Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,269,602	G→A	*396* (TAG→TAA)	yhaC →	pentapetide repeats‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,269,602	0	G	A	100.0%	77.1 / NA	25	*396* (TAG→TAA)	yhaC	pentapetide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/13);  total (12/13)

AGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACG  >  NC_000913/3269469‑3269736                                                                                                                                      |                                                                                                                                       agaAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTta                                                                                                                                   >  1:1286498/1‑139 (MQ=255)  gaAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTtaa                                                                                                                                  <  2:576140/139‑1 (MQ=255)            aaTTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAAC‑CATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTAc                                                                                                                       <  2:1317607/139‑1 (MQ=255)              ttCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                      <  1:1047926/139‑1 (MQ=255)                    ggACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCt                                                                                                                <  2:1296068/139‑1 (MQ=255)                      aCAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCtat                                                                                                              <  1:667354/139‑1 (MQ=255)                              ttcgAGGTCGGGGAATATAACACATATGATGCAAATAAATCAGAGAAGCAAAATTTTACCAGAGAGGACTTTACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATa                                                                                                      <  1:1559053/136‑1 (MQ=255)                                                  cacaTATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCt                                                                                  >  2:1138524/1‑139 (MQ=255)                                                    caTATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTACTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTa                                                                                <  1:1138524/139‑1 (MQ=255)                                                                ataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACaaa                                                                                                                    <  2:292919/91‑1 (MQ=255)                                                                ataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACaaa                                                                                                                    >  1:292919/1‑91 (MQ=255)                                                                       agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                      >  2:967254/1‑82 (MQ=255)                                                                       agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                      <  1:967254/82‑1 (MQ=255)                                                                       agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCaa                                                             >  1:1387122/1‑139 (MQ=255)                                                                            aGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACt                                                        >  2:497724/1‑139 (MQ=255)                                                                                           gagagGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGttt                                         >  1:814838/1‑139 (MQ=255)                                                                                                      aCGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                              >  2:1438589/1‑91 (MQ=255)                                                                                                      aCGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                              <  1:1438589/91‑1 (MQ=255)                                                                                                       cGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGc                             <  1:497724/139‑1 (MQ=255)                                                                                                          aTGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAc                          >  2:1166624/1‑139 (MQ=255)                                                                                                             aTTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTCCAAAGTCTATTATTAATACCGTAGGTATTTATTCATATTCCCCTGTGCACTCAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACAGc                       >  1:507943/1‑139 (MQ=255)                                                                                                                             tGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCCCAGACGCGGGTTTGAAACTGATGGCTACCGCAAAAATGCCTGATgcg       <  2:746808/139‑1 (MQ=255)                                                                                                                               cAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACt                                   >  2:1347751/1‑109 (MQ=255)                                                                                                                               cAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACt                                   <  1:1347751/109‑1 (MQ=255)                                                                                                                                  tataAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACg  >  1:68892/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                       AGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACG  >  NC_000913/3269469‑3269736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑