Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,796,337	C→T	*150* (TAG→TAA)	ygaU ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,796,337	0	C	T	100.0%	168.1 / NA	52	*150* (TAG→TAA)	ygaU	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/25);  total (27/25)

GGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGG  >  NC_000913/2796201‑2796461                                                                                                                                         |                                                                                                                             ggaggaTAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAattaatta                                                                                                                            <  2:1264927/139‑1 (MQ=255) ggaggaTAAACACGATCAGCACCAGACCCTGACTGCAGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAattaatta                                                                                                                            <  2:100888/139‑1 (MQ=255)       tAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTc                                                                                                                      >  1:1366332/1‑139 (MQ=255)               aTCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGaa                                                                                                                 <  2:1324600/136‑1 (MQ=255)               aTCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGaa                                                                                                                 >  1:1324600/1‑136 (MQ=255)                 cAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGc                                                                                                            >  2:1489403/1‑139 (MQ=255)                          aCCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCACTTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGc                                                                                                   >  2:971516/1‑139 (MQ=255)                          aCCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTActc                                                                                                                         >  2:745904/1‑117 (MQ=255)                          aCCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTActc                                                                                                                         <  1:745904/117‑1 (MQ=255)                          aCCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGc                                                                                                   >  1:1394504/1‑139 (MQ=255)                              tGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCg                                                                                               >  1:579698/1‑139 (MQ=255)                                 cTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGAt                                                                                            <  2:1366332/139‑1 (MQ=255)                                      gcggTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAattaatta                                                                                                                            >  1:773312/1‑102 (MQ=255)                                      gcggTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAattaatta                                                                                                                            <  2:1365653/102‑1 (MQ=255)                                      gcggTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAAtt                                                                                       <  2:579698/139‑1 (MQ=255)                                          tACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTAt                                                                                   <  1:1489403/139‑1 (MQ=255)                                                          cgcgcgcgCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCAt                                                                   >  2:342423/1‑139 (MQ=255)                                                              cgcgcCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTActct                                                                                                                        >  1:366840/1‑82 (MQ=255)                                                              cgcgcCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTActct                                                                                                                        <  2:366840/82‑1 (MQ=255)                                                                    aGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCAt                                                                   >  2:664745/1‑129 (MQ=255)                                                                    aGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCAt                                                                   <  1:664745/129‑1 (MQ=255)                                                                                  ccAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATa                                                                                                               >  1:932748/1‑71 (MQ=255)                                                                                  ccAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATa                                                                                                               <  2:932748/71‑1 (MQ=255)                                                                                  ccAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGAtttt                                           >  1:1527743/1‑139 (MQ=255)                                                                                               aCGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGa                                               >  1:367099/1‑122 (MQ=255)                                                                                               aCGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGa                                               <  2:367099/122‑1 (MQ=255)                                                                                                 gCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGa                                                  >  2:9823/1‑117 (MQ=255)                                                                                                 gCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGa                                                  <  1:9823/117‑1 (MQ=255)                                                                                                 gCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGa                                               >  1:1530392/1‑120 (MQ=255)                                                                                                 gCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGa                                               <  2:1530392/120‑1 (MQ=255)                                                                                                 gCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGc                            >  1:1608444/1‑139 (MQ=255)                                                                                                   cAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGt                          <  1:1404127/139‑1 (MQ=255)                                                                                                   cAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGt                          <  2:1527743/139‑1 (MQ=255)                                                                                                             tATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGAc                >  1:155956/1‑139 (MQ=255)                                                                                                               tCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCt              >  1:1134733/1‑139 (MQ=255)                                                                                                                  agcagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGt                          >  2:485386/1‑124 (MQ=255)                                                                                                                  agcagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGt                          <  1:485386/124‑1 (MQ=255)                                                                                                                    cagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGa                                               >  1:73556/1‑101 (MQ=255)                                                                                                                    cagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGa                                               <  2:73556/101‑1 (MQ=255)                                                                                                                    cagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGAtt                                             <  1:1083751/103‑1 (MQ=255)                                                                                                                    cagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGAtt                                             >  2:1083751/1‑103 (MQ=255)                                                                                                                       gCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGa                                               >  2:107276/1‑98 (MQ=255)                                                                                                                       gCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGa                                               <  1:107276/98‑1 (MQ=255)                                                                                                                       gCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTa                                          <  2:1459705/103‑1 (MQ=255)                                                                                                                       gCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTa                                          >  1:1459705/1‑103 (MQ=255)                                                                                                                         gATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCg                                                                                <  2:1195221/63‑1 (MQ=255)                                                                                                                         gATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCg                                                                                >  1:1195221/1‑63 (MQ=255)                                                                                                                         gATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGAc                >  2:1610901/1‑127 (MQ=255)                                                                                                                         gATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGAc                <  1:1610901/127‑1 (MQ=255)                                                                                                                         gATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAAt    <  2:1608444/139‑1 (MQ=255)                                                                                                                         gATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAAt    >  1:23587/1‑139 (MQ=255)                                                                                                                          aTGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATg   <  2:339533/139‑1 (MQ=255)                                                                                                                           tggctggcAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATgg  <  2:23587/139‑1 (MQ=255)                                                                                                                           tggctggcAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATgg  <  2:1134733/139‑1 (MQ=255)                                                                                                                                 gcAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGt                          >  2:1539745/1‑109 (MQ=255)                                                                                                                                 gcAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGt                          <  1:1539745/109‑1 (MQ=255)                                                                                                                                         |                                                                                                                             GGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGG  >  NC_000913/2796201‑2796461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑