Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,842,414	G→A	*475* (TAG→TAA)	ascB →	cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,842,414	0	G	A	100.0%	103.4 / NA	33	*475* (TAG→TAA)	ascB	cryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/16);  total (17/16)

AATGAGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCC  >  NC_000913/2842282‑2842548                                                                                                                                       |                                                                                                                                       aaTGAGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACa                                                                                                                                  >  2:1464916/1‑139 (MQ=255)  aTGAGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAg                                                                                                                                 >  2:849523/1‑139 (MQ=255)                cGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATgcggcg                                                                                                                   >  1:1605544/1‑139 (MQ=255)                  gCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTg                                                                                                                 <  2:674814/139‑1 (MQ=255)                  gCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTg                                                                                                                 <  1:849523/139‑1 (MQ=255)                        tctttgtTGACCGTGACGACGCAGGCAACGGTACGCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGcc                                                                                                           >  1:979074/1‑139 (MQ=255)                            tgttgACCGTGACGACGCAGGCAACGGTACGCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                       <  2:979074/139‑1 (MQ=255)                            tgttgACCGTGACGACGCAGGCAACGGTACGCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                       <  2:1605544/139‑1 (MQ=255)                                gACCGTGACGACGCAGGCAACGGTACGCTGACGCGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                     <  1:783433/139‑1 (MQ=255)                                             cAGGCAACGGTACGCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                      >  2:670461/1‑139 (MQ=255)                                                 cAACGGTACGCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGaa                                                                                  <  2:1153740/139‑1 (MQ=255)                                                          gCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAAc                                                                                                                                   >  2:671501/1‑81 (MQ=255)                                                          gCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAAc                                                                                                                                   <  1:671501/81‑1 (MQ=255)                                                             gA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAg                                                                          >  2:272713/1‑135 (MQ=255)                                                             gA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAg                                                                          <  1:272713/135‑1 (MQ=255)                                                                                          gtggtATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAgtaagtaa                                                                                                                                    >  2:1106939/1‑50 (MQ=255)                                                                                          gtggtATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAgtaagtaa                                                                                                                                    <  1:1106939/50‑1 (MQ=255)                                                                                          gtggtATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTa                                           >  2:155390/1‑139 (MQ=255)                                                                                              tataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                                  >  2:1104511/1‑128 (MQ=255)                                                                                              tataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCggg                                                  <  1:1104511/128‑1 (MQ=255)                                                                                                taAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCAttt                                     <  1:404556/139‑1 (MQ=255)                                                                                                taAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCAttt                                     >  2:404556/1‑139 (MQ=255)                                                                                                          aTTGCCAGTAATGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCACATCGGGCAATTTAGCATTTGGCATCagag                           <  1:670461/139‑1 (MQ=255)                                                                                                                    aTGGGGAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCAcc                 <  1:155390/139‑1 (MQ=255)                                                                                                                        ggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                 >  1:111909/1‑55 (MQ=255)                                                                                                                        ggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                 <  2:111909/55‑1 (MQ=255)                                                                                                                        ggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                      <  1:1578065/66‑1 (MQ=255)                                                                                                                        ggAAGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCa                                                                                      >  2:1578065/1‑66 (MQ=255)                                                                                                                          aaGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCAtt                                      >  1:1529753/1‑112 (MQ=255)                                                                                                                          aaGATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCAtt                                      <  2:1529753/112‑1 (MQ=255)                                                                                                                            gATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAg         >  2:1589952/1‑139 (MQ=255)                                                                                                                            gATTTAGAGTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAg         >  2:476822/1‑139 (MQ=255)                                                                                                                                 agagTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGaa       <  2:82810/136‑1 (MQ=255)                                                                                                                                 agagTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGaa       >  1:82810/1‑136 (MQ=255)                                                                                                                                  gagTAAGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATc   >  1:1545571/1‑139 (MQ=255)                                                                                                                                   agtaagtaACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATcc  <  1:2820/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AATGAGCAAACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGA‑‑CGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCC  >  NC_000913/2842282‑2842548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑