Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,277,867	C→T	V267I (GTC→ATC)	agaR ←	transcriptional repressor of the aga regulon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,867	0	C	T	100.0%	310.3 / NA	96	V267I (GTC→ATC)	agaR	transcriptional repressor of the aga regulon
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/51);  total (45/51)

AAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGA  >  NC_000913/3277730‑3277999                                                                                                                                          |                                                                                                                                     aaaaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGA‑‑‑AGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGa                                                                                                                                  <  1:780319/139‑1 (MQ=255)   aaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAg                                                                                                                                   >  1:1203144/1‑139 (MQ=255)            aaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTc                                                                                                                          <  1:665411/139‑1 (MQ=255)            aaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTc                                                                                                                          <  2:441311/139‑1 (MQ=255)               agGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAAc                                                                                                                       >  1:889084/1‑139 (MQ=255)                     tcctccTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGc                                                                                                                 <  1:1392440/139‑1 (MQ=255)                         cctGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGaa                                                                                                                                 >  1:688949/1‑119 (MQ=255)                         cctGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGaa                                                                                                                                 <  2:688949/119‑1 (MQ=255)                           tGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCg                                                                                                           >  2:305859/1‑139 (MQ=255)                                aCTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTc                                                                                                      >  2:230229/1‑139 (MQ=255)                                aCTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTc                                                                                                      >  1:317512/1‑139 (MQ=255)                                aCTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTc                                                                                                      >  2:1094713/1‑139 (MQ=255)                                  tGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCt                                                                                                    >  2:812199/1‑139 (MQ=255)                                    ggAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTc                                                                                                  >  1:785523/1‑139 (MQ=255)                                        aCCCTCTCCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                              <  1:1094713/139‑1 (MQ=255)                                        aCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                              <  2:889084/139‑1 (MQ=255)                                               cccGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCg                                                                                       <  1:444366/139‑1 (MQ=255)                                                   agaAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAgg                                                                                   >  2:840600/1‑139 (MQ=255)                                                    gaAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGa                                                                                  <  2:785523/139‑1 (MQ=255)                                                        cAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGc                                                                              >  2:821555/1‑139 (MQ=255)                                                         agaagaAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc                                                                             <  2:1458745/139‑1 (MQ=255)                                                         agaagaAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc                                                                             <  1:483725/139‑1 (MQ=255)                                                          gaagaaACCCATTGATGGTGTTCCCAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTAACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCt                                                                            >  2:1176561/1‑139 (MQ=255)                                                          gaagaaACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCt                                                                            >  1:569470/1‑139 (MQ=255)                                                              aaaCCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                        <  2:1509362/139‑1 (MQ=255)                                                              aaaCCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                        <  2:275488/139‑1 (MQ=255)                                                                     ttGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAAt                                                                 >  1:1531577/1‑139 (MQ=255)                                                                        aTGGTATTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGCATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                              <  2:1000361/139‑1 (MQ=255)                                                                           gtgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCAt                                                           >  1:560628/1‑139 (MQ=255)                                                                            tgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc                                                                             <  2:851882/120‑1 (MQ=255)                                                                            tgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc                                                                             >  1:851882/1‑120 (MQ=255)                                                                            tgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                        <  2:225778/125‑1 (MQ=255)                                                                            tgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                        >  1:225778/1‑125 (MQ=255)                                                                            tgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATg                                                          <  2:1256957/139‑1 (MQ=255)                                                                             gtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                        <  2:1587299/124‑1 (MQ=255)                                                                             gtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                        >  1:1587299/1‑124 (MQ=255)                                                                              ttCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                        <  1:348837/91‑1 (MQ=255)                                                                              ttCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                        >  2:348837/1‑91 (MQ=255)                                                                                cacaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGa                                                      >  2:688110/1‑139 (MQ=255)                                                                                cacaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGa                                                      >  2:884124/1‑139 (MQ=255)                                                                                 acaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGAt                                                     <  1:230229/139‑1 (MQ=255)                                                                                 acaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGAt                                                     <  1:884124/139‑1 (MQ=255)                                                                                    aTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg                                                  <  2:633204/139‑1 (MQ=255)                                                                                     tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAAt                                                                                                                                <  1:465947/60‑1 (MQ=255)                                                                                     tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAAt                                                                                                                                >  2:465947/1‑60 (MQ=255)                                                                                     tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCt                                                                                            <  2:346138/96‑1 (MQ=255)                                                                                     tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCt                                                                                            >  1:346138/1‑96 (MQ=255)                                                                                      gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGtt                                                <  1:840600/139‑1 (MQ=255)                                                                                      gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGtt                                                <  1:688110/139‑1 (MQ=255)                                                                                      gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGtt                                                <  2:1203144/139‑1 (MQ=255)                                                                                        ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTc                                                                                                  <  2:522266/87‑1 (MQ=255)                                                                                        ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTc                                                                                                  >  1:522266/1‑87 (MQ=255)                                                                                        ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc                                                                             >  1:1512790/1‑108 (MQ=255)                                                                                        ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc                                                                             <  2:1512790/108‑1 (MQ=255)                                                                                          gccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGt                                            <  1:821555/139‑1 (MQ=255)                                                                                             gAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                        >  1:606626/1‑76 (MQ=255)                                                                                             gAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                        <  2:606626/76‑1 (MQ=255)                                                                                             gAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATc                                         >  1:630664/1‑139 (MQ=255)                                                                                               aCGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                              <  2:934404/84‑1 (MQ=255)                                                                                               aCGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCa                                                            >  2:781312/1‑118 (MQ=255)                                                                                               aCGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCa                                                            <  1:781312/118‑1 (MQ=255)                                                                                               aCGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCaa                                       <  1:812199/139‑1 (MQ=255)                                                                                               aCGCATTCGTGATGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                              >  1:934404/1‑84 (MQ=255)                                                                                                      cGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCGGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCCTCATCAACAATGAt                                                              <  2:1325521/109‑1 (MQ=255)                                                                                                      cGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCCTCATCAACAATGAt                                                              >  1:1325521/1‑109 (MQ=255)                                                                                                        tgtTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTAt                              <  2:317512/139‑1 (MQ=255)                                                                                                          ttGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGt                            >  1:881141/1‑139 (MQ=255)                                                                                                             aCCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAAccc                                                                                                                     >  2:1525028/1‑47 (MQ=255)                                                                                                             aCCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAAccc                                                                                                                     <  1:1525028/47‑1 (MQ=255)                                                                                                               cAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACt                       >  2:163343/1‑139 (MQ=255)                                                                                                                 gCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCttt                                                                                                             <  1:1602829/51‑1 (MQ=255)                                                                                                                 gCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCttt                                                                                                             >  2:1602829/1‑51 (MQ=255)                                                                                                                 gCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                              >  1:1436596/1‑98 (MQ=255)                                                                                                                 gCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                              <  2:1436596/98‑1 (MQ=255)                                                                                                                 gCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTgg                     <  2:273734/139‑1 (MQ=255)                                                                                                                  cGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCa                                        >  1:1588915/1‑119 (MQ=255)                                                                                                                  cGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCa                                        <  2:1588915/119‑1 (MQ=255)                                                                                                                   gggCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGaa                   <  2:560628/139‑1 (MQ=255)                                                                                                                       aTCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGt                                            <  2:1152033/110‑1 (MQ=255)                                                                                                                       aTCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGt                                            >  1:1152033/1‑110 (MQ=255)                                                                                                                       aTCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGa                <  2:479839/138‑1 (MQ=255)                                                                                                                       aTCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGa                >  1:479839/1‑138 (MQ=255)                                                                                                                       aTCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGAt               >  1:422486/1‑139 (MQ=255)                                                                                                                          cTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGa            <  2:881141/139‑1 (MQ=255)                                                                                                                           tttCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGaa           <  1:163343/139‑1 (MQ=255)                                                                                                                            ttCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCtt                                                                                                   <  1:862685/50‑1 (MQ=255)                                                                                                                            ttCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCtt                                                                                                   >  2:862685/1‑50 (MQ=255)                                                                                                                            ttCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGtt                                                <  1:1614680/101‑1 (MQ=255)                                                                                                                            ttCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGtt                                                >  2:1614680/1‑101 (MQ=255)                                                                                                                              ctcACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACtt        <  2:569470/139‑1 (MQ=255)                                                                                                                                   tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTgg   >  2:1279380/1‑139 (MQ=255)                                                                                                                                    ccccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGAt                                   >  1:1540427/1‑106 (MQ=255)                                                                                                                                    ccccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGAt                                   <  2:1540427/106‑1 (MQ=255)                                                                                                                                    ccccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGa  >  1:555177/1‑139 (MQ=255)                                                                                                                                     cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg                                                  <  1:247647/90‑1 (MQ=255)                                                                                                                                     cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg                                                  >  2:247647/1‑90 (MQ=255)                                                                                                                                          |                                                                                                                                     AAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGA  >  NC_000913/3277730‑3277999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑