Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,637,295 C→T R21H (CGT→CAT)  der ← GTPase, multicopy suppressor of ftsJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,637,2950CT100.0% 129.2 / NA 42R21H (CGT→CAT) derGTPase, multicopy suppressor of ftsJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/20);  total (22/20)

TCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGT  >  NC_000913/2637163‑2637408
                                                                                                                                    |                                                                                                                 
tcttctGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTaa                                                                                                             >  1:912542/1‑139 (MQ=255)
tcttctGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGGGCGAGTTAGATGGTTaa                                                                                                             >  1:68848/1‑139 (MQ=255)
      gtgCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACg                                                                                                       <  1:505879/139‑1 (MQ=255)
        gCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTg                                                                                                     <  2:855452/139‑1 (MQ=255)
          cATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCTTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGa                                                                                                   >  1:1285764/1‑139 (MQ=255)
                          aTCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAggg                                                                                   >  1:850610/1‑139 (MQ=255)
                                  aGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGAc                                                                           >  2:940604/1‑139 (MQ=255)
                                   gATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                  <  2:1561908/115‑1 (MQ=255)
                                   gATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                  >  1:1561908/1‑115 (MQ=255)
                                    aTAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACaa                                                                         >  2:166068/1‑139 (MQ=255)
                                         cTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAggg                                                                                   >  1:1266803/1‑124 (MQ=255)
                                         cTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAggg                                                                                   <  2:1266803/124‑1 (MQ=255)
                                           cACGGCCTTCAATTTCCGCACGACCTTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGAc                                                                  <  2:1285764/139‑1 (MQ=255)
                                              ggCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                              >  2:658484/1‑108 (MQ=255)
                                              ggCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                              <  1:658484/108‑1 (MQ=255)
                                              ggCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGAc                                                                  >  2:378028/1‑136 (MQ=255)
                                              ggCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGAc                                                                  <  1:378028/136‑1 (MQ=255)
                                                  ttCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGcgcg                                                                    >  2:731827/1‑130 (MQ=255)
                                                  ttCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGcgcg                                                                    <  1:731827/130‑1 (MQ=255)
                                                   tCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTa                                                          <  1:848766/139‑1 (MQ=255)
                                                     aaTTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTAcc                                                        <  2:912542/139‑1 (MQ=255)
                                                                            ggTCACAAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                  <  2:1375748/74‑1 (MQ=255)
                                                                            ggTCACAAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                  >  1:1375748/1‑74 (MQ=255)
                                                                                aCGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAatc                             >  2:1565465/1‑139 (MQ=255)
                                                                                 cGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTAcc                                                        <  1:1305928/111‑1 (MQ=255)
                                                                                 cGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTAcc                                                        >  2:1305928/1‑111 (MQ=255)
                                                                                         aCCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACaa                    >  1:267297/1‑139 (MQ=255)
                                                                                              ggAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAAc                                                                                                        >  1:532917/1‑50 (MQ=255)
                                                                                              ggAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAAc                                                                                                        <  2:532917/50‑1 (MQ=255)
                                                                                              ggAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACa                                                                          >  2:621431/1‑80 (MQ=255)
                                                                                              ggAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACa                                                                          <  1:621431/80‑1 (MQ=255)
                                                                                                   tCCGCAACCAGCGCCTCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCtat          >  1:1114381/1‑139 (MQ=255)
                                                                                                          ccAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTcgcg   <  1:940604/139‑1 (MQ=255)
                                                                                                           cAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGcgt  <  2:267297/139‑1 (MQ=255)
                                                                                                             gcgcATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                              <  2:653325/45‑1 (MQ=255)
                                                                                                             gcgcATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                              >  1:653325/1‑45 (MQ=255)
                                                                                                             gcgcATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGcgcg                                                                    >  1:795244/1‑71 (MQ=255)
                                                                                                             gcgcATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGcgcg                                                                    <  2:795244/71‑1 (MQ=255)
                                                                                                                        gTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGgcg                                                                                 >  2:1169852/1‑47 (MQ=255)
                                                                                                                        gTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGgcg                                                                                 <  1:1169852/47‑1 (MQ=255)
                                                                                                                             aGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGa                       >  2:1197046/1‑100 (MQ=255)
                                                                                                                             aGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGa                       <  1:1197046/100‑1 (MQ=255)
                                                                                                                                    |                                                                                                                 
TCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGT  >  NC_000913/2637163‑2637408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: