Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,970,351	G→A	*230* (TAG→TAA)	mutH →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,970,351	0	G	A	100.0%	117.2 / NA	38	*230* (TAG→TAA)	mutH	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/19);  total (19/19)

GCAGCGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACG  >  NC_000913/2970220‑2970476                                                                                                                                    |                                                                                                                              gcagcGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGc                                                                                                                        >  1:1547189/1‑139 (MQ=255)                      aCCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   <  2:1547189/139‑1 (MQ=255)                      aCCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   <  2:248186/139‑1 (MQ=255)                                 tGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGc                                                                                                  <  2:477928/129‑1 (MQ=255)                                 tGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGc                                                                                                  >  1:477928/1‑129 (MQ=255)                                           ggCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTc                                                                                                       <  2:768881/114‑1 (MQ=255)                                           ggCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTc                                                                                                       >  1:768881/1‑114 (MQ=255)                                                         gACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAg                                                                     >  1:1539385/1‑134 (MQ=255)                                                         gACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAg                                                                     <  2:1539385/134‑1 (MQ=255)                                                                          tttATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCa                                               <  2:577158/139‑1 (MQ=255)                                                                             aTTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATgg                                            >  1:143061/1‑139 (MQ=255)                                                                                  aagaagaaTTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTc                                                                                  >  1:396561/1‑96 (MQ=255)                                                                                  aagaagaaTTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTc                                                                                  <  2:396561/96‑1 (MQ=255)                                                                                                   tGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCt                                                                                                                       <  2:1069728/42‑1 (MQ=255)                                                                                                   tGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCt                                                                                                                       >  1:1069728/1‑42 (MQ=255)                                                                                                     cACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   <  1:1445925/60‑1 (MQ=255)                                                                                                     cACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   >  2:1445925/1‑60 (MQ=255)                                                                                                       ctactGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTgc                  <  2:143061/139‑1 (MQ=255)                                                                                                          ctGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAggg                                                                   <  2:181296/87‑1 (MQ=255)                                                                                                          ctGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAggg                                                                   >  1:181296/1‑87 (MQ=255)                                                                                                             gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   >  2:1562615/1‑52 (MQ=255)                                                                                                             gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   <  2:853921/52‑1 (MQ=255)                                                                                                             gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   >  1:853921/1‑52 (MQ=255)                                                                                                             gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   <  1:1562615/52‑1 (MQ=255)                                                                                                             gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAggg                                                                   >  1:780512/1‑84 (MQ=255)                                                                                                             gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAggg                                                                   <  2:780512/84‑1 (MQ=255)                                                                                                             gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGc                                                <  2:109966/103‑1 (MQ=255)                                                                                                             gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGc                                                >  1:109966/1‑103 (MQ=255)                                                                                                             gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCgg            >  2:514084/1‑139 (MQ=255)                                                                                                             gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCg             >  2:1454578/1‑138 (MQ=255)                                                                                                             gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCg             <  1:1454578/138‑1 (MQ=255)                                                                                                                  tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgc       >  2:1446855/1‑139 (MQ=255)                                                                                                                  tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTACCGGGCATAGAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgc       >  1:995127/1‑139 (MQ=255)                                                                                                                       ttCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCtt                                                                                   >  1:676716/1‑58 (MQ=255)                                                                                                                       ttCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCtt                                                                                   <  2:676716/58‑1 (MQ=255)                                                                                                                       ttCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATCTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgctgacg  <  2:995127/139‑1 (MQ=255)                                                                                                                                 gTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTc              >  2:140742/1‑117 (MQ=255)                                                                                                                                 gTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTc              <  1:140742/117‑1 (MQ=255)                                                                                                                                    |                                                                                                                              GCAGCGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACG  >  NC_000913/2970220‑2970476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑