Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,685,910	G→A	A26T (GCC→ACC)	hmp →	fused nitric oxide dioxygenase/dihydropteridine reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,685,910	0	G	A	100.0%	195.1 / NA	61	A26T (GCC→ACC)	hmp	fused nitric oxide dioxygenase/dihydropteridine reductase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/29);  total (32/29)

CTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATATT  >  NC_000913/2685775‑2686038                                                                                                                                        |                                                                                                                                 cTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                               <  1:1536688/139‑1 (MQ=255)          ataAAATTCAATTTAGATACATCAATTAAGATGCAAAAAAAAGAAGACCATATGCTTGACGCTCAAACCATCGCTTCAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACg                                                                                                                      <  1:1244108/139‑1 (MQ=255)            aaGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGAc                                                                                                                    <  1:386384/139‑1 (MQ=255)              gATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                                  <  2:1261335/139‑1 (MQ=255)                       gagaTACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTa                                                                                                                        <  2:930440/124‑1 (MQ=255)                       gagaTACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTa                                                                                                                        >  1:930440/1‑124 (MQ=255)                             cATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                                      >  2:306399/1‑136 (MQ=255)                             cATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                                      <  1:306399/136‑1 (MQ=255)                                       gATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGt                                                                                                             <  1:1150006/119‑1 (MQ=255)                                       gATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGt                                                                                                             >  2:1150006/1‑119 (MQ=255)                                       gATGC‑AAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCa                                                                                        >  2:240814/1‑139 (MQ=255)                                        aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCa                                                                                        >  2:50466/1‑139 (MQ=255)                                        aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCa                                                                                        >  2:335890/1‑139 (MQ=255)                                           cAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                                            >  1:131169/1‑116 (MQ=255)                                           cAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                                            <  2:131169/116‑1 (MQ=255)                                             aaaaaaGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                               <  2:556700/95‑1 (MQ=255)                                             aaaaaaGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                               >  1:556700/1‑95 (MQ=255)                                                    gAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTC‑TAACCCAGAACTCAAAGAAATTTTTaa                                                                           <  1:689506/139‑1 (MQ=255)                                                     aaGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGAc                                                                                                                    >  2:1169410/1‑98 (MQ=255)                                                     aaGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGAc                                                                                                                    <  1:1169410/98‑1 (MQ=255)                                                     aaGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTaa                                                                           <  1:240814/139‑1 (MQ=255)                                                     aaGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTaa                                                                           <  2:725678/139‑1 (MQ=255)                                                          cATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGa                                                                      >  1:646680/1‑139 (MQ=255)                                                                         cAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATg                                                       <  2:1486204/139‑1 (MQ=255)                                                                           aaCCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                                            >  2:1468866/1‑84 (MQ=255)                                                                           aaCCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                                            <  1:1468866/84‑1 (MQ=255)                                                                               aTCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATc                                                 <  2:860793/139‑1 (MQ=255)                                                                                tCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                               <  1:578245/60‑1 (MQ=255)                                                                                tCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                               >  2:578245/1‑60 (MQ=255)                                                                                 cGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAACGTAATGGCGATCaa                                               >  1:1266227/1‑139 (MQ=255)                                                                                     aCAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTg                                           <  1:50466/139‑1 (MQ=255)                                                                                      cAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGa                                          >  1:53083/1‑139 (MQ=255)                                                                                       aGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGaa                                         >  1:700454/1‑139 (MQ=255)                                                                                            aaGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAAccc                                                                                                <  2:355034/79‑1 (MQ=255)                                                                                            aaGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAAccc                                                                                                >  1:355034/1‑79 (MQ=255)                                                                                                   caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTaa                                                                           >  2:348935/1‑93 (MQ=255)                                                                                                   caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAAc                             >  1:1431164/1‑139 (MQ=255)                                                                                                   caTCCCTTTACTGGTGGAAACGGGGCAAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTaa                                                                           <  1:348935/93‑1 (MQ=255)                                                                                                             cTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTaa                                                                           >  2:497324/1‑83 (MQ=255)                                                                                                             cTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTaa                                                                           <  1:497324/83‑1 (MQ=255)                                                                                                             cTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTgccg                   >  2:1451478/1‑139 (MQ=255)                                                                                                               ggtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTgccgcc                 >  1:182319/1‑139 (MQ=255)                                                                                                               ggtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTgccgcc                 <  2:1431164/139‑1 (MQ=255)                                                                                                                gtggAAACGGGGCCGAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                                      <  1:85539/53‑1 (MQ=255)                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                                      <  2:1416758/53‑1 (MQ=255)                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                                      >  1:1416758/1‑53 (MQ=255)                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                                      >  2:85539/1‑53 (MQ=255)                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                                 <  1:770823/90‑1 (MQ=255)                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                                 >  2:770823/1‑90 (MQ=255)                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCt                >  2:382214/1‑139 (MQ=255)                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCt                >  1:220602/1‑139 (MQ=255)                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCt                >  2:1284620/1‑139 (MQ=255)                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCt                >  1:781671/1‑139 (MQ=255)                                                                                                                 tggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                                      >  2:309851/1‑52 (MQ=255)                                                                                                                 tggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                                      <  1:309851/52‑1 (MQ=255)                                                                                                                 tggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATa                                                                                                    <  2:1020233/54‑1 (MQ=255)                                                                                                                 tggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATa                                                                                                    >  1:1020233/1‑54 (MQ=255)                                                                                                                  ggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTAc              <  2:182319/139‑1 (MQ=255)                                                                                                                  ggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTAc              >  1:303367/1‑139 (MQ=255)                                                                                                                    aaaCGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGc            <  1:382214/139‑1 (MQ=255)                                                                                                                              aaaGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATAtt  >  1:874731/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                 CTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATATT  >  NC_000913/2685775‑2686038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑