Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,827,351 G→A *320* (TAG→TAA)  srlE → glucitol/sorbitol‑specific enzyme IIB component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,827,3510GA100.0% 148.5 / NA 47*320* (TAG→TAA) srlEglucitol/sorbitol‑specific enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/23);  total (24/23)

GTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTA  >  NC_000913/2827217‑2827477
                                                                                                                                      |                                                                                                                              
gtcggtTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGc                                                                                                                            >  1:1249662/1‑139 (MQ=255)
gtcggtTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGc                                                                                                                            >  2:1334156/1‑139 (MQ=255)
       tGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACa                                                                                                                     >  1:1032758/1‑139 (MQ=255)
               gCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGtt                                                                                                             >  2:335439/1‑139 (MQ=255)
                                             ggTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACAt                                                                                                                    >  1:1241817/1‑102 (MQ=255)
                                             ggTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACAt                                                                                                                    <  2:1241817/102‑1 (MQ=255)
                                               tgtCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGgcgc                                                                             >  1:329785/1‑139 (MQ=255)
                                                           aCTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGa                                                                 >  2:1332064/1‑139 (MQ=255)
                                                               gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGcc                                                             >  1:548688/1‑139 (MQ=255)
                                                                 gAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCt                                                           <  1:675697/139‑1 (MQ=255)
                                                                  aGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCg                                                                                                               <  1:951830/86‑1 (MQ=255)
                                                                  aGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCg                                                                                                               >  2:951830/1‑86 (MQ=255)
                                                                    ccGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGAc                                                                                                                 <  1:311266/82‑1 (MQ=255)
                                                                    ccGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGAc                                                                                                                 >  2:311266/1‑82 (MQ=255)
                                                                    ccGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAg                                                        >  2:1465453/1‑139 (MQ=255)
                                                                    ccGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAg                                                        >  2:875811/1‑139 (MQ=255)
                                                                    ccGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGACTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGACGCGAGTCCCATTGACGCCCTCAg                                                        >  1:420175/1‑139 (MQ=255)
                                                                        tttttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGAc                                                    <  1:875811/139‑1 (MQ=255)
                                                                         ttttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGAc                                                                                                                 >  1:472857/1‑77 (MQ=255)
                                                                         ttttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGAc                                                                                                                 <  2:472857/77‑1 (MQ=255)
                                                                         ttttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGAcc                                                   <  1:1334156/139‑1 (MQ=255)
                                                                             aaCCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCGGTGACCAGAt                                               <  1:1332064/139‑1 (MQ=255)
                                                                                       ccGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCAcc                                     <  2:1249662/139‑1 (MQ=255)
                                                                                                  gATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATc                                                                                           <  1:1497605/74‑1 (MQ=255)
                                                                                                  gATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATc                                                                                           >  2:1497605/1‑74 (MQ=255)
                                                                                                  gATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCAc                                                                                         >  1:708484/1‑76 (MQ=255)
                                                                                                  gATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCAc                                                                                         <  2:708484/76‑1 (MQ=255)
                                                                                                      gCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGaccacc                                                                                              <  1:939234/67‑1 (MQ=255)
                                                                                                      gCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGaccacc                                                                                              >  2:939234/1‑67 (MQ=255)
                                                                                                      gCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAg                                                                          <  1:1215755/87‑1 (MQ=255)
                                                                                                      gCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAg                                                                          >  2:1215755/1‑87 (MQ=255)
                                                                                                      gCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgcgcg                      >  2:1391275/1‑139 (MQ=255)
                                                                                                        cTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCt                                                           >  1:1086221/1‑100 (MQ=255)
                                                                                                        cTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCt                                                           <  2:1086221/100‑1 (MQ=255)
                                                                                                             ttGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCgg               <  2:329785/139‑1 (MQ=255)
                                                                                                             ttGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCgg               <  1:335439/139‑1 (MQ=255)
                                                                                                                tctcCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGAc                                                    <  1:872951/99‑1 (MQ=255)
                                                                                                                tctcCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGAc                                                    >  2:872951/1‑99 (MQ=255)
                                                                                                                  tcCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACg                                                               >  2:1347371/1‑86 (MQ=255)
                                                                                                                  tcCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACg                                                               <  1:1347371/86‑1 (MQ=255)
                                                                                                                     ggTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGaa       <  2:420175/139‑1 (MQ=255)
                                                                                                                          tatctatcAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTa  <  2:294711/139‑1 (MQ=255)
                                                                                                                          tatctatcAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTa  <  1:1391275/139‑1 (MQ=255)
                                                                                                                                tcAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGa                                                                           <  2:79579/60‑1 (MQ=255)
                                                                                                                                tcAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGa                                                                           >  1:79579/1‑60 (MQ=255)
                                                                                                                                tcAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCg         <  2:1506069/126‑1 (MQ=255)
                                                                                                                                tcAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCg         >  1:1506069/1‑126 (MQ=255)
                                                                                                                                      |                                                                                                                              
GTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTA  >  NC_000913/2827217‑2827477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑