Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,385,857	C→T	*91* (TAG→TAA)	yhcO ←	putative barnase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,385,857	0	C	T	100.0%	181.4 / NA	57	*91* (TAG→TAA)	yhcO	putative barnase inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/29);  total (28/29)

AAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAA  >  NC_000913/3385724‑3385972                                                                                                                                       |                                                                                                                    aaaGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAAtt                                                                                                                >  1:420917/1‑138 (MQ=255) aaaGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATg                                                                                                                >  1:943736/1‑139 (MQ=255)      cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACg                                                                                                             <  2:1510307/137‑1 (MQ=255)      cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACg                                                                                                             >  1:1510307/1‑137 (MQ=255)                     gATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAAt                                                                                            <  2:1158971/139‑1 (MQ=255)                        tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGcc                                                                                         >  2:1357899/1‑139 (MQ=255)                           tGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCtt                                                                                      >  1:1365109/1‑139 (MQ=255)                                 tCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                <  1:46648/139‑1 (MQ=255)                                    tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCtt                                                                             >  1:474936/1‑139 (MQ=255)                                          tGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                       >  2:735533/1‑139 (MQ=255)                                             cacCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCt                                                                    <  1:119368/139‑1 (MQ=255)                                               ccTGGCACGCTACGGCTGAACTGTAC‑AATAAACCCTCATCGGCCTTTCCGACGAAATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGAAGAACATTTAAACGCAAATGCCCCTCCAGCTTTTCCTCTGCCtcat                                                                 >  2:561192/1‑139 (MQ=255)                                                 tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCtt                                                                                      >  2:529620/1‑117 (MQ=255)                                                 tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCtt                                                                                      <  1:529620/117‑1 (MQ=255)                                                 tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatc                                                                >  2:830655/1‑139 (MQ=255)                                                    cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                             <  1:230443/139‑1 (MQ=255)                                                      cGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACa                                                           <  1:1357899/139‑1 (MQ=255)                                                       gCTACGGCTGAACTGTACAAATAACCCCTCACCGTTTGGCCCGCGGATATTGCCCCCGGTTCGGGGGT‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                          <  2:420917/139‑1 (MQ=255)                                                       gCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                          <  2:943736/139‑1 (MQ=255)                                                       gCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                          <  1:735533/139‑1 (MQ=255)                                                       gCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                          <  2:1365109/139‑1 (MQ=255)                                                                      tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATg                                                                                                                <  2:87388/70‑1 (MQ=255)                                                                      tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATg                                                                                                                >  1:87388/1‑70 (MQ=255)                                                                           aTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCt                                                                                       >  2:1571793/1‑90 (MQ=255)                                                                           aTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCt                                                                                       <  1:1571793/90‑1 (MQ=255)                                                                              aaCCCTCATCGTCTTGTCCGACGATATTGCGCCCAGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAGCCGCAATATTAACGCGCCAAAACGGc                                   <  1:1188666/139‑1 (MQ=255)                                                                                cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAAt                                                                                                                 <  1:1236776/59‑1 (MQ=255)                                                                                cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAAt                                                                                                                 >  2:1236776/1‑59 (MQ=255)                                                                                cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                    <  1:75206/88‑1 (MQ=255)                                                                                cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                    >  2:75206/1‑88 (MQ=255)                                                                                cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                >  1:368233/1‑92 (MQ=255)                                                                                cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                <  2:368233/92‑1 (MQ=255)                                                                                   tCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAcgc                              >  2:661226/1‑139 (MQ=255)                                                                                     aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTc                                                                                     >  1:159455/1‑82 (MQ=255)                                                                                     aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTc                                                                                     <  2:159455/82‑1 (MQ=255)                                                                                     aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTaa                                                 <  1:423253/118‑1 (MQ=255)                                                                                     aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTaa                                                 >  2:423253/1‑118 (MQ=255)                                                                                     aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGt                            >  2:1252004/1‑139 (MQ=255)                                                                                             gTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                             >  1:1260055/1‑98 (MQ=255)                                                                                             gTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                             <  2:1260055/98‑1 (MQ=255)                                                                                             gTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAc                                                            >  2:357085/1‑99 (MQ=255)                                                                                             gTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAc                                                            <  1:357085/99‑1 (MQ=255)                                                                                               ccGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTccc                  <  2:474936/139‑1 (MQ=255)                                                                                                   cgaTATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                          <  1:1172492/95‑1 (MQ=255)                                                                                                   cgaTATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                          >  2:1172492/1‑95 (MQ=255)                                                                                                       atTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAAc          >  2:1180794/1‑139 (MQ=255)                                                                                                        ttGCCCCCGGTTCGGGGGCTTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGttt                          <  2:1397114/123‑1 (MQ=255)                                                                                                        ttGCCCCCGGTTCGGGGGCTTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGttt                          >  1:1397114/1‑123 (MQ=255)                                                                                                             cccGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTc    >  2:623461/1‑139 (MQ=255)                                                                                                               cGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCaa  <  1:470485/139‑1 (MQ=255)                                                                                                                ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTcc                                                                           >  2:231761/1‑65 (MQ=255)                                                                                                                ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTcc                                                                           <  1:231761/65‑1 (MQ=255)                                                                                                                ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGc                                                         >  2:1295708/1‑83 (MQ=255)                                                                                                                ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGc                                                         <  1:1295708/83‑1 (MQ=255)                                                                                                                             tttttttGCGTTAATGACGAACATTAAACCGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                               >  1:826656/1‑65 (MQ=255)                                                                                                                             tttttttGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                               <  2:826656/65‑1 (MQ=255)                                                                                                                             tttttttGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAc                                                            <  2:731288/68‑1 (MQ=255)                                                                                                                             tttttttGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAc                                                            >  1:731288/1‑68 (MQ=255)                                                                                                                                       |                                                                                                                    AAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAA  >  NC_000913/3385724‑3385972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑