Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,068,173	C→T	*212* (TAG→TAA)	argO ←	arginine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,068,173	0	C	T	100.0%	144.1 / NA	45	*212* (TAG→TAA)	argO	arginine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/22);  total (23/22)

CTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTGCCAGCC  >  NC_000913/3068037‑3068303                                                                                                                                         |                                                                                                                                   cTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTa                                                                                                                                  >  1:478940/1‑139 (MQ=255)  tAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTaa                                                                                                                                 <  2:435958/139‑1 (MQ=255)   aaTACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTGGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAAc                                                                                                                                >  1:942541/1‑139 (MQ=255)       cccATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGaa                                                                                                                            >  2:700662/1‑139 (MQ=255)        ccATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAAc                                                                                                                           <  1:700662/139‑1 (MQ=255)          aTTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACaa                                                                                                                         >  1:126861/1‑139 (MQ=255)           ttAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAg                                                                                                                        >  2:544285/1‑139 (MQ=255)             aaTGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGc                                                                                                                      <  2:271920/139‑1 (MQ=255)              aTGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACTTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCt                                                                                                                     >  1:1496918/1‑139 (MQ=255)                 ccgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTtgt                                                                                                                  >  2:288602/1‑139 (MQ=255)                             tAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATa                                                                                                      <  1:288602/139‑1 (MQ=255)                                          cACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAg                                                                                                           <  2:222163/121‑1 (MQ=255)                                          cACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAg                                                                                                           >  1:222163/1‑121 (MQ=255)                                              ttAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTg                                                                                     <  2:942541/139‑1 (MQ=255)                                                     ctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCa                                                                              <  2:1496918/139‑1 (MQ=255)                                                               aTTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACcaca                                                                    <  2:1375404/139‑1 (MQ=255)                                                                ttGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACAt                                                                   >  2:1257961/1‑139 (MQ=255)                                                                       gCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAAc                                                                                                                                <  1:1379737/71‑1 (MQ=255)                                                                       gCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAAc                                                                                                                                >  2:1379737/1‑71 (MQ=255)                                                                        cccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                                                <  2:1574747/86‑1 (MQ=255)                                                                        cccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                                                >  1:1574747/1‑86 (MQ=255)                                                                                         ctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGaa                                                                                                                            >  1:1513175/1‑57 (MQ=255)                                                                                         ctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGaa                                                                                                                            <  2:1513175/57‑1 (MQ=255)                                                                                               gCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                                    <  1:1257961/139‑1 (MQ=255)                                                                                                   gCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTaa                                                                                                                                 >  1:1598556/1‑42 (MQ=255)                                                                                                   gCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTaa                                                                                                                                 <  2:1598556/42‑1 (MQ=255)                                                                                                   gCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgtg                                >  1:366072/1‑139 (MQ=255)                                                                                                         cgcCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGaa                                                                                                                            <  2:498917/41‑1 (MQ=255)                                                                                                         cgcCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGaa                                                                                                                            >  1:498917/1‑41 (MQ=255)                                                                                                             tctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAaca                                                               >  2:1233612/1‑98 (MQ=255)                                                                                                             tctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAaca                                                               <  1:1233612/98‑1 (MQ=255)                                                                                                             tctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGt                      >  1:695082/1‑139 (MQ=255)                                                                                                              ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCaa                                                                                  <  1:825460/78‑1 (MQ=255)                                                                                                              ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCaa                                                                                  >  2:825460/1‑78 (MQ=255)                                                                                                              ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTg                     >  2:1417542/1‑139 (MQ=255)                                                                                                                      tttttCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCa                                                  >  1:1138652/1‑102 (MQ=255)                                                                                                                      tttttCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCa                                                  <  2:1138652/102‑1 (MQ=255)                                                                                                                         ttCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGcc                                                                                          >  2:282256/1‑59 (MQ=255)                                                                                                                         ttCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGcc                                                                                          <  1:282256/59‑1 (MQ=255)                                                                                                                           ccATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                           >  1:1464389/1‑72 (MQ=255)                                                                                                                           ccATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                           <  2:1464389/72‑1 (MQ=255)                                                                                                                           ccATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                                          >  2:175137/1‑89 (MQ=255)                                                                                                                           ccATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                                          <  1:175137/89‑1 (MQ=255)                                                                                                                            cATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTgc       <  1:1417542/139‑1 (MQ=255)                                                                                                                                 gATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTgccagcc  <  1:544285/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   CTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTGCCAGCC  >  NC_000913/3068037‑3068303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑