Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,556,033 A→G Y273H (TAC→CAC)  rtcA ← RNA 3'‑terminal phosphate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,556,0330AG100.0% 149.6 / NA 43Y273H (TAC→CAC) rtcARNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/23);  total (20/23)

CTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACC  >  NC_000913/3555899‑3556153
                                                                                                                                      |                                                                                                                        
cTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGt                                                                                                                      >  1:912437/1‑139 (MQ=255)
       accGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACct                                                                                                               <  2:820409/139‑1 (MQ=255)
       accGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACct                                                                                                               <  2:408226/139‑1 (MQ=255)
       accGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACct                                                                                                               <  2:36667/139‑1 (MQ=255)
        ccGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACctc                                                                                                              >  1:368652/1‑139 (MQ=255)
         cgcgATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACctct                                                                                                             <  1:143674/139‑1 (MQ=255)
             atatTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCg                                                                                                                       <  2:515202/125‑1 (MQ=255)
             atatTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCg                                                                                                                       >  1:515202/1‑125 (MQ=255)
                 tGGTCAGCAG‑‑GGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACt                                                                                                   <  2:246780/139‑1 (MQ=255)
                   gTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACt                                                                                                   >  1:199716/1‑139 (MQ=255)
                       gcagATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCTCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTGCACCTCTTTCACCAACTGTGc                                                                                               <  1:616100/139‑1 (MQ=255)
                        cagATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGcc                                                                                              >  2:1457080/1‑139 (MQ=255)
                        cagATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCACACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGcc                                                                                              <  2:912437/139‑1 (MQ=255)
                             ggCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAac                                                                                         >  2:556047/1‑139 (MQ=255)
                                aTGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAaccac                                                                                      <  1:556047/139‑1 (MQ=255)
                                                        cccccGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCAcc                                                              <  2:368652/139‑1 (MQ=255)
                                                          cccGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACcga                                                            <  1:1457080/139‑1 (MQ=255)
                                                             gcgcCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACcgacga                                                         <  2:555929/139‑1 (MQ=255)
                                                             gcgcCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCa                                                                                           >  1:1318414/1‑104 (MQ=255)
                                                             gcgcCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCa                                                                                           <  2:1318414/104‑1 (MQ=255)
                                                                   gccAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcgcg                                                                       >  1:116871/1‑119 (MQ=255)
                                                                   gccAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcgcg                                                                       <  2:116871/119‑1 (MQ=255)
                                                                            aTCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCgg                                          <  2:199716/139‑1 (MQ=255)
                                                                                             gTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCAcc                                                                                                       <  1:1264393/61‑1 (MQ=255)
                                                                                             gTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCAcc                                                                                                       >  2:1264393/1‑61 (MQ=255)
                                                                                                    aGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCaa                  >  2:1057844/1‑139 (MQ=255)
                                                                                                        atTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCga              >  1:703140/1‑139 (MQ=255)
                                                                                                          tCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCtt                                                                                                            >  2:1118523/1‑43 (MQ=255)
                                                                                                          tCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCtt                                                                                                            <  1:1118523/43‑1 (MQ=255)
                                                                                                          tCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCgaga            <  1:1057844/139‑1 (MQ=255)
                                                                                                            ccccACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACg          >  1:254400/1‑139 (MQ=255)
                                                                                                               cACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTa       >  1:813242/1‑139 (MQ=255)
                                                                                                                aCCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTAt      >  1:1486480/1‑139 (MQ=255)
                                                                                                                 ccgccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACcga                                                            <  2:1518756/84‑1 (MQ=255)
                                                                                                                 ccgccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACcga                                                            >  1:1518756/1‑84 (MQ=255)
                                                                                                                 ccgccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAg                 <  2:1525631/127‑1 (MQ=255)
                                                                                                                 ccgccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAg                 >  1:1525631/1‑127 (MQ=255)
                                                                                                                 ccgccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTAtt     >  1:1401057/1‑139 (MQ=255)
                                                                                                                    ccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGtt                                             >  1:68228/1‑96 (MQ=255)
                                                                                                                    ccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGtt                                             <  2:68228/96‑1 (MQ=255)
                                                                                                                    ccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTAcc  <  2:254400/139‑1 (MQ=255)
                                                                                                                    ccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGCGCCGCAACCACCTCGGCACTGACGCGCTTTTCAcc                                                              <  2:478337/79‑1 (MQ=255)
                                                                                                                    ccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGCGCCGCAACCACCTCGGCACTGACGCGCTTTTCAcc                                                              >  1:478337/1‑79 (MQ=255)
                                                                                                                                      |                                                                                                                        
CTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACC  >  NC_000913/3555899‑3556153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑