Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,591,603 G→A *119* (TAG→TAA yffB → putative ArsC family reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,591,6030GA100.0% 95.3 / NA 30*119* (TAG→TAAyffBputative ArsC family reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/17);  total (13/17)

CCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTGAA  >  NC_000913/2591476‑2591729
                                                                                                                               |                                                                                                                              
ccTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTg                                                                                                                     <  2:470565/139‑1 (MQ=255)
            cATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGa                                                                                                         <  1:1049224/139‑1 (MQ=255)
              ttAATGACTGAAATGCCTGCAATTATCAAACGTCCTTTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCGGGTTATTGAGc                                                                                                       >  1:223221/1‑139 (MQ=255)
              ttAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGc                                                                                                       <  2:692140/139‑1 (MQ=255)
              ttAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGc                                                                                                       >  1:692140/1‑139 (MQ=255)
                        aaaTGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACacaaca                                                                                             >  2:221707/1‑139 (MQ=255)
                                  aaTTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGacac                                                                                                 <  2:914447/125‑1 (MQ=255)
                                  aaTTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGacac                                                                                                 >  1:914447/1‑125 (MQ=255)
                                      aTCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCgccgcc                                                                               <  1:1606560/139‑1 (MQ=255)
                                                 aTTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGt                                                                    <  1:1322327/139‑1 (MQ=255)
                                                        tGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcgccgc                                                                                <  1:1044361/120‑1 (MQ=255)
                                                        tGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcgccgc                                                                                >  2:1044361/1‑120 (MQ=255)
                                                                    aaGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGcc                                                 <  2:588010/139‑1 (MQ=255)
                                                                          aTGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCtt                                           <  1:221707/139‑1 (MQ=255)
                                                                             ctgctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGcc                                                                                                                   >  1:584147/1‑64 (MQ=255)
                                                                             ctgctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGcc                                                                                                                   <  2:584147/64‑1 (MQ=255)
                                                                                ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTTCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGc                                                         <  2:859745/119‑1 (MQ=255)
                                                                                ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGAcaca                                                                                                <  1:351813/80‑1 (MQ=255)
                                                                                ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGAcaca                                                                                                >  2:351813/1‑80 (MQ=255)
                                                                                ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGc                                                         >  1:859745/1‑119 (MQ=255)
                                                                                     tttCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTAt                                                                                                            >  2:1538573/1‑63 (MQ=255)
                                                                                     tttCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTAt                                                                                                            <  1:1538573/63‑1 (MQ=255)
                                                                                     tttCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGaa                                >  1:536154/1‑139 (MQ=255)
                                                                                             aTTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCa                        <  1:614429/139‑1 (MQ=255)
                                                                                                   gTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGtt                                                                                                              <  2:513873/47‑1 (MQ=255)
                                                                                                   gTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGtt                                                                                                              >  1:513873/1‑47 (MQ=255)
                                                                                                         aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGc                                             >  2:1421029/1‑106 (MQ=255)
                                                                                                         aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGc                                             <  1:1421029/106‑1 (MQ=255)
                                                                                                                   ccATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTGaa  >  1:1532450/1‑139 (MQ=255)
                                                                                                                   ccATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTGaa  <  2:1116971/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                              
CCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTGAA  >  NC_000913/2591476‑2591729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: