Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 284,869 C→T P557S (CCG→TCG)  yagF → CP4‑6 prophage, dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913284,8690CT100.0% 150.7 / NA 48P557S (CCG→TCG) yagFCP4‑6 prophage, dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/25);  total (23/25)

ACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGCCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCG  >  NC_000913/284734‑284990
                                                                                                                                       |                                                                                                                         
aCCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGa                                                                                                                        <  1:997680/139‑1 (MQ=255)
           cACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTg                                                                                                             >  2:795316/1‑139 (MQ=255)
            aCCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTgc                                                                                                            <  1:795316/139‑1 (MQ=255)
            aCCTCCGCGCT‑AAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTgcg                                                                                                           <  1:1313165/139‑1 (MQ=255)
             ccTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTgcg                                                                                                           <  2:1405449/139‑1 (MQ=255)
                   cgcTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATaa                                                                                                     >  2:1372353/1‑139 (MQ=255)
                   cgcTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATaa                                                                                                     >  1:524101/1‑139 (MQ=255)
                                ctcGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgag                                                                                        >  1:160301/1‑139 (MQ=255)
                                 tcGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                       <  2:160301/139‑1 (MQ=255)
                                 tcGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                       <  1:1372353/139‑1 (MQ=255)
                                 tcGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                       <  2:402783/139‑1 (MQ=255)
                                 tcGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                       <  2:524101/139‑1 (MQ=255)
                                 tcGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                       <  1:757657/139‑1 (MQ=255)
                                    tGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTg                                                                                    >  2:50169/1‑139 (MQ=255)
                                     ggggCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACg                                                                                                   >  2:976051/1‑123 (MQ=255)
                                     ggggCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                       <  1:1119813/135‑1 (MQ=255)
                                     ggggCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                       >  2:1119813/1‑135 (MQ=255)
                                     ggggCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGAGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACg                                                                                                   <  1:976051/123‑1 (MQ=255)
                                                 tgtCGCTCATCACCGATGCGCGCTTCTCGGG‑GTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCg                                                                                 >  2:1384546/1‑128 (MQ=255)
                                                 tgtCGCTCATCACCGATGCGCGCTTCTCGGG‑GTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCg                                                                                 <  1:1384546/128‑1 (MQ=255)
                                                                     cgcTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGa                                                   <  2:1062671/139‑1 (MQ=255)
                                                                          ctcGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGAc                                                                                                 >  1:1355182/1‑88 (MQ=255)
                                                                          ctcGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGAc                                                                                                 <  2:1355182/88‑1 (MQ=255)
                                                                          ctcGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTc                                              >  2:269826/1‑139 (MQ=255)
                                                                                  tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGa                                                                     >  2:1560252/1‑108 (MQ=255)
                                                                                  tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGa                                                                     <  1:1560252/108‑1 (MQ=255)
                                                                                  tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGc                                                       <  1:570227/122‑1 (MQ=255)
                                                                                  tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGc                                                       >  2:570227/1‑122 (MQ=255)
                                                                                  tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAAc                                                 >  2:323298/1‑128 (MQ=255)
                                                                                  tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAAc                                                 <  1:323298/128‑1 (MQ=255)
                                                                                          ggCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGTGATTGCCGTGGATCGTc                                                                        >  1:975621/1‑97 (MQ=255)
                                                                                          ggCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTc                                                                        <  2:975621/97‑1 (MQ=255)
                                                                                              ccTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGAcatcat                                                                                            >  1:1362756/1‑73 (MQ=255)
                                                                                              ccTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGAcatcat                                                                                            <  2:1362756/73‑1 (MQ=255)
                                                                                                 gCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCt                       >  1:176815/1‑139 (MQ=255)
                                                                                                  tttCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                       >  1:1007838/2‑74 (MQ=255)
                                                                                                  cTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGTTAACGACATCATCgaga                                                                                       <  2:1007838/74‑1 (MQ=255)
                                                                                                         cACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                       >  2:1041843/1‑67 (MQ=255)
                                                                                                         cACGTGTCGCCGGAGGCACTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                       <  1:1041843/67‑1 (MQ=255)
                                                                                                           cGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGa                                                                                                  >  2:1614822/1‑54 (MQ=255)
                                                                                                           cGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGa                                                                                                  <  1:1614822/54‑1 (MQ=255)
                                                                                                            gtgtCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGAc                                                                                                 <  2:1559777/54‑1 (MQ=255)
                                                                                                            gtgtCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGAc                                                                                                 >  1:1559777/1‑54 (MQ=255)
                                                                                                                      aGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGgcgcgcg  >  1:876265/1‑139 (MQ=255)
                                                                                                                         cgcTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCg                                          >  2:1475244/1‑96 (MQ=255)
                                                                                                                         cgcTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCg                                          <  1:1475244/96‑1 (MQ=255)
                                                                                                                           cTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGa                                                                             >  1:830027/1‑59 (MQ=255)
                                                                                                                           cTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGa                                                                             <  2:830027/59‑1 (MQ=255)
                                                                                                                                 ggcgggtCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTg                                                                               <  1:758473/51‑1 (MQ=255)
                                                                                                                                 ggcgggtCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTg                                                                               >  2:758473/1‑51 (MQ=255)
                                                                                                                                       |                                                                                                                         
ACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGCCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCG  >  NC_000913/284734‑284990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑