Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,292,538 G→A A266A (GCC→GCT)  rsmI ← 16S rRNA C1402 2'‑O‑ribose methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,292,5380GA100.0% 226.2 / NA 70A266A (GCC→GCT) rsmI16S rRNA C1402 2'‑O‑ribose methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/33);  total (37/33)

CCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGACCTTCGACAATCAGCACCATTTCGCCTTTGCGA  >  NC_000913/3292410‑3292667
                                                                                                                                |                                                                                                                                 
ccaTTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGcgccg                                                                                                                         <  2:755483/139‑1 (MQ=255)
   ttACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGcgccgccg                                                                                                                      <  2:625523/139‑1 (MQ=255)
           aTCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTc                                                                                                              >  1:876754/1‑139 (MQ=255)
           aTCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTc                                                                                                              >  1:1452051/1‑139 (MQ=255)
             cGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAg                                                                                                            >  2:1003247/1‑139 (MQ=255)
             cGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAg                                                                                                            >  1:514069/1‑139 (MQ=255)
              ctGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTATGCAGCCAGCGCCGCCGCTTTTTTCAGc                                                                                                           >  1:1005064/2‑139 (MQ=255)
                     aaaaGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGtt                                                                                                    <  2:514069/139‑1 (MQ=255)
                                ttGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTagc                                                                                         >  2:1604170/1‑139 (MQ=255)
                                         gATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGtt                                                                                                    >  1:867210/1‑119 (MQ=255)
                                         gATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGtt                                                                                                    <  2:867210/119‑1 (MQ=255)
                                              acgcaacgcTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTgcg                                                                           <  1:1604170/139‑1 (MQ=255)
                                                    cgcTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGc                                                                     >  1:1367696/1‑139 (MQ=255)
                                                        tCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATcg                                                                 <  2:1005064/139‑1 (MQ=255)
                                                           gCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATcggcg                                                              <  1:816933/139‑1 (MQ=255)
                                                               tGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTa                                                          >  1:1228913/1‑139 (MQ=255)
                                                                             ctCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGcgccg                                                                                                                         >  2:484306/1‑62 (MQ=255)
                                                                             ctCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGcgccg                                                                                                                         <  1:484306/62‑1 (MQ=255)
                                                                             ctCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCGGCGGCAGTTCTGCCTGTagcag                                                                                       >  1:1414053/1‑96 (MQ=255)
                                                                             ctCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCGGCGGCAGTTCTGCCTGTagcag                                                                                       <  2:1414053/96‑1 (MQ=255)
                                                                               ccAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGc                                          >  2:1381798/1‑139 (MQ=255)
                                                                                     cATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATg                                    <  2:1452051/139‑1 (MQ=255)
                                                                                       tACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCtgtg                                           <  1:1097374/130‑1 (MQ=255)
                                                                                       tACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCACCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGAc                                  <  2:876754/139‑1 (MQ=255)
                                                                                       tACATATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCCTCCtgtg                                           >  2:1097374/1‑130 (MQ=255)
                                                                                                aGCGCATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGACCTTCGACaa                         <  1:1381798/139‑1 (MQ=255)
                                                                                                 gcgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCt                                                                                              <  2:1008810/69‑1 (MQ=255)
                                                                                                 gcgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCt                                                                                              >  1:1008810/1‑69 (MQ=255)
                                                                                                  ctcATTTTTCTTCACGCCGTGAATTTTTGCAGCCAGCGCCGCCGCTTTTTTCAGCgg                                                                                                         >  1:967658/3‑57 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAgcg                                                                                                                            <  1:1214251/38‑1 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAgcg                                                                                                                            >  2:1214251/1‑38 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCTGCTTTTTTCAGCg                                                                                                          <  1:832118/56‑1 (MQ=39)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTc                                                                                                              <  1:1204175/52‑1 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTc                                                                                                              >  2:1204175/1‑52 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCTGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTACTTCCTGTGCTTTATGACCTTCGACAATc                       >  2:1091020/1‑139 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCgg                                                                                                         <  2:967658/57‑1 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCg                                                                                                          >  2:832118/1‑56 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCt                                                                                              >  2:969729/1‑68 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCt                                                                                              <  1:969729/68‑1 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGt                                                                                            <  1:180335/70‑1 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGt                                                                                            >  2:180335/1‑70 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTag                                                                                          >  2:990702/1‑72 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTag                                                                                          >  1:1275426/1‑72 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTag                                                                                          <  2:1275426/72‑1 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTag                                                                                          <  1:990702/72‑1 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAg                                                                                 >  1:1472874/1‑81 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAg                                                                                 <  2:1472874/81‑1 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATcgg                                                                >  1:1296525/1‑98 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATcgg                                                                <  2:1296525/98‑1 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCgggga                                                          >  1:1385457/1‑102 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTa                                                          <  2:1385457/104‑1 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCtg                                             >  1:1577616/1‑117 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCtg                                             <  2:1577616/117‑1 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGAc                                  >  1:798254/1‑128 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGAc                                  <  2:798254/128‑1 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGACCTTCGACAATc                       >  1:852104/1‑139 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGACCTTCGACAATc                       >  2:769776/1‑139 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGACCTTCGACAATc                       >  1:1526105/1‑139 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGACCTTCGACAATc                       >  2:1369300/1‑139 (MQ=255)
                                                                                                  cgcATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCACCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGACCTTCGACAATc                       >  2:66162/1‑139 (MQ=255)
                                                                                                    cATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGtt                                                                                                    >  2:1262399/1‑60 (MQ=255)
                                                                                                    cATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGtt                                                                                                    <  1:1262399/60‑1 (MQ=255)
                                                                                                    cATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCtg                                             <  2:159355/115‑1 (MQ=255)
                                                                                                    cATTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCtg                                             >  1:159355/1‑115 (MQ=255)
                                                                                                     aTTTTTCTTCACGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGACCTTCGACAATCAGc                    <  2:1367696/139‑1 (MQ=255)
                                                                                                               aCGCCGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGACCTTCGACAATCAGCACCATTTCGc          <  1:769776/139‑1 (MQ=255)
                                                                                                                   cGTGAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGACCTTCGACAATCAGCACCATTTCGCCttt      >  2:541642/1‑139 (MQ=255)
                                                                                                                      gAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGtt                                                                                                    <  1:1331065/42‑1 (MQ=255)
                                                                                                                      gAATTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGtt                                                                                                    >  2:1331065/1‑42 (MQ=255)
                                                                                                                       aaTTTCTGCAGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGACCTTCGACAATCAGCACCATTTCGCCTTTGCGa  <  1:541642/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                 
CCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCAGCGGCAGTTCTGCCTGTAGCAGCGCCAGCGTGCGCAGGGCATCGGCGGGTAAGTCTTCTTCCTGTGCTTTATGACCTTCGACAATCAGCACCATTTCGCCTTTGCGA  >  NC_000913/3292410‑3292667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑