Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,411	C→T	*394* (TAG→TAA)	yqeF ←	short chain acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,411	0	C	T	100.0%	251.6 / NA	77	*394* (TAG→TAA)	yqeF	short chain acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/39);  total (38/39)

AGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAG  >  NC_000913/2984275‑2984538                                                                                                                                         |                                                                                                                                aGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTa                                                                                                                               >  1:1216817/1‑139 (MQ=255)  gTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTAt                                                                                                                              >  2:965153/1‑139 (MQ=255)     cAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCg                                                                                                                           <  2:431825/139‑1 (MQ=255)             aTCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTc                                                                                                                   >  2:1518464/1‑139 (MQ=255)                    atatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTc                                                                                                            >  2:787372/1‑139 (MQ=255)                      atGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCaa                                                                                                          <  2:1216817/139‑1 (MQ=255)                        gTCATGAGAATGGAAAAATGAGGGAGCCCAGTAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATg                                                                                                        >  2:860184/1‑139 (MQ=255)                          cATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCTACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                      <  1:965153/139‑1 (MQ=255)                           aTGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCca                                                                                                     <  1:1518464/139‑1 (MQ=255)                              agaATGGAAAAATGAGGGAGCCCAGAAAATGCATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCacac                                                                                                  >  2:356451/1‑139 (MQ=255)                                    gAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGc                                                                                            <  2:492818/139‑1 (MQ=255)                                     aaaaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                           <  1:156240/139‑1 (MQ=255)                                     aaaaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                           <  2:245502/139‑1 (MQ=255)                                                     agtaaaTGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGc                                                                           <  1:860184/136‑1 (MQ=255)                                                            gtatgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCa                                                                    <  1:1414486/139‑1 (MQ=255)                                                               tgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTa                                                                                                                               >  1:1417191/1‑77 (MQ=255)                                                                gtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcccc                                                                                         >  1:1428348/1‑114 (MQ=255)                                                                gtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcccc                                                                                         <  2:1428348/114‑1 (MQ=255)                                                                 tCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGc                                                                      <  2:947611/132‑1 (MQ=255)                                                                 tCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGc                                                                      >  1:947611/1‑132 (MQ=255)                                                                 tCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGt                                                                  <  2:537446/136‑1 (MQ=255)                                                                 tCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGt                                                                  >  1:537446/1‑136 (MQ=255)                                                                      ttGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCa                                                                                                                        >  1:638882/1‑77 (MQ=255)                                                                      ttGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCa                                                                                                                        <  2:638882/77‑1 (MQ=255)                                                                           agccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAt                                                     <  1:1059380/139‑1 (MQ=255)                                                                               atcatTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCttt                                                             <  2:1449388/127‑1 (MQ=255)                                                                               atcatTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCttt                                                             >  1:1449388/1‑127 (MQ=255)                                                                                      ttCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCAcc                                          >  1:145364/1‑139 (MQ=255)                                                                                       tCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCg                                                                                                                           >  1:1447779/1‑57 (MQ=255)                                                                                       tCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCg                                                                                                                           <  2:1447779/57‑1 (MQ=255)                                                                                       tCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                      <  1:1193127/78‑1 (MQ=255)                                                                                       tCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                      >  2:1193127/1‑78 (MQ=255)                                                                                           cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGt                                                                                                             <  1:361776/67‑1 (MQ=255)                                                                                           cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGt                                                                                                             >  2:361776/1‑67 (MQ=255)                                                                                           cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGc                                                                      <  2:554197/106‑1 (MQ=255)                                                                                           cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGc                                                                      >  1:554197/1‑106 (MQ=255)                                                                                           cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTc                                     >  1:1116476/1‑139 (MQ=255)                                                                                           cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTc                                     >  1:1557854/1‑139 (MQ=255)                                                                                            aGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa                                    >  2:438738/1‑139 (MQ=255)                                                                                              aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCca                                                                                                     >  2:842261/1‑72 (MQ=255)                                                                                              aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCca                                                                                                     >  2:1294696/1‑72 (MQ=255)                                                                                              aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCca                                                                                                     <  1:1294696/72‑1 (MQ=255)                                                                                              aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCca                                                                                                     <  1:842261/72‑1 (MQ=255)                                                                                                tAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGaa                                >  1:1527405/1‑139 (MQ=255)                                                                                                     aCGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCa                                                                                                                  <  1:1343519/52‑1 (MQ=255)                                                                                                     aCGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCa                                                                                                                  >  2:1343519/1‑52 (MQ=255)                                                                                                        gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTc                                                                                                            <  2:621600/55‑1 (MQ=255)                                                                                                        gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTc                                                                                                            >  1:621600/1‑55 (MQ=255)                                                                                                        gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCa                                            >  1:1420203/1‑119 (MQ=255)                                                                                                        gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCa                                            <  2:1420203/119‑1 (MQ=255)                                                                                                        gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGaa                        >  2:1416661/1‑139 (MQ=255)                                                                                                        gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGaa                        >  1:1120214/1‑139 (MQ=255)                                                                                                         cAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGaaa                       <  2:145364/139‑1 (MQ=255)                                                                                                           gATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAAcc                     <  1:787372/139‑1 (MQ=255)                                                                                                                 tATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACg                                                 >  1:1550754/1‑105 (MQ=255)                                                                                                                 tATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACg                                                 <  2:873033/105‑1 (MQ=255)                                                                                                                 tATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACg                                                 <  2:1550754/105‑1 (MQ=255)                                                                                                                 tATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACg                                                 >  1:873033/1‑105 (MQ=255)                                                                                                                     ggTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa                                    >  2:552320/1‑114 (MQ=255)                                                                                                                     ggTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa                                    <  1:552320/114‑1 (MQ=255)                                                                                                                      gTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCa          >  2:560996/1‑139 (MQ=255)                                                                                                                       tAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                           >  1:692570/1‑57 (MQ=255)                                                                                                                       tAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                           <  2:692570/57‑1 (MQ=255)                                                                                                                       tAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGc                                                                           <  1:643599/73‑1 (MQ=255)                                                                                                                       tAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGc                                                                           >  2:643599/1‑73 (MQ=255)                                                                                                                       tAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGt                                                                  <  1:1011016/82‑1 (MQ=255)                                                                                                                       tAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGt                                                                  >  2:1011016/1‑82 (MQ=255)                                                                                                                          ggaggaAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTc                                             <  1:1534217/100‑1 (MQ=255)                                                                                                                          ggaggaAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTc                                             >  2:1534217/1‑100 (MQ=255)                                                                                                                           gaggaAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCgg     <  1:438738/139‑1 (MQ=255)                                                                                                                           gaggaAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCgg     <  1:560996/139‑1 (MQ=255)                                                                                                                              gaAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg  <  1:356451/139‑1 (MQ=255)                                                                                                                                aaTGCAGGGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCttt                                                             <  1:1476713/78‑1 (MQ=255)                                                                                                                                aaTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCttt                                                             >  2:1476713/1‑78 (MQ=255)                                                                                                                                     agagTTATTCGTCACGTTCAATGGTCAATGCCACAcc                                                                                                 >  2:1319271/1‑37 (MQ=255)                                                                                                                                     agagTTATTCGTCACGTTCAATGGTCAATGCCACAcc                                                                                                 <  1:1319271/37‑1 (MQ=255)                                                                                                                                      gagTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCa                                                                    <  2:1317520/65‑1 (MQ=255)                                                                                                                                         |                                                                                                                                AGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAG  >  NC_000913/2984275‑2984538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑