Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,478,592	C→T	*67* (TAG→TAA)	yheV ←	DUF2387 family putative metal‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,478,592	0	C	T	100.0%	196.0 / NA	60	*67* (TAG→TAA)	yheV	DUF2387 family putative metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/31);  total (29/31)

ATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACAT  >  NC_000913/3478459‑3478723                                                                                                                                      |                                                                                                                                    aTAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTc                                                                                                                                >  2:1539263/1‑139 (MQ=255)    ggCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCgg                                                                                                                             >  1:1399041/1‑139 (MQ=255)               ccaccaGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaa                                                                                                                        >  2:170283/1‑133 (MQ=255)               ccaccaGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaa                                                                                                                        <  1:170283/133‑1 (MQ=255)               ccaccaGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATcc                                                                                                                  >  2:743135/1‑139 (MQ=255)               ccaccaGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTATGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCAGGATGAAAAATcc                                                                                                                  >  1:1016399/1‑139 (MQ=255)                 accaGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCg                                                                                                                <  1:1539263/139‑1 (MQ=255)                    aGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc                                                                                                             <  2:1279506/139‑1 (MQ=255)                                 cTTTCATGATATCTCCTTAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTGACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCtt                                                                                                >  1:922353/1‑139 (MQ=255)                                         atatCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACg                                                                                        <  2:922353/139‑1 (MQ=255)                                           atCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTg                                                                                      >  1:1400201/1‑139 (MQ=255)                                               ccTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATcg                                                                                  >  1:869693/1‑139 (MQ=255)                                                cTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATcgc                                                                                 >  2:358248/1‑139 (MQ=255)                                                 tGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATcgcg                                                                                <  1:7045/139‑1 (MQ=255)                                                  gAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg                                                                               >  1:170182/1‑139 (MQ=255)                                                   aGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCggg                                                                              <  2:869693/139‑1 (MQ=255)                                                        gggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCAc                                                                                                           <  1:1438441/105‑1 (MQ=255)                                                        gggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCAc                                                                                                           >  2:1438441/1‑105 (MQ=255)                                                            agaaTAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGt                                                                     <  2:170182/139‑1 (MQ=255)                                                             gaaTAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTc                                                                    <  2:293426/139‑1 (MQ=255)                                                               aTAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTg                                                                  <  1:1540301/139‑1 (MQ=255)                                                                  gTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGACGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCtt                                                               <  1:358248/139‑1 (MQ=255)                                                                    ggCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtct                                                             >  1:1309267/1‑139 (MQ=255)                                                                         aGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCAt                                                        <  2:1399041/139‑1 (MQ=255)                                                                           aTTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCt                                                                                                      <  2:1308452/91‑1 (MQ=255)                                                                           aTTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCt                                                                                                      >  1:1308452/1‑91 (MQ=255)                                                                           aTTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtct                                                             >  1:1241392/1‑132 (MQ=255)                                                                           aTTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtct                                                             <  2:1241392/132‑1 (MQ=255)                                                                           aTTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCt                                                      >  1:972821/1‑139 (MQ=255)                                                                             tGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCt                                                      <  1:776712/137‑1 (MQ=255)                                                                             tGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCt                                                      >  2:776712/1‑137 (MQ=255)                                                                               tAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATg                                                  >  2:835860/1‑139 (MQ=255)                                                                                aGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGt                                                 <  1:743135/139‑1 (MQ=255)                                                                                  cGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                               <  2:1309267/139‑1 (MQ=255)                                                                                     aaTTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg                                                                               >  2:364189/1‑104 (MQ=255)                                                                                     aaTTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg                                                                               <  1:364189/104‑1 (MQ=255)                                                                                       ttCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACt                                          >  2:1149713/1‑139 (MQ=255)                                                                                         cTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGGGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTa                                        <  2:1016399/139‑1 (MQ=255)                                                                                          tGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGTGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTaa                                       <  2:972821/139‑1 (MQ=255)                                                                                          tGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTaa                                       <  1:778043/139‑1 (MQ=255)                                                                                            cAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAaca                                     >  1:1139250/1‑139 (MQ=255)                                                                                                 gtgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGc                                                                                             >  2:364659/1‑78 (MQ=255)                                                                                                 gtgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGc                                                                                             <  1:364659/78‑1 (MQ=255)                                                                                                  tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTca                               >  1:411331/1‑139 (MQ=255)                                                                                                    tACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTcaac                             <  2:1139250/139‑1 (MQ=255)                                                                                                       tCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc                                                                                                             <  2:770373/56‑1 (MQ=255)                                                                                                       tCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc                                                                                                             >  1:770373/1‑56 (MQ=255)                                                                                                         aCTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaatat                        <  2:1400201/139‑1 (MQ=255)                                                                                                         aCTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaat                          <  2:344280/137‑1 (MQ=255)                                                                                                         aCTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaat                          >  1:344280/1‑137 (MQ=255)                                                                                                          cTAGCTTAAAAAAACTCGGCGCATATCTTTAGTCCGGATGAAAAATcc                                                                                                                  <  1:947012/48‑1 (MQ=255)                                                                                                          cTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATcc                                                                                                                  >  2:947012/1‑48 (MQ=255)                                                                                                              cTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc                                                                                                             <  1:1116171/49‑1 (MQ=255)                                                                                                              cTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc                                                                                                             >  2:1116171/1‑49 (MQ=255)                                                                                                              cTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAAcaca                                   >  2:776753/1‑123 (MQ=255)                                                                                                              cTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAAcaca                                   <  1:776753/123‑1 (MQ=255)                                                                                                                        cTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCt                                                                   >  2:412637/1‑81 (MQ=255)                                                                                                                        cTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCt                                                                   <  1:412637/81‑1 (MQ=255)                                                                                                                        cTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTcgc         >  2:1014897/1‑139 (MQ=255)                                                                                                                               cATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACAt  <  1:1149713/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    ATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACAT  >  NC_000913/3478459‑3478723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑