Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,365,284	G→A	Q113* (CAG→TAG)	ais ←	putative LPS core heptose(II)‑phosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,365,284	0	G	A	100.0%	213.7 / NA	65	Q113* (CAG→TAG)	ais	putative LPS core heptose(II)‑phosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/30);  total (35/30)

TGAAAATAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTT  >  NC_000913/2365152‑2365417                                                                                                                                     |                                                                                                                                      tGAAAATAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGt                                                                                                                                 >  2:905388/1‑139 (MQ=255)   aaaaTAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCTACTAAATGATcc                                                                                                                               >  2:445910/1‑139 (MQ=255)   aaaaTAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTcc                                                                                                                               >  1:395262/1‑139 (MQ=255)     aaTAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCgg                                                                                                                             <  1:874797/139‑1 (MQ=255)      aTAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGa                                                                                                                            <  1:905388/139‑1 (MQ=255)            atatTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTAt                                                                                                                      <  2:293903/139‑1 (MQ=255)                     tCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAAt                                                                                                             <  1:1110622/139‑1 (MQ=255)                       aGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATaa                                                                                                           <  2:395262/139‑1 (MQ=255)                         gcgcTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCg                                                                                                                              <  2:171086/118‑1 (MQ=255)                         gcgcTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCg                                                                                                                              >  1:171086/1‑118 (MQ=255)                                           ccTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTa                                                                                                                    <  1:1262066/110‑1 (MQ=255)                                           ccTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTa                                                                                                                    >  2:1262066/1‑110 (MQ=255)                                                   gCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTaa                                                                               <  2:1035454/139‑1 (MQ=255)                                                        ataAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCg                                                                          <  2:635269/139‑1 (MQ=255)                                                         taAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTa                                                                                                                    >  1:1104919/1‑96 (MQ=255)                                                         taAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTa                                                                                                                    <  2:1104919/96‑1 (MQ=255)                                                              ctcATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc                                                                                       <  1:1199068/120‑1 (MQ=255)                                                              ctcATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc                                                                                       >  2:1199068/1‑120 (MQ=255)                                                                   tACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGAc                                                                                                                           >  2:672824/1‑79 (MQ=255)                                                                   tACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGAc                                                                                                                           <  1:672824/79‑1 (MQ=255)                                                                   tACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTc                                                               >  1:310618/1‑139 (MQ=255)                                                                        cACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACggg                                                          >  2:1376333/1‑139 (MQ=255)                                                                        cACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACggg                                                          >  1:269501/1‑139 (MQ=255)                                                                           tGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCAt                                                       <  2:310618/139‑1 (MQ=255)                                                                            gaagaaGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATc                                                      <  1:1341485/139‑1 (MQ=255)                                                                             aagaagTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGAc                                                                                                                           <  1:663901/69‑1 (MQ=255)                                                                             aagaagTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGAc                                                                                                                           >  2:663901/1‑69 (MQ=255)                                                                             aagaagTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATcc                                                     >  2:691160/1‑139 (MQ=255)                                                                                 agTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTggg                                                 <  1:1376333/139‑1 (MQ=255)                                                                                   tcatttATCTAGCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAACTGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc                                                                                       >  1:933231/4‑99 (MQ=255)                                                                                   tCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc                                                                                       <  2:933231/99‑1 (MQ=255)                                                                                     gTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc                                                                                       <  1:1268357/97‑1 (MQ=255)                                                                                     gTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc                                                                                       >  2:1268357/1‑97 (MQ=255)                                                                                     gTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTAcc                                             >  1:398683/1‑139 (MQ=255)                                                                                       ttATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCg                                                                                                                              >  2:646301/1‑56 (MQ=255)                                                                                       ttATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCg                                                                                                                              <  1:646301/56‑1 (MQ=255)                                                                                       ttATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc                                                                                       <  1:1410192/95‑1 (MQ=255)                                                                                       ttATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATc                                                                                       >  2:1410192/1‑95 (MQ=255)                                                                                       ttATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCtt                                           >  2:531246/1‑139 (MQ=255)                                                                                       ttATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCtt                                           >  1:1279691/1‑139 (MQ=255)                                                                                               cGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGa                                                                                                                            >  1:534937/1‑50 (MQ=255)                                                                                               cGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGa                                                                                                                            <  2:534937/50‑1 (MQ=255)                                                                                                 tCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACgg                                                           <  2:795313/113‑1 (MQ=255)                                                                                                 tCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACgg                                                           >  1:795313/1‑113 (MQ=255)                                                                                                 tCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAAt                                 >  1:462162/1‑139 (MQ=255)                                                                                                   aaTTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGata                                                                                         <  2:1324013/81‑1 (MQ=255)                                                                                                   aaTTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGata                                                                                         >  1:1324013/1‑81 (MQ=255)                                                                                                    aTTTTTTACCCGCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATAcc                              >  1:466849/1‑139 (MQ=255)                                                                                                               gCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAAt                                                                                                             <  1:775777/49‑1 (MQ=255)                                                                                                               gCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAAt                                                                                                             >  2:775777/1‑49 (MQ=255)                                                                                                               gCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGcc                                                                     >  2:105745/1‑89 (MQ=255)                                                                                                               gCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGcc                                                                     <  1:105745/89‑1 (MQ=255)                                                                                                               gCTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTg                   >  2:1436759/1‑139 (MQ=255)                                                                                                                cTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGa                  <  2:269501/139‑1 (MQ=255)                                                                                                                cTGAAAACCAGGTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGa                  <  2:1279691/139‑1 (MQ=255)                                                                                                                          ggTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTggg                                                 <  1:1534838/98‑1 (MQ=255)                                                                                                                          ggTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTggg                                                 >  2:1534838/1‑98 (MQ=255)                                                                                                                          ggTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCattga        >  1:114422/1‑139 (MQ=255)                                                                                                                           gTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCt                    >  1:1594922/1‑126 (MQ=255)                                                                                                                           gTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCt                    <  2:1594922/126‑1 (MQ=255)                                                                                                                           gTAGCCGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCattgat       >  1:1170417/1‑139 (MQ=255)                                                                                                                               ccGACTAAATGGTGCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGt   >  1:1074789/1‑139 (MQ=255)                                                                                                                               ccGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGcc                                                                     >  2:1054691/1‑73 (MQ=255)                                                                                                                               ccGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGcc                                                                     <  1:1054691/73‑1 (MQ=255)                                                                                                                                cGACTAAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGtt  >  2:1457861/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                      TGAAAATAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTT  >  NC_000913/2365152‑2365417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑