Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,999,269	G→A	A208V (GCT→GTT)	ygeR ←	LysM domain‑containing M23 family putative peptidase, septation lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,999,269	0	G	A	100.0%	240.2 / NA	73	A208V (GCT→GTT)	ygeR	LysM domain‑containing M23 family putative peptidase, septation lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/39);  total (34/39)

CATCAGCATTTTGGCTTGCTGCCCTGAGGCGGCAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAGCCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGTTGCCCACATACACCAC  >  NC_000913/2999134‑2999396                                                                                                                                        |                                                                                                                                cATCAGCATTTTGGCTTGCTGCCCTGAGGCGGCAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCt                                                                                                                              >  2:650716/1‑139 (MQ=255)      gCATTTTGGCTTGCTGCCCTGAGGCGGCAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACg                                                                                                                         >  1:505775/1‑139 (MQ=255)       cATTTTGGCTTGCTGCCCTGAGGCGGCAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGc                                                                                                                        >  1:55567/1‑139 (MQ=255)             ggCTTGCTGCCCTGAGGCGGCAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGcc                                                                                                                  <  2:505775/139‑1 (MQ=255)               cTTGCTGCCCTGAGGCGGCAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCa                                                                                                                >  2:1317823/1‑139 (MQ=255)                 tgctgcCCTGAGGCGGCAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGcc                                                                                                                  <  1:1300141/135‑1 (MQ=255)                 tgctgcCCTGAGGCGGCAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGcc                                                                                                                  >  2:1300141/1‑135 (MQ=255)                   ctgcCCTGAGGCGGCAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCtt                                                                                                                             <  2:809261/122‑1 (MQ=255)                   ctgcCCTGAGGCGGCAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCtt                                                                                                                             >  1:809261/1‑122 (MQ=255)                            ggcggcAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCa                                                                                                   <  1:650716/139‑1 (MQ=255)                            ggcggcAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCa                                                                                                   <  2:1557573/139‑1 (MQ=255)                                gcAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGc                                                                                                    <  2:441247/134‑1 (MQ=255)                                gcAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGc                                                                                                    >  1:441247/1‑134 (MQ=255)                                gcAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCgt                                                                                               >  1:1362694/1‑139 (MQ=255)                                gcAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCgt                                                                                               >  2:557310/1‑139 (MQ=255)                                gcAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCgt                                                                                               >  1:441830/1‑139 (MQ=255)                                       aCGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATG‑CGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTAt                                                                                       <  1:453460/139‑1 (MQ=255)                                             cGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCatta                                                                                                             >  2:1066460/1‑112 (MQ=255)                                             cGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCatta                                                                                                             <  1:1066460/112‑1 (MQ=255)                                              ggATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGt                                                                                 <  2:1401702/139‑1 (MQ=255)                                               gATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCa                                                                                                                <  2:1105661/107‑1 (MQ=255)                                               gATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCa                                                                                                                >  1:1105661/1‑107 (MQ=255)                                               gATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTa                                                                                <  2:1509798/139‑1 (MQ=255)                                                   aaTTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCatta                                                                                                             >  1:257685/1‑106 (MQ=255)                                                   aaTTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCatta                                                                                                             <  2:257685/106‑1 (MQ=255)                                                        ccGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCgtaat                                                                       <  2:55567/139‑1 (MQ=255)                                                          gTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAg                                                                              <  2:744149/130‑1 (MQ=255)                                                          gTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAg                                                                              >  1:744149/1‑130 (MQ=255)                                                          gTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCgtaatgt                                                                     >  2:923810/1‑139 (MQ=255)                                                                  gTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCt                                                                                                                       <  1:590337/81‑1 (MQ=255)                                                                  gTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCt                                                                                                                       >  2:590337/1‑81 (MQ=255)                                                                   tAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTc                                                                                                                            <  1:345191/75‑1 (MQ=255)                                                                   tAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTc                                                                                                                            >  2:345191/1‑75 (MQ=255)                                                                    aaCGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACt                                                           <  1:188295/139‑1 (MQ=255)                                                                        aaTCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTc                                                                                                                            >  1:389684/1‑70 (MQ=255)                                                                        aaTCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTc                                                                                                                            <  2:389684/70‑1 (MQ=255)                                                                         aTCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGtt                                                      <  1:557310/139‑1 (MQ=255)                                                                             ggAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGcc                                                                                                                  >  2:459715/1‑75 (MQ=255)                                                                             ggAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGcc                                                                                                                  <  1:459715/75‑1 (MQ=255)                                                                             ggAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCatta                                                                                                             <  1:774568/80‑1 (MQ=255)                                                                             ggAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCatta                                                                                                             >  2:774568/1‑80 (MQ=255)                                                                             ggAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAAt                                                  >  1:432632/1‑139 (MQ=255)                                                                                       gCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga                                        <  2:1362694/139‑1 (MQ=255)                                                                                             aGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCa                                                                                                                <  2:964892/61‑1 (MQ=255)                                                                                             aGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCa                                                                                                                >  1:964892/1‑61 (MQ=255)                                                                                                     cATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCatta                                                                                                             <  1:1121644/56‑1 (MQ=255)                                                                                                     cATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCatta                                                                                                             >  2:1121644/1‑56 (MQ=255)                                                                                                     cATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCgt                                                                                               <  1:273248/70‑1 (MQ=255)                                                                                                     cATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCgt                                                                                               >  2:273248/1‑70 (MQ=255)                                                                                                         cGTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGc                      <  2:441830/139‑1 (MQ=255)                                                                                                          gTGCTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCt                     >  2:1594658/1‑139 (MQ=255)                                                                                                             cTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGt                  <  1:840080/139‑1 (MQ=255)                                                                                                             cTCCCCATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGt                  <  2:432632/139‑1 (MQ=255)                                                                                                                cccATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGTTGc               <  1:157072/139‑1 (MQ=255)                                                                                                                  cATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTa                                                                                        >  2:340888/1‑64 (MQ=255)                                                                                                                  cATAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTa                                                                                        <  1:340888/64‑1 (MQ=255)                                                                                                                   aTAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCa                                                                                                                >  1:288426/1‑39 (MQ=255)                                                                                                                   aTAGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCa                                                                                                                <  2:288426/39‑1 (MQ=255)                                                                                                                     aGTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCTTGATGAGATTACCGTAGCCACGCAGCTGGTTGCCcaca          <  2:429957/139‑1 (MQ=255)                                                                                                                      gTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCa                                                             >  2:1298369/1‑87 (MQ=255)                                                                                                                      gTGGCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCa                                                             <  1:1298369/87‑1 (MQ=255)                                                                                                                         gCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGtt                                                      <  1:407015/91‑1 (MQ=255)                                                                                                                         gCGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGtt                                                      >  2:407015/1‑91 (MQ=255)                                                                                                                          cGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCgt                                                                                               >  2:573845/1‑49 (MQ=255)                                                                                                                          cGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCgt                                                                                               <  1:573845/49‑1 (MQ=255)                                                                                                                          cGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTa                                                                                        <  1:1288219/56‑1 (MQ=255)                                                                                                                          cGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTa                                                                                        >  1:818979/1‑56 (MQ=255)                                                                                                                          cGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTa                                                                                        <  2:818979/56‑1 (MQ=255)                                                                                                                          cGATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTa                                                                                        >  2:1288219/1‑56 (MQ=255)                                                                                                                           gATTTTTTGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGTTGCCCACATAcacc    >  2:1392609/1‑139 (MQ=255)                                                                                                                             ttttttGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGTTGCCCACATAcaccac  <  1:1392609/139‑1 (MQ=255)                                                                                                                                 ttGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAg                                                                              >  1:290977/1‑59 (MQ=255)                                                                                                                                 ttGCCCAACCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAg                                                                              <  2:290977/59‑1 (MQ=255)                                                                                                                                        |                                                                                                                                CATCAGCATTTTGGCTTGCTGCCCTGAGGCGGCAAGTAACGTAGCGGATCAATTGCCGTTGCACGGTAACGAATCTGGAAATGCAGGCGAACAGATGCCGCATCCGTGCTCCCCATAGTGGCGATTTTTTGCCCAGCCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGTTGCCCACATACACCAC  >  NC_000913/2999134‑2999396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑