Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,414,699	T→C	intergenic (+27/‑48)	ackA → / → pta	acetate kinase A and propionate kinase 2/phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,414,699	0	T	C	100.0%	152.7 / NA	44	intergenic (+27/‑48)	ackA/pta	acetate kinase A and propionate kinase 2/phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/25);  total (19/25)

AATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGA  >  NC_000913/2414572‑2414829                                                                                                                                |                                                                                                                                   aaTCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTa                                                                                                                         <  2:1055030/139‑1 (MQ=255)  aTCTGGTTTCGTCAACAAAGAAGGTACCCGTCCTGCGATGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTaa                                                                                                                        <  2:1458262/139‑1 (MQ=255)           catcaACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCaaa                                                                                                               <  2:1165812/139‑1 (MQ=255)            atcaACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAAt                                                                                                              >  2:516991/1‑139 (MQ=255)                acaaAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATcggc                                                                                                          <  2:506076/139‑1 (MQ=255)                   aagaagGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGt                                                                                                       <  1:365335/139‑1 (MQ=255)                     gaagGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTaa                                                                                                     <  2:188121/139‑1 (MQ=255)                         gTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTaa                                                                                                     <  2:81048/135‑1 (MQ=255)                         gTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTaa                                                                                                     >  1:81048/1‑135 (MQ=255)                                tCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCt                                                                                                                                 <  2:738150/100‑1 (MQ=255)                                tCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCt                                                                                                                                 >  1:738150/1‑100 (MQ=255)                                tCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGAt                                                                                          >  1:289484/1‑139 (MQ=255)                                  cTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAag                                                                                               >  1:831079/1‑132 (MQ=255)                                  cTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAag                                                                                               <  2:831079/132‑1 (MQ=255)                                  cTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATaa                                                                                        >  2:161710/1‑139 (MQ=255)                                        tggtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCgtg                                                                                  <  1:161710/139‑1 (MQ=255)                                          gtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTc                                                                                <  1:516991/139‑1 (MQ=255)                                          gtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTc                                                                                <  1:59134/139‑1 (MQ=255)                                          gtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTc                                                                                >  2:327204/1‑139 (MQ=255)                                             aTCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                                             <  2:289484/139‑1 (MQ=255)                                                    ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGt                                                                                                       >  2:886724/1‑106 (MQ=255)                                                    ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGt                                                                                                       <  1:886724/106‑1 (MQ=255)                                                       acGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTc                                                                                <  1:240639/126‑1 (MQ=255)                                                       acGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTc                                                                                >  2:240639/1‑126 (MQ=255)                                                       acGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGCTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATg                                                                   <  1:285384/139‑1 (MQ=255)                                                        cGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAag                                                                                               >  1:85075/1‑110 (MQ=255)                                                        cGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAag                                                                                               <  2:85075/110‑1 (MQ=255)                                                        cGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTCACTGCCTGAATTCACACCACCAGCACAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCACGTATTATTATGc                                                                  >  1:103540/1‑139 (MQ=255)                                                        cGAAGAACTGGTAATCCGGCAAGACGCGAGCCCCCTGACTGCCTAATTTCACCCCGCCAGCTCACCTGGCGCCGCTGTTTTGTAACCCGCCAAATCGCCGGTAACGAAAGAGAATAAACCGTGTCCCGTATTATTATGc                                                                  >  2:1373006/1‑139 (MQ=255)                                                             aaCTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATc                                                             >  1:881755/1‑139 (MQ=255)                                                                  gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATcgg                                                                                                           >  1:1251392/1‑88 (MQ=255)                                                                  gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATcgg                                                                                                           <  2:1251392/88‑1 (MQ=255)                                                                  gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTAc                                                        >  2:285694/1‑139 (MQ=255)                                                                       cgcgCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaaa                                                                                                <  2:210225/94‑1 (MQ=255)                                                                       cgcgCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaaa                                                                                                >  1:210225/1‑94 (MQ=255)                                                                         cgcAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAAcc                                                 >  2:1225998/1‑139 (MQ=255)                                                                             aGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCg                                             <  1:1225998/139‑1 (MQ=255)                                                                                       cgccTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGAc                                   <  1:285694/139‑1 (MQ=255)                                                                                       cgccTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGAc                                   <  1:103769/139‑1 (MQ=255)                                                                                                  cTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGcc                        <  1:1373006/139‑1 (MQ=255)                                                                                                            acCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                                             >  1:593764/1‑76 (MQ=255)                                                                                                            acCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                                             <  2:593764/76‑1 (MQ=255)                                                                                                            acCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAt              <  1:327204/139‑1 (MQ=255)                                                                                                                        gctGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGa  >  2:312658/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                   AATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGA  >  NC_000913/2414572‑2414829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑