Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,525,930 C→T *309* (TAG→TAA yfeR ← transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,9300CT100.0% 186.1 / NA 58*309* (TAG→TAAyfeRtranscriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/30);  total (28/30)

ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGG  >  NC_000913/2525794‑2526059
                                                                                                                                        |                                                                                                                                 
caacatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTta                                                                                                                                 >  1:258332‑M2/133‑139 (MQ=255)
        cacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGAt                                                                                                                         >  2:333683‑M2/125‑139 (MQ=255)
         acattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATa                                                                                                                        <  1:113643‑M2/16‑1 (MQ=255)
             tactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAAc                                                                                                                    >  1:1467810‑M2/120‑139 (MQ=255)
               ctacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACgg                                                                                                                  >  2:710939‑M2/118‑139 (MQ=255)
               ctacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACgg                                                                                                                  >  1:40297‑M2/118‑139 (MQ=255)
                  cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc                                                                                                               >  2:135536‑M2/115‑139 (MQ=255)
                     ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtat                                                                                                                                >  2:561914‑M2/112‑119 (MQ=255)
                     ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtat                                                                                                                                <  1:561914‑M2/8‑1 (MQ=255)
                     ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATcccc                                                                                                            >  2:1200484‑M2/112‑139 (MQ=255)
                                     tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc                                                                                                                       >  2:1170914‑M2/96‑112 (MQ=255)
                                     tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc                                                                                                                       <  1:1170914‑M2/17‑1 (MQ=255)
                                       cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTc                                                                                                         >  1:217710‑M2/94‑124 (MQ=255)
                                       cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTc                                                                                                         <  2:217710‑M2/31‑1 (MQ=255)
                                           tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                      <  1:135536‑M2/50‑1 (MQ=255)
                                           tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                      <  2:1241755‑M2/50‑1 (MQ=255)
                                                 actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                <  1:1542040‑M2/56‑1 (MQ=255)
                                                    gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTatat                                                                                                                              <  2:656074‑M2/10‑1 (MQ=255)
                                                    gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTatat                                                                                                                              >  1:656074‑M2/81‑90 (MQ=255)
                                                     ggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGt                                                                            >  1:1006386‑M2/80‑139 (MQ=255)
                                                      gcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtc                                                                           >  2:281992‑M2/79‑139 (MQ=255)
                                                             ttttttccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                                    <  1:306327‑M2/68‑1 (MQ=255)
                                                             tttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                                    <  1:710939‑M2/68‑1 (MQ=255)
                                                               tttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAc                                                                  <  2:40297‑M2/70‑1 (MQ=255)
                                                                       gatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATa                                                          >  2:30154‑M2/62‑139 (MQ=255)
                                                                                  ctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                               <  2:1467810‑M2/89‑1 (MQ=255)
                                                                                  ctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCACATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                               <  2:258332‑M2/89‑1 (MQ=255)
                                                                                     ctccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                      <  1:9341‑M2/50‑1 (MQ=255)
                                                                                     ctccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                      >  2:9341‑M2/48‑97 (MQ=255)
                                                                                     ctccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggcg                                            >  2:1553961‑M2/48‑139 (MQ=255)
                                                                                      tccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc                                                                                                                             >  1:1251985‑M2/47‑57 (MQ=255)
                                                                                      tccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc                                                                                                                             <  2:1251985‑M2/11‑1 (MQ=255)
                                                                                        catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTg                                         >  1:221165‑M2/45‑139 (MQ=255)
                                                                                          tcaaatttaacttctgattgattactgccggggctcccggcaTACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                            <  2:862578‑M2/44‑1 (MQ=255)
                                                                                          tcaaatttaacttctgattgattactgccggggctcccggcaTACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                            >  1:862578‑M2/43‑86 (MQ=255)
                                                                                          tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTa                                                                                           <  1:1395930‑M2/45‑1 (MQ=255)
                                                                                          tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTa                                                                                           >  2:1395930‑M2/43‑87 (MQ=255)
                                                                                          tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                      <  1:577621‑M2/50‑1 (MQ=255)
                                                                                          tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                      >  2:577621‑M2/43‑92 (MQ=255)
                                                                                          tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAc                                                                     <  1:1554846‑M2/67‑1 (MQ=255)
                                                                                          tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAc                                                                     >  2:1554846‑M2/43‑109 (MQ=255)
                                                                                               tttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTgg                                                                             >  2:1256860‑M2/38‑96 (MQ=255)
                                                                                               tttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTgg                                                                             <  1:1256860‑M2/59‑1 (MQ=255)
                                                                                                ttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAGAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                 <  2:1066016‑M2/103‑1 (MQ=255)
                                                                                                ttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                 <  1:333683‑M2/103‑1 (MQ=255)
                                                                                                ttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                 <  1:1553961‑M2/103‑1 (MQ=255)
                                                                                                          attgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                 <  1:427620‑M2/103‑1 (MQ=255)
                                                                                                          attgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                 >  2:427620‑M2/27‑129 (MQ=255)
                                                                                                               ttactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcg                  <  2:221165‑M2/118‑1 (MQ=255)
                                                                                                                  ctgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAc               >  1:211058‑M2/19‑139 (MQ=255)
                                                                                                                    gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                <  2:94361‑M2/56‑1 (MQ=255)
                                                                                                                    gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                >  1:94361‑M2/17‑72 (MQ=255)
                                                                                                                            tcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAAc     <  1:281992‑M2/131‑1 (MQ=255)
                                                                                                                             cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                 <  2:1307978‑M2/103‑1 (MQ=255)
                                                                                                                             cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                 >  1:1307978‑M2/8‑110 (MQ=255)
                                                                                                                               cggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                 >  2:1188359‑M2/6‑76 (MQ=255)
                                                                                                                               cggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                 <  1:1188359‑M2/71‑1 (MQ=255)
                                                                                                                               cggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTgg  <  1:1355570‑M2/134‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                 
ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGG  >  NC_000913/2525794‑2526059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: