Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	316,453	G→A	*289* (TAG→TAA)	insF1 →	IS3 transposase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	316,453	0	G	A	100.0%	131.4 / NA	41	*289* (TAG→TAA)	insF1	IS3 transposase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/21);  total (20/21)

TGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAGCATATTCACATACCGTACCAGCAAAAGTTTCAACCGTGAGCAAATCGAC  >  NC_000913/316356‑316572                                                                                                  |                                                                                                                        tgtTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCg                                                                                <  2:155196/139‑1 (MQ=255)        ttATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGtt                                                                         >  2:1318406/1‑139 (MQ=255)           tatCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTc                                                                      >  2:510690/1‑139 (MQ=255)             tCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCggg                                                                              <  2:1115003/129‑1 (MQ=255)             tCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCa                                                                    >  1:514975/1‑139 (MQ=255)             tCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCGCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCggg                                                                              >  1:1115003/1‑129 (MQ=255)                 aTGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTc                                                                            >  2:1584030/1‑127 (MQ=255)                 aTGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTc                                                                            <  1:1584030/127‑1 (MQ=255)                  tgtgATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCtt                                                               >  2:1113095/1‑139 (MQ=255)                  tgtgATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCtt                                                               >  2:68374/1‑139 (MQ=255)                  tgtgATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCtt                                                               >  2:1388884/1‑139 (MQ=255)                   ccgccgccAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCttt                                                              >  1:408862/8‑139 (MQ=255)                      aTTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTgg                                                           <  1:375824/139‑1 (MQ=255)                        tACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCa                                                         <  2:514975/139‑1 (MQ=255)                          cAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATg                                                       <  1:1388884/139‑1 (MQ=255)                            aTCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTc                                                     <  1:1318406/139‑1 (MQ=255)                             tCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTTGGATCATAAAGTCCTCGGGTCGTTGTc                                                                      <  1:960707/121‑1 (MQ=255)                             tCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGATAGGATCATAAAGTCCTCGGGTCGTTGTc                                                                      >  2:960707/1‑121 (MQ=255)                                gTGGCGGCGGCACAGTTGGTGTGGTGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTc                                                                            >  2:1553982/1‑112 (MQ=255)                                gTGGCGGCGGCACAGTTGGTGTGGTGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTc                                                                            <  1:1553982/112‑1 (MQ=255)                                gTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAGCa                                                 <  1:510690/139‑1 (MQ=255)                                gTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAGCa                                                 <  1:1113095/139‑1 (MQ=255)                                 tGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCa                                                                    >  1:353348/1‑119 (MQ=255)                                 tGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCa                                                                    <  2:353348/119‑1 (MQ=255)                                         gcACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAGCa                                                 >  1:255391/1‑130 (MQ=255)                                         gcACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAGCa                                                 <  2:255391/130‑1 (MQ=255)                                                    gtgGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGAGTCGTTGTCCa                                                                    <  1:1557514/100‑1 (MQ=255)                                                    gtgGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGAGTCGTTGTCCa                                                                    >  2:1557514/1‑100 (MQ=255)                                                       gcggcCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAg                                                   <  1:296414/114‑1 (MQ=255)                                                       gcggcCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAg                                                   >  2:296414/1‑114 (MQ=255)                                                         ggcCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAGCATATTCACATACCGTACCAGCAAAAg                        >  2:419069/1‑139 (MQ=255)                                                            cTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAGCATATTCACATACCGTACCAGCAAAAGttt                     <  2:1461511/139‑1 (MQ=255)                                                            cTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAGCATATTCACATACCGTACCAGCAAAAGttt                     <  1:1250035/139‑1 (MQ=255)                                                            cTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAGCATATTCACATACCGTACCAGCAAAAGttt                     >  2:1334415/1‑139 (MQ=255)                                                                           tttGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTc                                                                            >  1:88919/1‑69 (MQ=255)                                                                           tttGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTc                                                                            <  2:88919/69‑1 (MQ=255)                                                                           tttGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATg                                                       <  1:1474072/90‑1 (MQ=255)                                                                           tttGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATg                                                       >  2:1474072/1‑90 (MQ=255)                                                                               aaaaCAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAGCATATTCACATACCGTACCAGCAAAAGTTTCAACCGTGAGCAAATCGAc  <  1:650965/139‑1 (MQ=255)                                                                                              cTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAGCa                                                 <  1:159137/77‑1 (MQ=255)                                                                                              cTTAAGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAGCa                                                 >  2:159137/1‑77 (MQ=255)                                                                                                  |                                                                                                                        TGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCATAAAGTCCTCGGGTCGTTGTCCACTCTTTTGGCATGTCAGCATATTCACATACCGTACCAGCAAAAGTTTCAACCGTGAGCAAATCGAC  >  NC_000913/316356‑316572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑