Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	255,659	A→G	C20R (TGT→CGT)	pepD ←	aminoacyl‑histidine dipeptidase (peptidase D)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	255,659	0	A	G	100.0%	317.9 / NA	87	C20R (TGT→CGT)	pepD	aminoacyl‑histidine dipeptidase (peptidase D)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/42);  total (45/42)

CGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACAGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGA  >  NC_000913/255530‑255793                                                                                                                                  |                                                                                                                                       cGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               >  1:589767/1‑139 (MQ=255) cGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               <  2:1263032/139‑1 (MQ=255)        aGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAata                                                                                                                        >  1:700134/1‑139 (MQ=255)           tttACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATcc                                                                                                                     <  2:700134/139‑1 (MQ=255)             tACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCca                                                                                                                   >  2:784576/1‑139 (MQ=255)                       gATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCtgt                                                                                                         <  1:784576/139‑1 (MQ=255)                                 acctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                >  2:1264286/1‑122 (MQ=255)                                 acctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                <  1:1264286/122‑1 (MQ=255)                                 acctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAAtt                                                                                               >  1:1348797/1‑139 (MQ=255)                                 acctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAAtt                                                                                               >  1:1415395/1‑139 (MQ=255)                                    tGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgag                                                                                            <  2:307665/139‑1 (MQ=255)                                     gATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  1:776922/129‑1 (MQ=255)                                     gATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     >  2:776922/1‑129 (MQ=255)                                     gATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           <  2:1348797/139‑1 (MQ=255)                                      aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGAc                                                                                          >  2:1071198/1‑139 (MQ=255)                                        cgcgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             >  1:1438275/1‑118 (MQ=255)                                        cgcgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             <  2:1438275/118‑1 (MQ=255)                                        cgcgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     >  2:1587493/1‑126 (MQ=255)                                        cgcgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  1:1587493/126‑1 (MQ=255)                                             tCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                <  2:415472/110‑1 (MQ=255)                                             tCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                >  1:415472/1‑110 (MQ=255)                                              cGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGa                                                                                  <  1:807956/139‑1 (MQ=255)                                                                    tGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  1:268876/98‑1 (MQ=255)                                                                    tGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     >  2:268876/1‑98 (MQ=255)                                                                           ccaacAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAatat                                                                                                                       >  2:442671/1‑73 (MQ=255)                                                                           ccaacAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAatat                                                                                                                       <  1:442671/73‑1 (MQ=255)                                                                                   gTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             <  1:31991/75‑1 (MQ=255)                                                                                   gTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             >  2:31991/1‑75 (MQ=255)                                                                                    tATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCaa                                                   >  2:723085/1‑132 (MQ=255)                                                                                    tATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCaa                                                   <  1:723085/132‑1 (MQ=255)                                                                                    tATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTa                                            >  2:1116181/1‑139 (MQ=255)                                                                                     aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAatat                                                                                                                       >  1:851812/1‑63 (MQ=255)                                                                                     aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAatat                                                                                                                       <  2:851812/63‑1 (MQ=255)                                                                                     aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCtgt                                                                                                         <  1:1483595/77‑1 (MQ=255)                                                                                     aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCtgt                                                                                                         >  2:1483595/1‑77 (MQ=255)                                                                                     aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTAt                                           >  2:685462/1‑139 (MQ=255)                                                                                      ttCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTAtt                                          >  1:900885/1‑139 (MQ=255)                                                                                       tCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTg                                         <  1:1071198/139‑1 (MQ=255)                                                                                        cAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGc                                        <  2:1415395/139‑1 (MQ=255)                                                                                         agggAGTTGCTGTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCATAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCa                                       >  1:933471/4‑139 (MQ=255)                                                                                         aGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCa                                       >  2:1288268/1‑139 (MQ=255)                                                                                         aGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCa                                       >  1:1186426/1‑139 (MQ=255)                                                                                          gCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           <  1:1451394/118‑1 (MQ=255)                                                                                          gCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           >  2:1451394/1‑118 (MQ=255)                                                                                              gTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                >  2:1130070/1‑61 (MQ=255)                                                                                              gTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                <  1:1130070/61‑1 (MQ=255)                                                                                              gTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             >  1:148733/1‑64 (MQ=255)                                                                                              gTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             <  2:148733/64‑1 (MQ=255)                                                                                              gTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTTCTTGTCGATCACCCGCAAAACAGTATTGc                                        >  2:974380/1‑133 (MQ=255)                                                                                              gTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGc                                        <  1:974380/133‑1 (MQ=255)                                                                                              gTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTca                                  >  1:882815/1‑139 (MQ=255)                                                                                                tGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTcaca                                >  2:529072/1‑139 (MQ=255)                                                                                                            tAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCt                                                                                                           <  1:1280883/52‑1 (MQ=255)                                                                                                            tAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCt                                                                                                           >  2:1280883/1‑52 (MQ=255)                                                                                                            tAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCAcc                                                        <  2:1349327/103‑1 (MQ=255)                                                                                                            tAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCAcc                                                        >  1:1349327/1‑103 (MQ=255)                                                                                                            tAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGaa                    >  1:782063/1‑139 (MQ=255)                                                                                                             aGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGaaa                   <  2:360883/139‑1 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCg                                                                                                              <  2:1087261/46‑1 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCg                                                                                                              >  1:1087261/1‑46 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGt                                                                        >  2:292893/1‑84 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGt                                                                        <  1:292893/84‑1 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           >  2:964157/1‑97 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           <  1:964157/97‑1 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCg                                                      <  2:1505265/102‑1 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCg                                                      >  1:1505265/1‑102 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAt                 >  1:1592247/1‑139 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     >  2:1516296/1‑53 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  1:1516296/53‑1 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           >  1:1258263/1‑63 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           <  2:1258263/63‑1 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGc                                        <  2:1502542/111‑1 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGc                                        <  1:200658/111‑1 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGc                                        >  2:200658/1‑111 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGc                                        >  1:1502542/1‑111 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgttgt            <  2:933471/139‑1 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgttgt            <  1:1288268/139‑1 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgttgt            <  2:782063/139‑1 (MQ=255)                                                                                                                       aGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCaa         >  2:501862/1‑139 (MQ=255)                                                                                                                         gAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAaca       <  2:1574259/139‑1 (MQ=255)                                                                                                                         gAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAaca       <  1:529072/139‑1 (MQ=255)                                                                                                                         gAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAaca       <  2:589767/139‑1 (MQ=255)                                                                                                                              gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAt                 >  1:408122/1‑124 (MQ=255)                                                                                                                              gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAt                 <  2:408122/124‑1 (MQ=255)                                                                                                                              gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGa  >  2:821657/1‑139 (MQ=255)                                                                                                                               aaCGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCg                                                      <  1:390611/86‑1 (MQ=255)                                                                                                                               aaCGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCg                                                      >  2:390611/1‑86 (MQ=255)                                                                                                                                  |                                                                                                                                       CGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACAGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGA  >  NC_000913/255530‑255793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑