Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,589,820 T→C S70G (AGC→GGC)  ugpE ← sn‑glycerol‑3‑phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,589,8200TC100.0% 270.1 / NA 76S70G (AGC→GGC) ugpEsn‑glycerol‑3‑phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/35);  total (41/35)

AGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAA  >  NC_000913/3589685‑3589955
                                                                                                                                       |                                                                                                                                       
agaagaagaGGTTACGTAGCGGAAAACGAAACCAGACAATGGTAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                      <  2:1212485/139‑1 (MQ=255)
agaagaagaGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                      <  2:939447/139‑1 (MQ=255)
          gTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTAcc                                                                                                                            >  2:692643/1‑139 (MQ=255)
            tACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTAcccc                                                                                                                          >  2:325593/1‑139 (MQ=255)
            tACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTAcccc                                                                                                                          >  1:710200/1‑139 (MQ=255)
            tACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTAcccc                                                                                                                          >  1:490102/1‑139 (MQ=255)
               gTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGtt                                                                                                                       >  2:636792/1‑139 (MQ=255)
                    ggAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCAccc                                                                                                                  >  1:1012222/1‑139 (MQ=255)
                             aaCCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGAtgttgt                                                                                                         >  1:1237165/1‑139 (MQ=255)
                             aaCCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGAtgttgt                                                                                                         >  2:75669/1‑139 (MQ=255)
                                  gACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATg                                                                                                    <  1:75669/139‑1 (MQ=255)
                                     aaTGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGttt                                                                                                 >  2:945363/1‑139 (MQ=255)
                                             aTGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCcagcag                                                                                         <  2:1237165/139‑1 (MQ=255)
                                               gCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGAt                                                                                       <  2:490102/139‑1 (MQ=255)
                                                   agagCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCg                                                                                                                                    <  2:1081082/90‑1 (MQ=255)
                                                   agagCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCg                                                                                                                                    >  1:1081082/1‑90 (MQ=255)
                                                   agagCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGttt                                                                                                 <  1:1474610/125‑1 (MQ=255)
                                                   agagCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGttt                                                                                                 >  2:1474610/1‑125 (MQ=255)
                                                   agagCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGAtgtgt                                                                                   >  2:617362/1‑139 (MQ=255)
                                                    gagCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGAtgtgtg                                                                                  <  2:1297591/139‑1 (MQ=255)
                                                       cATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTGTTCCAGCAGATGTGTGCCg                                                                               <  1:580873/139‑1 (MQ=255)
                                                         tCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCggg                                                                             >  2:1227755/1‑139 (MQ=255)
                                                          cGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGAtg                                                                                      >  1:419932/1‑129 (MQ=255)
                                                          cGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGAtg                                                                                      <  2:419932/129‑1 (MQ=255)
                                                          cGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCgggg                                                                            >  1:157732/1‑139 (MQ=255)
                                                                ggTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCg                                                                                                                         >  1:476051/1‑88 (MQ=255)
                                                                ggTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCg                                                                                                                         <  2:476051/88‑1 (MQ=255)
                                                                ggTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGc                                                                      >  1:668159/1‑139 (MQ=255)
                                                                ggTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGc                                                                      >  1:840020/1‑139 (MQ=255)
                                                                  tAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGt                                                                    >  2:944275/1‑139 (MQ=255)
                                                                     ttttGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCAt                                                                 <  2:1012222/139‑1 (MQ=255)
                                                                        tGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCgg                                                              <  1:636792/139‑1 (MQ=255)
                                                                          ccGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGAtgttgt                                                                                                         <  2:1195915/94‑1 (MQ=255)
                                                                          ccGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGAtgttgt                                                                                                         >  1:1195915/1‑94 (MQ=255)
                                                                          ccGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGgcg                                                            <  1:1227755/139‑1 (MQ=255)
                                                                          ccGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGgcg                                                            >  1:1318895/1‑139 (MQ=255)
                                                                            gAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGttt                                                                                                 <  1:800299/100‑1 (MQ=255)
                                                                            gAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGttt                                                                                                 >  2:800299/1‑100 (MQ=255)
                                                                               cGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                      >  1:1076270/1‑60 (MQ=255)
                                                                               cGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                      <  2:1076270/60‑1 (MQ=255)
                                                                               cGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCa                                                       <  1:325593/139‑1 (MQ=255)
                                                                                         aaCGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGtt                                                                                                                       <  1:982241/65‑1 (MQ=255)
                                                                                         aaCGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGACGCGCCATTCGTGCCTACCCCGtt                                                                                                                       >  2:982241/1‑65 (MQ=255)
                                                                                           cGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGt                                                                                                                        <  2:652846/62‑1 (MQ=255)
                                                                                           cGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGt                                                                                                                        >  1:652846/1‑62 (MQ=255)
                                                                                           cGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGgc                                                             >  1:483036/1‑121 (MQ=255)
                                                                                           cGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGgc                                                             <  2:483036/121‑1 (MQ=255)
                                                                                              cATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTa                                        >  1:805704/1‑139 (MQ=255)
                                                                                               aTCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTAcc                                                                                                                            <  2:80261/54‑1 (MQ=255)
                                                                                               aTCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTAcc                                                                                                                            >  1:80261/1‑54 (MQ=255)
                                                                                                     aaGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACgg                                                <  1:204032/124‑1 (MQ=255)
                                                                                                     aaGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACgg                                                >  2:204032/1‑124 (MQ=255)
                                                                                                                cAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACa                      >  1:948417/1‑139 (MQ=255)
                                                                                                                  aCATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACaaa                    >  2:394783/1‑139 (MQ=255)
                                                                                                                   cATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGt                                           <  1:1405166/115‑1 (MQ=255)
                                                                                                                   cATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGt                                           >  2:1405166/1‑115 (MQ=255)
                                                                                                                     tCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCa                                                                                                                     >  2:929795/1‑39 (MQ=255)
                                                                                                                     tCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCa                                                                                                                     <  1:929795/39‑1 (MQ=255)
                                                                                                                      ccGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGAAGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGcc                <  1:1511357/139‑1 (MQ=255)
                                                                                                                        gCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGgcgc                                                           <  2:198345/94‑1 (MQ=255)
                                                                                                                        gCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGgcgc                                                           >  1:198345/1‑94 (MQ=255)
                                                                                                                        gCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCAc              >  2:1166325/1‑139 (MQ=255)
                                                                                                                        gCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCAc              >  2:1270456/1‑139 (MQ=255)
                                                                                                                         ccAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCAt                                                                 <  2:771225/87‑1 (MQ=255)
                                                                                                                         ccAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCAt                                                                 >  1:771225/1‑87 (MQ=255)
                                                                                                                           aGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTa           <  2:157732/139‑1 (MQ=255)
                                                                                                                             aaCGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACa         >  2:1595736/1‑139 (MQ=255)
                                                                                                                              aCGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAg        <  1:617362/139‑1 (MQ=255)
                                                                                                                                ggCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCg      <  2:1318895/139‑1 (MQ=255)
                                                                                                                                 gcgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCgg     >  2:1418968/1‑139 (MQ=255)
                                                                                                                                  cgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCcag                                                                                            >  2:1403702/1‑51 (MQ=255)
                                                                                                                                  cgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCcag                                                                                            <  1:1403702/51‑1 (MQ=255)
                                                                                                                                  cgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACaa                     >  2:113095/1‑122 (MQ=255)
                                                                                                                                  cgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACaa                     <  1:113095/122‑1 (MQ=255)
                                                                                                                                    cgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCg      <  2:861140/135‑1 (MQ=255)
                                                                                                                                    cgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGaa  <  1:1270456/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
AGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAA  >  NC_000913/3589685‑3589955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: