Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,134,823 G→A *231* (TAG→TAA yghT → putative ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,134,8230GA100.0% 146.5 / NA 46*231* (TAG→TAAyghTputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/22);  total (24/22)

TGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATGC  >  NC_000913/3134721‑3134957
                                                                                                      |                                                                                                                                      
tgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGcct                                                                                                   >  2:369815/1‑137 (MQ=255)
       cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                            <  2:506658/139‑1 (MQ=255)
        tGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGa                                                                                           <  1:181513/139‑1 (MQ=255)
          gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                         >  1:1004979/1‑139 (MQ=255)
                    aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATc                                                                                                                                      <  1:64905/84‑1 (MQ=255)
                       ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                            <  1:225926/139‑1 (MQ=255)
                             aGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGc                                                                      >  2:952491/1‑139 (MQ=255)
                                               tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                       <  1:49940/120‑1 (MQ=255)
                                               tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                       >  2:49940/1‑120 (MQ=255)
                                                ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATc                                                                                                                                      >  2:245414/1‑56 (MQ=255)
                                                ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                       <  2:531627/119‑1 (MQ=255)
                                                ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                       >  1:531627/1‑119 (MQ=255)
                                                     aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                                     <  2:898829/68‑1 (MQ=255)
                                                     aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                                     <  1:1310730/68‑1 (MQ=255)
                                                     aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                                     >  1:898829/1‑68 (MQ=255)
                                                     aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                                     >  2:1310730/1‑68 (MQ=255)
                                                     aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                              >  2:16671/1‑139 (MQ=255)
                                                      tGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                                                      >  1:1548531/1‑82 (MQ=255)
                                                      tGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                                                      <  2:1548531/82‑1 (MQ=255)
                                                      tGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaaga                                             >  2:12460/1‑139 (MQ=255)
                                                                gCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                                                      <  2:692378/72‑1 (MQ=255)
                                                                gCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                                                      >  1:692378/1‑72 (MQ=255)
                                                                gCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                                   >  2:1192809/1‑139 (MQ=255)
                                                                   aTTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                                     <  1:1159792/54‑1 (MQ=255)
                                                                   aTTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                                     >  2:1159792/1‑54 (MQ=255)
                                                                                 gACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTTAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATAcc                  <  2:1004979/139‑1 (MQ=255)
                                                                                    cgcgcgGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                                                      >  2:234476/1‑52 (MQ=255)
                                                                                    cgcgcgGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                                                      <  1:234476/52‑1 (MQ=255)
                                                                                      gcgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCa                <  2:1335242/132‑1 (MQ=255)
                                                                                      gcgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCa                >  1:1335242/6‑137 (MQ=255)
                                                                                      gcgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACg              <  1:12460/134‑1 (MQ=255)
                                                                                       cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGc                                                                                                     >  2:884637/5‑51 (MQ=255)
                                                                                       cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGc                                                                                                     <  1:884637/47‑1 (MQ=255)
                                                                                       cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                            <  2:716148/56‑1 (MQ=255)
                                                                                       cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                            >  1:716148/5‑60 (MQ=255)
                                                                                       cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                       <  1:439653/77‑1 (MQ=255)
                                                                                       cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                       <  2:917859/77‑1 (MQ=255)
                                                                                       cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                       >  2:439653/5‑81 (MQ=255)
                                                                                       cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                       >  1:917859/5‑81 (MQ=255)
                                                                                       cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                             >  2:942472/5‑123 (MQ=255)
                                                                                       cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc             >  1:1316739/5‑139 (MQ=255)
                                                                                       cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc             >  2:984295/5‑139 (MQ=255)
                                                                                       cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTCCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                             <  1:942472/119‑1 (MQ=255)
                                                                                                tCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGAt    <  1:369815/139‑1 (MQ=255)
                                                                                                tCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGAt    <  2:1316739/139‑1 (MQ=255)
                                                                                                  aCTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATGc  >  2:1335479/1‑139 (MQ=255)
                                                                                                      |                                                                                                                                      
TGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATGC  >  NC_000913/3134721‑3134957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: