Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 340,089 G→A intergenic (+346/‑76) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913340,0890GA100.0% 170.2 / NA 54intergenic (+346/‑76)yahG/yahIDUF1116 family protein/carbamate kinase‑like protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/29);  total (25/29)

GCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCA  >  NC_000913/339953‑340204
                                                                                                                                        |                                                                                                                   
gcgTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGc                                                                                                                   <  2:49282/139‑1 (MQ=255)
   tGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTg                                                                                                                <  2:742446/139‑1 (MQ=255)
                   ccTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATc                                                                                                >  2:76284/1‑139 (MQ=255)
                       cGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGc                                                                                            >  1:693307/1‑139 (MQ=255)
                             gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTg                                                                                      >  2:408558/1‑139 (MQ=255)
                                cgAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                   >  2:1334691/1‑139 (MQ=255)
                                 gagaATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGt                                                                                  <  1:109791/139‑1 (MQ=255)
                                 gagaATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGt                                                                                  >  2:1609150/1‑139 (MQ=255)
                                         gTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATa                                                                          <  2:1163226/139‑1 (MQ=255)
                                          tAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATaa                                                                         <  1:408558/139‑1 (MQ=255)
                                           aGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGt                                                                                  <  2:1529790/129‑1 (MQ=255)
                                           aGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGt                                                                                  >  1:1529790/1‑129 (MQ=255)
                                               cTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                    <  1:1334691/139‑1 (MQ=255)
                                               cTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                    <  2:1420951/139‑1 (MQ=255)
                                                  aTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                             >  1:210673/1‑111 (MQ=255)
                                                  aTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                             <  2:210673/111‑1 (MQ=255)
                                                  aTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCa                                                                 >  1:1095858/1‑139 (MQ=255)
                                                      gCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCct                                                             <  2:503605/139‑1 (MQ=255)
                                                            cgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAg                                                       >  2:46256/1‑139 (MQ=255)
                                                                tCAGGCATTTGTCACCCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACGCCATCACCCTCTGTAGGGGa                                                   <  2:1095858/139‑1 (MQ=255)
                                                                     cATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                              <  1:1609150/139‑1 (MQ=255)
                                                                        ttGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaacaa                                           >  1:1041761/1‑139 (MQ=255)
                                                                         tGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaacaac                                          >  2:13935/1‑139 (MQ=255)
                                                                           tCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAAcccc                                                                     >  2:535138/1‑110 (MQ=255)
                                                                           tCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAAcccc                                                                     <  1:535138/110‑1 (MQ=255)
                                                                              ccATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCGGACTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGaa                                     >  1:1089858/1‑139 (MQ=255)
                                                                                  tGCCGGATGTGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                             <  1:571413/79‑1 (MQ=255)
                                                                                  tGCCGGATGTGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                             >  2:571413/1‑79 (MQ=255)
                                                                                  tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                   <  2:1046309/89‑1 (MQ=255)
                                                                                  tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                   >  1:1046309/1‑89 (MQ=255)
                                                                                  tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCg                                                     >  1:812390/1‑119 (MQ=255)
                                                                                  tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCg                                                     <  2:812390/119‑1 (MQ=255)
                                                                                  tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAagag                                 >  2:885479/1‑139 (MQ=255)
                                                                                         tGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAcc                                                               <  2:241343/102‑1 (MQ=255)
                                                                                         tGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAcc                                                               >  1:241343/1‑102 (MQ=255)
                                                                                            ggcgTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCg                       <  1:76284/139‑1 (MQ=255)
                                                                                               gTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTg                    <  2:693307/139‑1 (MQ=255)
                                                                                                  aaCGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTtg                             >  1:1227670/1‑127 (MQ=255)
                                                                                                  aaCGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCa                 <  1:885479/139‑1 (MQ=255)
                                                                                                  aaCGCCTTATCCGGCCTACGAGTGGAGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTtg                             <  2:1227670/127‑1 (MQ=255)
                                                                                                            ccGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCaa       >  1:1216359/1‑139 (MQ=255)
                                                                                                               gCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAg                                                                                                           >  1:1085054/1‑36 (MQ=255)
                                                                                                               gCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAg                                                                                                           <  2:1085054/36‑1 (MQ=255)
                                                                                                               gCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                              <  2:1460809/97‑1 (MQ=255)
                                                                                                               gCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                              >  1:1460809/1‑97 (MQ=255)
                                                                                                                ccTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCg                                                     >  1:626769/1‑89 (MQ=255)
                                                                                                                ccTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCg                                                     <  2:626769/89‑1 (MQ=255)
                                                                                                                 cTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa  <  2:1041761/139‑1 (MQ=255)
                                                                                                                 cTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa  <  2:1514887/139‑1 (MQ=255)
                                                                                                                 cTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa  <  1:1194311/139‑1 (MQ=255)
                                                                                                                    cGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa  >  1:1386993/1‑136 (MQ=255)
                                                                                                                    cGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa  <  2:1386993/136‑1 (MQ=255)
                                                                                                                          gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAg                                                                                              >  1:1327814/1‑38 (MQ=14)
                                                                                                                          gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAg                                                                                              <  2:1327814/38‑1 (MQ=14)
                                                                                                                                        |                                                                                                                   
GCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCA  >  NC_000913/339953‑340204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: