| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,451,573 | T→C | G4G (GGA→GGG) | yfcR ← | putative fimbrial‑like adhesin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,451,573 | 0 | T | C | 93.8% | 104.4 / 0.0 | 32 | coding (12/513 nt) | yfcR | putative fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (14/16); minor base . (1/1); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
TTGAACAGGGTGGCGGAGCATCGACCAACACGCGCAGAATTAAATCCACGGGTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGTAAGTCAGCACCGGTACGCTATCCATTTTCACA > NC_000913/2451440‑2451707 | ttgaACAGGGTGGCGGAGCATCGACCAACACGCGCAGAATTAAATCCACGGTTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACcccccc < 1:818147/139‑1 (MQ=255) gggTGGCGGAGCATCGACCAACACGCGCAGAATTAAATCCACGGGTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGtcatc < 1:268782/139‑1 (MQ=255) ggCGGAGCATCGACCAACACGCGCAGAATTAAATCCACGGGTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAAC‑CCCCCCGTCATCAGCCt > 2:1131266/1‑139 (MQ=255) cGGAGCATCGACCAACACGCGCAGAATTAAATCCACGGGTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCtt > 1:1169265/1‑139 (MQ=255) cATCGACCAACACGCGCAGAATTAAATCCACGGGTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAAcccccc > 1:727889/1‑120 (MQ=255) cATCGACCAACACGCGCAGAATTAAATCCACGGGTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAAcccccc < 2:727889/120‑1 (MQ=255) tCGACCAACACGCGCAGAATTAAATCCACGGGTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGgct < 1:444251/139‑1 (MQ=255) cAGAATTAAATCCACGGGTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCa > 2:762611/1‑139 (MQ=255) aCGGGTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACcccccc < 1:620918/92‑1 (MQ=255) aCGGGTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACcccccc > 2:620918/1‑92 (MQ=255) aCGGGTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTgg < 2:626163/131‑1 (MQ=255) aCGGGTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTgg > 1:626163/1‑131 (MQ=255) tttACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAAC‑CCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCAtt < 1:1131266/139‑1 (MQ=255) tactacCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTg < 2:1169265/139‑1 (MQ=255) actacCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGc < 1:762611/139‑1 (MQ=255) tAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGgaaga > 1:1278460/1‑130 (MQ=255) tAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGgaaga < 2:1278460/130‑1 (MQ=255) cTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCg < 2:426205/139‑1 (MQ=255) aGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGTCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTgcg > 2:1388614/1‑139 (MQ=255) cTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTg > 1:619821/1‑68 (MQ=255) cTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTg < 2:619821/68‑1 (MQ=255) tGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCa < 2:818602/103‑1 (MQ=255) tGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCa > 1:818602/1‑103 (MQ=255) tGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAg > 2:951746/1‑139 (MQ=255) tGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAg > 2:170948/1‑139 (MQ=255) cagcCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGt > 2:447084/1‑139 (MQ=255) gcCCTGCGCCCACTACAAACTTTTGACTCATAACcccccc < 2:375026/40‑1 (MQ=255) gcCCTGCGCCCACTACAAACTTTTGACTCATAACcccccc > 1:375026/1‑40 (MQ=255) gcCCTGCGCCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGTaa > 2:521632/1‑139 (MQ=255) gcCCACTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGTAAGTCAGCa < 1:1523505/139‑1 (MQ=255) aCTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGTAAGTCAGCACCgg > 2:727266/1‑139 (MQ=255) aCTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGTAAGTCAGCACCgg > 2:865050/1‑139 (MQ=255) cTACAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGTAAGTCAGCACCGGt < 1:447084/139‑1 (MQ=255) cAAACTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCAACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGTAAGTCAGCACCGGTACg < 1:951746/139‑1 (MQ=255) aaaCTTTTGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGTAAGTCAGCACCGGTACGc < 1:865050/139‑1 (MQ=255) tGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGTAAGTCAGCACCGGTACGCTATc < 2:1238767/136‑1 (MQ=255) tGACTCATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGTAAGTCAGCACCGGTACGCTATc > 1:1238767/1‑136 (MQ=255) cATAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGTAAGTCAGCACCGGTACGCTATCCATTTTca < 1:456950/139‑1 (MQ=255) tAACCCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGTAAGTCAGCACCGGTACGCTATCCATTTTcaca < 1:170948/139‑1 (MQ=255) | TTGAACAGGGTGGCGGAGCATCGACCAACACGCGCAGAATTAAATCCACGGGTTTACTACCAGCCATAGCAGATAAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGGCATCCGTGCGCCAAAGTCGTTGACGTAAGTCAGCACCGGTACGCTATCCATTTTCACA > NC_000913/2451440‑2451707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |