255)                                                                                                                         atatCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGaaa                                                      <  2:895583/81‑1 (MQ=255)                                                                                                                            tccctctcTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGtt                                                               <  2:1007913/69‑1 (MQ=255)                                                                                                                            tccctctcTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGtt                                                               >  1:1007913/1‑69 (MQ=255)                                                                                                                            tccctctcTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGaa                          <  2:836898/106‑1 (MQ=255)                                                                                                                            tccctctcTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGaa                          >  1:836898/1‑106 (MQ=255)                                                                                                                                  |                                                                                                                            TGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCCCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATCC  >  NC_000913/28140‑28392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑